Restriction map for
pF1KB8904
##################################
# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbsI BcgI BciVI
BclI BfrI BglI BglII BlnI
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI MamI MfeI
MluI MluNI MroI MscI MseI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccII 286 CGCG CG^CG
AfaI 111 GTAC GT^AC
AlwI 93 GATCC GGATC(4/5)
AlwNI 595 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 654 GGACC G^G[AT]CC
BbrPI 440 CACGTG CAC^GTG
BbvCI 508 GCTGAGG CCTCAGC(-5/-2)
BfaI 16 CTAG C^TAG
BlpI 153 GCTGAGC GC^T[ACGT]AGC
BpmI 451 CTGGAG CTGGAG(16/14)
Bpu1102I 153 GCTGAGC GC^T[ACGT]AGC
BsaAI 440 CACGTG [CT]AC^GT[GA]
BseRI 34 CTCCTC GAGGAG(10/8)
BsgI 673 CTGCAC GTGCAG(16/14)
BspMI 71 ACCTGC ACCTGC(4/8)
BstEII 471 GGTGACC G^GT[ACGT]ACC
BstPI 471 GGTGACC G^GT[ACGT]ACC
BstUI 286 CGCG CG^CG
BstXI 461 CCAAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 92 AGATCC [GA]^GATC[CT]
CelII 153 GCTGAGC GC^T[ACGT]AGC
DraII 653 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 171 GACCAACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 535 CTCTTC CTCTTC(1/4)
EcoNI 72 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 653 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 471 GGTGACC G^GT[ACGT]ACC
EcoT14I 77 CCAAGG C^C[AT][AT]GG
HaeII 612 GGCGCT [GA]GCGC^[CT]
Ksp632I 535 CTCTTC CTCTTC(1/4)
KspI 285 CCGCGG CCGC^GG
MaeI 16 CTAG C^TAG
MaeII 441 ACGT A^CGT
MflI 92 AGATCC [GA]^GATC[CT]
MslI 363 CACCAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 286 CGCG CG^CG
NspI 236 ACATGC [GA]CATG^[CT]
PflMI 594 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 440 CACGTG CAC^GTG
PmlI 440 CACGTG CAC^GTG
PpuMI 653 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 595 CAGCTG CAG^CTG
RsaI 111 GTAC GT^AC
SacII 285 CCGCGG CCGC^GG
SfcI 616 CTACAG C^T[GA][CT]AG
StyI 77 CCAAGG C^C[AT][AT]GG
Tsp45I 472 GTGAC ^GT[GC]AC
Van91I 594 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 92 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AspHI 370 GTGCTC G[AT]GC[AT]^C
387 GTGCTC
BcnI 99 CCGGG CC^[GC]GG
215 CCCGG
BmyI 370 GTGCTC G[AGT]GC[ACT]^C
387 GTGCTC
BsiHKAI 370 GTGCTC G[AT]GC[AT]^C
387 GTGCTC
BsmAI 456 GTCTC GTCTC(1/5)
694 GTCTC
BsmFI 101 GGGAC GGGAC(10/14)
352 GTCCC
Bsp1286I 370 GTGCTC G[AGT]GC[ACT]^C
387 GTGCTC
BsrI 104 ACTGG ACTGG(1/-1)
200 ACTGG
BstF5I 684 CATCC GGATG(2/0)
249 GGATG
CfoI 60 GCGC GCG^C
613 GCGC
Cfr13I 309 GGCCC G^G[ACGT]CC
654 GGACC
DpnII 13 GATC ^GATC
93 GATC
DsaI 285 CCGCGG C^C[GA][CT]GG
318 CCGTGG
FokI 684 CATCC GGATG(9/13)
249 GGATG
HapII 99 CCGG C^CGG
216 CCGG
HhaI 60 GCGC GCG^C
613 GCGC
HinP1I 60 GCGC G^CGC
613 GCGC
HpaII 99 CCGG C^CGG
216 CCGG
MaeIII 197 GTAAC ^GT[ACGT]AC
472 GTGAC
MboI 13 GATC ^GATC
93 GATC
MboII 90 GAAGA GAAGA(8/7)
536 TCTTC
MspA1I 285 CCGCGG C[AC]G^C[GT]G
595 CAGCTG
MspI 99 CCGG C^CGG
216 CCGG
NciI 99 CCGGG CC^[GC]GG
215 CCCGG
NdeII 13 GATC ^GATC
93 GATC
NlaIII 237 CATG CATG^
393 CATG
NlaIV 68 GGAACC GG[ACGT]^[ACGT]CC
654 GGACCC
Sau3AI 13 GATC ^GATC
93 GATC
Sau96I 309 GGCCC G^G[ACGT]CC
654 GGACC
StuI 30 AGGCCT AGG^CCT
296 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BssKI BstNI Cac8I DdeI
EcoRII FauI Fnu4HI HaeIII HinfI
HphI ItaI MnlI MvaI MwoI
PleI ScrFI TseI TspRI