################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BlnI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiHKAI BsmBI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HinfI HpaI KpnI Ksp632I MaeII MamI MboII MfeI MluNI MroI MscI MseI MslI MunI NaeI NdeI NgoMIV NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SapI SbfI SexAI SfaNI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 369 GTCTAC GT^[AC][GT]AC AflIII 383 ACGCGT A^C[GA][CT]GT ApaI 292 GGGCCC GGGCC^C Asp700I 486 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 767 CCCGAG C^[CT]CG[GA]G AvaII 45 GGACC G^G[AT]CC BfaI 475 CTAG C^TAG BglII 572 AGATCT A^GATCT BlpI 262 GCTCAGC GC^T[ACGT]AGC Bpu1102I 262 GCTCAGC GC^T[ACGT]AGC BsiWI 200 CGTACG C^GTACG BsmAI 561 GAGAC GTCTC(1/5) BsmFI 634 GGGAC GGGAC(10/14) BsoBI 767 CCCGAG C^[CT]CG[GA]G Bsp1407I 107 TGTACA T^GTACA BsrGI 107 TGTACA T^GTACA BsrI 396 CCAGT ACTGG(1/-1) BstF5I 362 GGATG GGATG(2/0) BstYI 572 AGATCT [GA]^GATC[CT] CelII 262 GCTCAGC GC^T[ACGT]AGC DpnII 573 GATC ^GATC EcoT14I 358 CCATGG C^C[AT][AT]GG FokI 362 GGATG GGATG(9/13) HphI 246 TCACC GGTGA(8/7) KspI 230 CCGCGG CCGC^GG MaeI 475 CTAG C^TAG MaeIII 685 GTGAC ^GT[ACGT]AC MboI 573 GATC ^GATC MflI 572 AGATCT [GA]^GATC[CT] MluI 383 ACGCGT A^CGCGT NcoI 358 CCATGG C^CATGG NdeII 573 GATC ^GATC NotI 831 GCGGCCGC GC^GGCCGC PpuMI 44 AGGACCC [GA]G^G[AT]CC[CT] PshAI 636 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 230 CCGCGG CCGC^GG Sau3AI 573 GATC ^GATC ScaI 17 AGTACT AGT^ACT SspBI 107 TGTACA T^GTACA StyI 358 CCATGG C^C[AT][AT]GG Tsp45I 685 GTGAC ^GT[GC]AC TspRI 397 CAGTG CA[GC]TG[ACGT][ACGT]^ XhoII 572 AGATCT [GA]^GATC[CT] XmnI 486 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### BanII 292 GGGCCC G[GA]GC[CT]^C 331 GAGCCC BglI 285 GCCCGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 825 GCCTCGGCGGC BsiEI 517 CGGCCG CG[GA][CT]^CG 832 CGGCCG BssHII 164 GCGCGC G^CGCGC 462 GCGCGC DraII 44 AGGACCC [GA]G^G[ACGT]CC[CT] 292 GGGCCCT DsaI 230 CCGCGG C^C[GA][CT]GG 358 CCATGG EagI 517 CGGCCG C^GGCCG 832 CGGCCG EclXI 517 CGGCCG C^GGCCG 832 CGGCCG Eco52I 517 CGGCCG C^GGCCG 832 CGGCCG EcoO109I 44 AGGACCC [GA]G^G[ACGT]CC[CT] 292 GGGCCCT ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AccII AciI AcyI AfaI AluI BanI BbeI BbvI BcnI BmyI BpmI BsaHI BsaJI BseRI BsiYI BslI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI EaeI EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI KasI MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIII NlaIV PvuII RsaI Sau96I ScrFI SfoI TseI