##################################
# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BlnI BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiHKAI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI Eam1105I EarI Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HinfI HpaI KpnI
Ksp632I MaeII MamI MboII MfeI
MluNI MroI MscI MseI MslI
MunI NaeI NdeI NgoMIV NheI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SalI SapI SbfI SexAI SfaNI
SfcI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 369 GTCTAC GT^[AC][GT]AC
AflIII 383 ACGCGT A^C[GA][CT]GT
ApaI 292 GGGCCC GGGCC^C
Asp700I 486 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 767 CCCGAG C^[CT]CG[GA]G
AvaII 45 GGACC G^G[AT]CC
BfaI 475 CTAG C^TAG
BglII 572 AGATCT A^GATCT
BlpI 262 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 262 GCTCAGC GC^T[ACGT]AGC
BsiWI 200 CGTACG C^GTACG
BsmAI 561 GAGAC GTCTC(1/5)
BsmFI 634 GGGAC GGGAC(10/14)
BsoBI 767 CCCGAG C^[CT]CG[GA]G
Bsp1407I 107 TGTACA T^GTACA
BsrGI 107 TGTACA T^GTACA
BsrI 396 CCAGT ACTGG(1/-1)
BstF5I 362 GGATG GGATG(2/0)
BstYI 572 AGATCT [GA]^GATC[CT]
CelII 262 GCTCAGC GC^T[ACGT]AGC
DpnII 573 GATC ^GATC
EcoT14I 358 CCATGG C^C[AT][AT]GG
FokI 362 GGATG GGATG(9/13)
HphI 246 TCACC GGTGA(8/7)
KspI 230 CCGCGG CCGC^GG
MaeI 475 CTAG C^TAG
MaeIII 685 GTGAC ^GT[ACGT]AC
MboI 573 GATC ^GATC
MflI 572 AGATCT [GA]^GATC[CT]
MluI 383 ACGCGT A^CGCGT
NcoI 358 CCATGG C^CATGG
NdeII 573 GATC ^GATC
NotI 831 GCGGCCGC GC^GGCCGC
PpuMI 44 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 636 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 230 CCGCGG CCGC^GG
Sau3AI 573 GATC ^GATC
ScaI 17 AGTACT AGT^ACT
SspBI 107 TGTACA T^GTACA
StyI 358 CCATGG C^C[AT][AT]GG
Tsp45I 685 GTGAC ^GT[GC]AC
TspRI 397 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 572 AGATCT [GA]^GATC[CT]
XmnI 486 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
BanII 292 GGGCCC G[GA]GC[CT]^C
331 GAGCCC
BglI 285 GCCCGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
825 GCCTCGGCGGC
BsiEI 517 CGGCCG CG[GA][CT]^CG
832 CGGCCG
BssHII 164 GCGCGC G^CGCGC
462 GCGCGC
DraII 44 AGGACCC [GA]G^G[ACGT]CC[CT]
292 GGGCCCT
DsaI 230 CCGCGG C^C[GA][CT]GG
358 CCATGG
EagI 517 CGGCCG C^GGCCG
832 CGGCCG
EclXI 517 CGGCCG C^GGCCG
832 CGGCCG
Eco52I 517 CGGCCG C^GGCCG
832 CGGCCG
EcoO109I 44 AGGACCC [GA]G^G[ACGT]CC[CT]
292 GGGCCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
BanI BbeI BbvI BcnI BmyI
BpmI BsaHI BsaJI BseRI BsiYI
BslI Bsp1286I BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI EaeI
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII ItaI KasI MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NlaIII NlaIV PvuII RsaI
Sau96I ScrFI SfoI TseI