Restriction map for
pF1KB8955
##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BbvCI BciVI BclI BcnI BfaI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaBI BsaI BseAI BseRI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HindIII KasI KpnI MaeI MamI
MfeI MluI MroI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII ScaI
SexAI SfoI SfuI SgrAI SmaI
SnaBI SpeI SspBI SspI StuI
StyI SwaI TfiI Tth111I Van91I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 301 GTCGAC GT^[AC][GT]AC
AcyI 117 GACGCC G[GA]^CG[CT]C
AlwI 687 GATCC GGATC(4/5)
BcgI 104 CGAACACTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 154 GCCGACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 611 AGATCT A^GATCT
BsaAI 28 TACGTG [CT]AC^GT[GA]
BsaHI 117 GACGCC G[GA]^CG[CT]C
BsaWI 875 ACCGGA [AT]^CCGG[AT]
BsiEI 152 CGGCCG CG[GA][CT]^CG
BsrBI 328 CCGCTC CCGCTC(-3/-3)
BsrI 705 ACTGG ACTGG(1/-1)
BstEII 740 GGTTACC G^GT[ACGT]ACC
BstPI 740 GGTTACC G^GT[ACGT]ACC
EagI 152 CGGCCG C^GGCCG
EclXI 152 CGGCCG C^GGCCG
Eco52I 152 CGGCCG C^GGCCG
EcoO65I 740 GGTTACC G^GT[ACGT]ACC
HaeII 33 GGCGCT [GA]GCGC^[CT]
HapII 876 CCGG C^CGG
Hin1I 117 GACGCC G[GA]^CG[CT]C
HinfI 414 GACTC G^A[ACGT]TC
HpaI 757 GTTAAC GTT^AAC
HpaII 876 CCGG C^CGG
KspI 89 CCGCGG CCGC^GG
MslI 711 CATGATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 876 CCGG C^CGG
PaeR7I 663 CTCGAG C^TCGAG
PleI 414 GACTC GAGTC(4/5)
SacI 270 GAGCTC GAGCT^C
SacII 89 CCGCGG CCGC^GG
SalI 301 GTCGAC G^TCGAC
SapI 171 GCTCTTC GCTCTTC(1/4)
SbfI 626 CCTGCAGG CCTGCA^GG
SfaNI 404 GCATC GCATC(5/9)
SfiI 153 GGCCGACGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 663 CTCGAG C^T[CT][GA]AG
SphI 750 GCATGC GCATG^C
Sse8387I 626 CCTGCAGG CCTGCA^GG
XhoI 663 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 27 GTAC GT^AC
970 GTAC
AspHI 259 GTGCTC G[AT]GC[AT]^C
270 GAGCTC
AvaI 359 CCCGAG C^[CT]CG[GA]G
663 CTCGAG
BanII 169 GGGCTC G[GA]GC[CT]^C
270 GAGCTC
BsgI 213 CTGCAC GTGCAG(16/14)
459 CTGCAC
BsiHKAI 259 GTGCTC G[AT]GC[AT]^C
270 GAGCTC
BsoBI 359 CCCGAG C^[CT]CG[GA]G
663 CTCGAG
BsrDI 375 CATTGC GCAATG(2/0)
811 GCAATG
BstF5I 180 CATCC GGATG(2/0)
474 GGATG
BstYI 611 AGATCT [GA]^GATC[CT]
686 AGATCC
DraII 355 GGGACCC [GA]G^G[ACGT]CC[CT]
389 GGGTCCT
EarI 172 CTCTTC CTCTTC(1/4)
235 CTCTTC
EcoO109I 355 GGGACCC [GA]G^G[ACGT]CC[CT]
389 GGGTCCT
FokI 180 CATCC GGATG(9/13)
474 GGATG
HgaI 19 GACGC GACGC(5/10)
117 GACGC
HincII 301 GTCGAC GT[CT]^[GA]AC
757 GTTAAC
HindII 301 GTCGAC GT[CT]^[GA]AC
757 GTTAAC
HphI 597 GGTGA GGTGA(8/7)
194 TCACC
Ksp632I 172 CTCTTC CTCTTC(1/4)
235 CTCTTC
MflI 611 AGATCT [GA]^GATC[CT]
686 AGATCC
MseI 608 TTAA T^TAA
758 TTAA
MspA1I 89 CCGCGG C[AC]G^C[GT]G
679 CAGCGG
NspI 750 GCATGC [GA]CATG^[CT]
837 ACATGC
PpuMI 355 GGGACCC [GA]G^G[AT]CC[CT]
389 GGGTCCT
PstI 112 CTGCAG CTGCA^G
627 CTGCAG
RsaI 27 GTAC GT^AC
970 GTAC
SfcI 112 CTGCAG C^T[GA][CT]AG
627 CTGCAG
TaqI 302 TCGA T^CGA
664 TCGA
Tru9I 608 TTAA T^TAA
758 TTAA
TthHB8I 302 TCGA T^CGA
664 TCGA
XhoII 611 AGATCT [GA]^GATC[CT]
686 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AluI AvaII BalI
BbvI BmyI BpmI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EcoRII FauI Fnu4HI HaeIII HhaI
HinP1I ItaI MaeII MaeIII MboI
MboII MluNI MnlI MscI MvaI
MvnI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI TseI Tsp45I
Tsp509I TspRI XcmI