Restriction map for
pF1KB8922
##################################
# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BbrPI BbsI BciVI
BclI BfrI BlnI BpuAI BsaAI
BsaI BsaWI BseAI BsiEI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BsrI BssSI BstAPI BstBI
BstEII BstPI BstXI ClaI DraI
DraIII EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HpaI
KpnI Ksp632I KspI MaeII MaeIII
MfeI MluI MroI MseI MslI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp45I Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccI 600 GTATAC GT^[AC][GT]AC
AcyI 325 GGCGCC G[GA]^CG[CT]C
AfaI 9 GTAC GT^AC
AlwNI 613 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 424 CCTGAGG CC^T[ACGT]AGG
ApaI 587 GGGCCC GGGCC^C
BalI 485 TGGCCA TGG^CCA
BamHI 105 GGATCC G^GATCC
BanI 325 GGCGCC G^G[CT][GA]CC
BbeI 325 GGCGCC GGCGC^C
BcgI 671 GCATCGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 460 CTAG C^TAG
BglI 504 GCCCGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 716 AGATCT A^GATCT
BlpI 444 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 444 GCTAAGC GC^T[ACGT]AGC
BsaBI 711 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 325 GGCGCC G[GA]^CG[CT]C
BsgI 303 CTGCAC GTGCAG(16/14)
BssHII 219 GCGCGC G^CGCGC
Bst1107I 600 GTATAC GTA^TAC
BstZ17I 600 GTATAC GTA^TAC
Bsu36I 424 CCTGAGG CC^T[ACGT]AGG
CelII 444 GCTAAGC GC^T[ACGT]AGC
CpoI 592 CGGACCG CG^G[AT]CCG
DraII 586 AGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 157 GACCTCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 485 TGGCCA [CT]^GGCC[GA]
Eco81I 424 CCTGAGG CC^T[ACGT]AGG
EcoNI 533 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 586 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 482 CCATGG C^C[AT][AT]GG
Hin1I 325 GGCGCC G[GA]^CG[CT]C
HincII 166 GTCAAC GT[CT]^[GA]AC
HindII 166 GTCAAC GT[CT]^[GA]AC
HindIII 544 AAGCTT A^AGCTT
HphI 389 TCACC GGTGA(8/7)
KasI 325 GGCGCC G^GCGCC
MaeI 460 CTAG C^TAG
MamI 711 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 747 GAAGA GAAGA(8/7)
MluNI 485 TGGCCA TGG^CCA
MscI 485 TGGCCA TGG^CCA
NarI 325 GGCGCC GG^CGCC
NcoI 482 CCATGG C^CATGG
PflMI 612 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 785 CAGCTG CAG^CTG
RsaI 9 GTAC GT^AC
RsrII 592 CGGACCG CG^G[AT]CCG
SfaNI 671 GCATC GCATC(5/9)
SfcI 782 CTACAG C^T[GA][CT]AG
SfoI 325 GGCGCC GGC^GCC
StyI 482 CCATGG C^C[AT][AT]GG
TspRI 75 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 612 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 106 GATCC GGATC(4/5)
105 GGATC
AspHI 3 GAGCTC G[AT]GC[AT]^C
213 GAGCTC
AvaII 156 GGACC G^G[AT]CC
593 GGACC
BbvCI 159 CCTCAGC CCTCAGC(-5/-2)
365 CCTCAGC
BseRI 174 CTCCTC GAGGAG(10/8)
183 CTCCTC
BsiHKAI 3 GAGCTC G[AT]GC[AT]^C
213 GAGCTC
BsmFI 294 GTCCC GGGAC(10/14)
345 GTCCC
BsrBI 171 CCGCTC CCGCTC(-3/-3)
90 GAGCGG
BsrFI 124 ACCGGC [GA]^CCGG[CT]
731 ACCGGC
BstF5I 152 GGATG GGATG(2/0)
536 GGATG
BstYI 105 GGATCC [GA]^GATC[CT]
716 AGATCT
Cfr10I 124 ACCGGC [GA]^CCGG[CT]
731 ACCGGC
DsaI 321 CCACGG C^C[GA][CT]GG
482 CCATGG
FokI 152 GGATG GGATG(9/13)
536 GGATG
HgaI 164 GCGTC GACGC(5/10)
412 GCGTC
HinfI 292 GAGTC G^A[ACGT]TC
516 GACTC
MflI 105 GGATCC [GA]^GATC[CT]
716 AGATCT
MspA1I 110 CCGCTG C[AC]G^C[GT]G
785 CAGCTG
PleI 516 GACTC GAGTC(4/5)
292 GAGTC
SacI 3 GAGCTC GAGCT^C
213 GAGCTC
TaqI 268 TCGA T^CGA
674 TCGA
TthHB8I 268 TCGA T^CGA
674 TCGA
XhoII 105 GGATCC [GA]^GATC[CT]
716 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccII AciI AluI AvaI BanII
BbvI BcnI BmyI BpmI BsaJI
BsiYI BslI BsoBI Bsp1286I BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HpaII ItaI MboI MnlI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
TseI Tsp509I