Restriction map for
pF1KB8908
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# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaII AviII
AvrII BalI BamHI BbrPI BbsI
BbvCI BciVI BclI BfrI BglI
BlnI BpmI BpuAI BsaAI BsaBI
BsaWI BseAI BsgI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HindIII HpaI HphI KpnI KspI
MaeIII MamI MfeI MluI MluNI
MroI MscI MslI MspA1I MunI
MvnI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tsp45I Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcsI 259 AAATTC [GA]^AATT[CT]
AfaI 117 GTAC GT^AC
ApaI 121 GGGCCC GGGCC^C
ApoI 259 AAATTC [GA]^AATT[CT]
AvaI 647 CCCGAG C^[CT]CG[GA]G
BbeI 135 GGCGCC GGCGC^C
BbvI 239 GCTGC GCAGC(8/12)
BcgI 317 CGAGCGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 490 CTAG C^TAG
BglII 581 AGATCT A^GATCT
BsaI 426 GAGACC GGTCTC(1/5)
BsoBI 647 CCCGAG C^[CT]CG[GA]G
BstYI 581 AGATCT [GA]^GATC[CT]
DpnII 582 GATC ^GATC
HgaI 250 GACGC GACGC(5/10)
HincII 16 GTCAAC GT[CT]^[GA]AC
HindII 16 GTCAAC GT[CT]^[GA]AC
HinfI 49 GAGTC G^A[ACGT]TC
KasI 135 GGCGCC G^GCGCC
MaeI 490 CTAG C^TAG
MboI 582 GATC ^GATC
MflI 581 AGATCT [GA]^GATC[CT]
NaeI 43 GCCGGC GCC^GGC
NarI 135 GGCGCC GG^CGCC
NcoI 418 CCATGG C^CATGG
NdeII 582 GATC ^GATC
NgoMIV 43 GCCGGC G^CCGGC
PflMI 105 CCATGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 49 GAGTC GAGTC(4/5)
RsaI 117 GTAC GT^AC
SacI 3 GAGCTC GAGCT^C
Sau3AI 582 GATC ^GATC
SfaNI 197 GCATC GCATC(5/9)
SfoI 135 GGCGCC GGC^GCC
TseI 239 GCTGC G^C[AT]GC
Tsp509I 260 AATT ^AATT
TspRI 450 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 105 CCATGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 581 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 350 CCGC CCGC(-3/-1)
128 GCGG
AcyI 135 GGCGCC G[GA]^CG[CT]C
250 GACGCC
AluI 4 AGCT AG^CT
238 AGCT
AspHI 3 GAGCTC G[AT]GC[AT]^C
228 GTGCTC
BanI 135 GGCGCC G^G[CT][GA]CC
187 GGCACC
BlpI 234 GCTTAGC GC^T[ACGT]AGC
304 GCTCAGC
Bpu1102I 234 GCTTAGC GC^T[ACGT]AGC
304 GCTCAGC
BsaHI 135 GGCGCC G[GA]^CG[CT]C
250 GACGCC
BsiHKAI 3 GAGCTC G[AT]GC[AT]^C
228 GTGCTC
BsmBI 398 CGTCTC CGTCTC(1/5)
248 GAGACG
BstF5I 422 GGATG GGATG(2/0)
602 GGATG
BstNI 54 CCTGG CC^[AT]GG
555 CCTGG
CelII 234 GCTTAGC GC^T[ACGT]AGC
304 GCTCAGC
Cfr13I 121 GGGCC G^G[ACGT]CC
122 GGCCC
EarI 685 GAAGAG CTCTTC(1/4)
697 GAAGAG
EcoRII 54 CCTGG ^CC[AT]GG
555 CCTGG
EcoT14I 418 CCATGG C^C[AT][AT]GG
482 CCTTGG
Fnu4HI 128 GCGGC GC^[ACGT]GC
239 GCTGC
FokI 422 GGATG GGATG(9/13)
602 GGATG
HaeII 135 GGCGCC [GA]GCGC^[CT]
276 GGCGCT
Hin1I 135 GGCGCC G[GA]^CG[CT]C
250 GACGCC
ItaI 128 GCGGC GC^[ACGT]GC
239 GCTGC
Ksp632I 685 GAAGAG CTCTTC(1/4)
697 GAAGAG
MaeII 164 ACGT A^CGT
531 ACGT
MseI 385 TTAA T^TAA
509 TTAA
MvaI 54 CCTGG CC^[AT]GG
555 CCTGG
Sau96I 121 GGGCC G^G[ACGT]CC
122 GGCCC
StyI 418 CCATGG C^C[AT][AT]GG
482 CCTTGG
Tru9I 385 TTAA T^TAA
509 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
BanII BcnI BmyI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrFI BssKI Cac8I CfoI Cfr10I
DdeI DsaI HaeIII HapII HhaI
HinP1I HpaII MboII MnlI MspI
MwoI NciI NlaIII NlaIV ScrFI