Restriction map for
pF1KB8891
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaII AviII
AvrII BalI BamHI BanII BbrPI
BbvCI BciVI BfrI BglII BlnI
BmyI BpmI BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsgI BsiHKAI
BsmBI BsmFI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BsrI BssSI Bst1107I BstAPI
BstBI BstXI BstYI BstZ17I Bsu36I
Cfr13I ClaI CpoI DraII DraIII
DrdI DsaI Eam1105I Eco47III Eco81I
EcoNI EcoO109I EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MflI
MluI MluNI MroI MscI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 68 enzymes) #
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AcsI 303 GAATTC [GA]^AATT[CT]
AcyI 90 GGCGCC G[GA]^CG[CT]C
ApoI 303 GAATTC [GA]^AATT[CT]
Asp700I 602 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 85 CCCGAG C^[CT]CG[GA]G
BanI 90 GGCGCC G^G[CT][GA]CC
BbeI 90 GGCGCC GGCGC^C
BbsI 100 GAAGAC GAAGAC(2/6)
BcgI 536 GCAGACCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 174 TGATCA T^GATCA
BcnI 345 CCGGG CC^[GC]GG
BfaI 260 CTAG C^TAG
BglI 329 GCCAGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 138 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 138 GCTGAGC GC^T[ACGT]AGC
BpuAI 100 GAAGAC GAAGAC(2/6)
BsaHI 90 GGCGCC G[GA]^CG[CT]C
BsiEI 114 CGGCCG CG[GA][CT]^CG
BsiWI 464 CGTACG C^GTACG
BsmAI 457 GAGAC GTCTC(1/5)
BsmI 606 GCATTC GAATGC(1/-1)
BsoBI 85 CCCGAG C^[CT]CG[GA]G
BsrFI 70 GCCGGT [GA]^CCGG[CT]
BssHII 125 GCGCGC G^CGCGC
BstEII 230 GGTCACC G^GT[ACGT]ACC
BstPI 230 GGTCACC G^GT[ACGT]ACC
CelII 138 GCTGAGC GC^T[ACGT]AGC
Cfr10I 70 GCCGGT [GA]^CCGG[CT]
DpnII 175 GATC ^GATC
DraI 372 TTTAAA TTT^AAA
EaeI 114 CGGCCG [CT]^GGCC[GA]
EagI 114 CGGCCG C^GGCCG
EarI 263 GAAGAG CTCTTC(1/4)
EclXI 114 CGGCCG C^GGCCG
Eco52I 114 CGGCCG C^GGCCG
EcoO65I 230 GGTCACC G^GT[ACGT]ACC
EcoRI 303 GAATTC G^AATTC
EcoT14I 407 CCAAGG C^C[AT][AT]GG
FbaI 174 TGATCA T^GATCA
HaeII 90 GGCGCC [GA]GCGC^[CT]
HgaI 49 GCGTC GACGC(5/10)
Hin1I 90 GGCGCC G[GA]^CG[CT]C
KasI 90 GGCGCC G^GCGCC
Ksp632I 263 GAAGAG CTCTTC(1/4)
MaeI 260 CTAG C^TAG
MaeII 463 ACGT A^CGT
MboI 175 GATC ^GATC
MslI 370 CATTTAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 90 GGCGCC GG^CGCC
NciI 345 CCGGG CC^[GC]GG
NdeII 175 GATC ^GATC
NlaIV 90 GGCGCC GG[ACGT]^[ACGT]CC
NspI 529 GCATGT [GA]CATG^[CT]
PleI 148 GACTC GAGTC(4/5)
PshAI 385 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau3AI 175 GATC ^GATC
SexAI 581 ACCTGGT A^CC[AT]GGT
SfaNI 448 GATGC GCATC(5/9)
SfoI 90 GGCGCC GGC^GCC
SspI 474 AATATT AAT^ATT
StuI 79 AGGCCT AGG^CCT
StyI 407 CCAAGG C^C[AT][AT]GG
SwaI 371 ATTTAAAT ATTT^AAAT
TaqI 182 TCGA T^CGA
TfiI 273 GAATC G^A[AT]TC
Tsp509I 304 AATT ^AATT
TthHB8I 182 TCGA T^CGA
XmnI 602 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 429 GTAC GT^AC
465 GTAC
AluI 288 AGCT AG^CT
453 AGCT
BsiYI 71 CCGGTAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
611 CCGCTAAAAGG
BslI 71 CCGGTAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
611 CCGCTAAAAGG
BsrBI 105 CCGCTC CCGCTC(-3/-3)
268 GAGCGG
BstF5I 12 GGATG GGATG(2/0)
625 GGATG
BstNI 30 CCTGG CC^[AT]GG
582 CCTGG
EcoRII 30 CCTGG ^CC[AT]GG
582 CCTGG
FokI 12 GGATG GGATG(9/13)
625 GGATG
HapII 71 CCGG C^CGG
345 CCGG
HinfI 148 GACTC G^A[ACGT]TC
273 GAATC
HpaII 71 CCGG C^CGG
345 CCGG
HphI 507 GGTGA GGTGA(8/7)
232 TCACC
MboII 100 GAAGA GAAGA(8/7)
263 GAAGA
MspI 71 CCGG C^CGG
345 CCGG
MvaI 30 CCTGG CC^[AT]GG
582 CCTGG
NlaIII 192 CATG CATG^
530 CATG
PstI 284 CTGCAG CTGCA^G
432 CTGCAG
PvuII 287 CAGCTG CAG^CTG
452 CAGCTG
RsaI 429 GTAC GT^AC
465 GTAC
SfcI 284 CTGCAG C^T[GA][CT]AG
432 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AccII AciI BbvI BsaJI BssKI
BstUI Cac8I CfoI DdeI FauI
Fnu4HI HaeIII HhaI HinP1I ItaI
MaeIII MnlI MseI MspA1I MvnI
MwoI ScrFI Tru9I TseI Tsp45I
TspRI