Restriction map for
pF1KB8909
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BanII BbeI BbsI
BbvCI BcgI BciVI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsiWI BsmBI
BsmI Bsp1407I BspDI BspLU11I BspMI
BsrDI BsrFI BsrGI BssSI Bst1107I
BstAPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI Eam1105I EarI Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KasI KpnI Ksp632I MaeI
MamI MboII MfeI MluI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NruI NsiI NspI PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuII
RsrII SacI SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp509I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccIII 90 TCCGGA T^CCGGA
AcyI 94 GACGCC G[GA]^CG[CT]C
AfaI 216 GTAC GT^AC
Aor51HI 629 AGCGCT AGC^GCT
Asp700I 627 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 136 GAGCAC G[AT]GC[AT]^C
AvaI 147 CCCGGG C^[CT]CG[GA]G
BanI 69 GGTGCC G^G[CT][GA]CC
BbrPI 525 CACGTG CAC^GTG
BclI 695 TGATCA T^GATCA
BpmI 386 CTGGAG CTGGAG(16/14)
BsaAI 525 CACGTG [CT]AC^GT[GA]
BsaHI 94 GACGCC G[GA]^CG[CT]C
BseAI 90 TCCGGA T^CCGGA
BsiHKAI 136 GAGCAC G[AT]GC[AT]^C
BsmAI 339 GAGAC GTCTC(1/5)
BsmFI 152 GGGAC GGGAC(10/14)
BsoBI 147 CCCGGG C^[CT]CG[GA]G
BspEI 90 TCCGGA T^CCGGA
BspHI 635 TCATGA T^CATGA
BssHII 356 GCGCGC G^CGCGC
BstBI 54 TTCGAA TT^CGAA
DsaI 5 CCGCGG C^C[GA][CT]GG
EagI 8 CGGCCG C^GGCCG
EclXI 8 CGGCCG C^GGCCG
Eco47III 629 AGCGCT AGC^GCT
Eco52I 8 CGGCCG C^GGCCG
EcoT14I 131 CCAAGG C^C[AT][AT]GG
FbaI 695 TGATCA T^GATCA
HgaI 94 GACGC GACGC(5/10)
Hin1I 94 GACGCC G[GA]^CG[CT]C
KspI 5 CCGCGG CCGC^GG
MroI 90 TCCGGA T^CCGGA
MseI 542 TTAA T^TAA
MslI 499 CATACAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NotI 7 GCGGCCGC GC^GGCCGC
NspV 54 TTCGAA TT^CGAA
PflMI 36 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 315 GAGTC GAGTC(4/5)
PmaCI 525 CACGTG CAC^GTG
PmlI 525 CACGTG CAC^GTG
PvuI 703 CGATCG CGAT^CG
RcaI 635 TCATGA T^CATGA
RsaI 216 GTAC GT^AC
SacII 5 CCGCGG CCGC^GG
SfcI 438 CTACAG C^T[GA][CT]AG
SfuI 54 TTCGAA TT^CGAA
SmaI 147 CCCGGG CCC^GGG
StyI 131 CCAAGG C^C[AT][AT]GG
TfiI 304 GAATC G^A[AT]TC
Tru9I 542 TTAA T^TAA
Van91I 36 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 191 CCAAGAGCTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 147 CCCGGG C^CCGGG
XmaI 147 CCCGGG C^CCGGG
XmnI 627 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AluI 196 AGCT AG^CT
566 AGCT
AlwNI 37 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
441 CAGTGGCTG
AvaII 204 GGACC G^G[AT]CC
584 GGACC
BalI 529 TGGCCA TGG^CCA
719 TGGCCA
BamHI 280 GGATCC G^GATCC
345 GGATCC
BmyI 136 GAGCAC G[AGT]GC[ACT]^C
432 GTGCCC
BsaWI 90 TCCGGA [AT]^CCGG[AT]
581 ACCGGA
BsiEI 8 CGGCCG CG[GA][CT]^CG
703 CGATCG
Bsp1286I 136 GAGCAC G[AGT]GC[ACT]^C
432 GTGCCC
BstF5I 225 CATCC GGATG(2/0)
382 CATCC
BstYI 280 GGATCC [GA]^GATC[CT]
345 GGATCC
DdeI 116 CTGAG C^T[ACGT]AG
558 CTCAG
FokI 225 CATCC GGATG(9/13)
382 CATCC
HaeII 629 AGCGCT [GA]GCGC^[CT]
714 GGCGCT
HinfI 304 GAATC G^A[ACGT]TC
315 GAGTC
MaeII 293 ACGT A^CGT
526 ACGT
MflI 280 GGATCC [GA]^GATC[CT]
345 GGATCC
MluNI 529 TGGCCA TGG^CCA
719 TGGCCA
MscI 529 TGGCCA TGG^CCA
719 TGGCCA
XhoII 280 GGATCC [GA]^GATC[CT]
345 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AlwI BbvI BcnI
BsaJI BseRI BsgI BsiYI BslI
BsrBI BsrI BssKI BstEII BstNI
BstPI BstUI Cac8I CfoI Cfr13I
DpnII EaeI EcoO65I EcoRII FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeIII MboI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI TaqI TseI
Tsp45I TspRI TthHB8I