Restriction map for
pF1KB8952
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstXI CelII ClaI
CpoI DraIII DrdI Eam1105I Eco47III
EcoNI EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI MaeII
MamI MfeI MluI MroI MunI
NarI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 605 GTATAC GT^[AC][GT]AC
AocI 528 CCTCAGG CC^T[ACGT]AGG
AvrII 180 CCTAGG C^CTAGG
BalI 588 TGGCCA TGG^CCA
BamHI 131 GGATCC G^GATCC
BglI 433 GCCGCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 180 CCTAGG C^CTAGG
BsmAI 10 GTCTC GTCTC(1/5)
BsmBI 9 CGTCTC CGTCTC(1/5)
Bst1107I 605 GTATAC GTA^TAC
BstEII 102 GGTGACC G^GT[ACGT]ACC
BstF5I 899 GGATG GGATG(2/0)
BstNI 39 CCTGG CC^[AT]GG
BstPI 102 GGTGACC G^GT[ACGT]ACC
BstYI 131 GGATCC [GA]^GATC[CT]
BstZ17I 605 GTATAC GTA^TAC
Bsu36I 528 CCTCAGG CC^T[ACGT]AGG
DdeI 529 CTCAG C^T[ACGT]AG
EarI 163 CTCTTC CTCTTC(1/4)
Eco81I 528 CCTCAGG CC^T[ACGT]AGG
EcoO65I 102 GGTGACC G^GT[ACGT]ACC
EcoRI 731 GAATTC G^AATTC
EcoRII 39 CCTGG ^CC[AT]GG
FokI 899 GGATG GGATG(9/13)
HaeII 276 AGCGCC [GA]GCGC^[CT]
Ksp632I 163 CTCTTC CTCTTC(1/4)
KspI 506 CCGCGG CCGC^GG
MflI 131 GGATCC [GA]^GATC[CT]
MluNI 588 TGGCCA TGG^CCA
MscI 588 TGGCCA TGG^CCA
MslI 610 CACTTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 39 CCTGG CC^[AT]GG
NaeI 463 GCCGGC GCC^GGC
NcoI 585 CCATGG C^CATGG
NgoMIV 463 GCCGGC G^CCGGC
PpuMI 416 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 3 GACTGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 452 CTGCAG CTGCA^G
PvuII 325 CAGCTG CAG^CTG
SacII 506 CCGCGG CCGC^GG
SfcI 452 CTGCAG C^T[GA][CT]AG
TfiI 712 GATTC G^A[AT]TC
XcmI 591 CCAGCTTGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 131 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 348 GTAC GT^AC
949 GTAC
ApaI 89 GGGCCC GGGCC^C
423 GGGCCC
AvaII 138 GGACC G^G[AT]CC
417 GGTCC
BpmI 221 CTCCAG CTGGAG(16/14)
370 CTGGAG
BseRI 266 CTCCTC GAGGAG(10/8)
964 CTCCTC
BsmFI 16 GTCCC GGGAC(10/14)
418 GTCCC
BsrBI 31 CCGCTC CCGCTC(-3/-3)
402 GAGCGG
BsrI 896 ACTGG ACTGG(1/-1)
345 CCAGT
DraI 543 TTTAAA TTT^AAA
757 TTTAAA
HphI 102 GGTGA GGTGA(8/7)
674 TCACC
NlaIII 586 CATG CATG^
601 CATG
PleI 366 GAGTC GAGTC(4/5)
447 GAGTC
RsaI 348 GTAC GT^AC
949 GTAC
SmaI 143 CCCGGG CCC^GGG
420 CCCGGG
Tsp45I 103 GTGAC ^GT[GC]AC
893 GTCAC
XmaCI 143 CCCGGG C^CCGGG
420 CCCGGG
XmaI 143 CCCGGG C^CCGGG
420 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI AvaI BanII BbvI BcnI
BfaI BmyI BsaJI BsiEI BsiYI
BslI BsoBI Bsp1286I BsrFI BssKI
BstUI Cac8I CfoI Cfr10I Cfr13I
DpnII DraII DsaI EaeI EagI
EclXI Eco52I EcoO109I EcoT14I FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeI MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvnI MwoI NciI NdeII
NlaIV Sau3AI Sau96I ScrFI StyI
Tru9I TseI Tsp509I TspRI