Restriction map for
pF1KB8984
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI ApoI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BpmI BsaBI BsaI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrGI BssSI Bst1107I BstBI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII Eam1105I Eco81I EcoRI EcoRV
EcoT22I FbaI FspI HincII HindII
HindIII HpaI KpnI KspI MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MseI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfcI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TaqI Tru9I Tsp509I Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AlwNI 624 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 347 AGCGCT AGC^GCT
AscI 129 GGCGCGCC GG^CGCGCC
AspHI 750 GTGCTC G[AT]GC[AT]^C
BbeI 541 GGCGCC GGCGC^C
BbvCI 621 CCTCAGC CCTCAGC(-5/-2)
BlpI 360 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 360 GCTGAGC GC^T[ACGT]AGC
BsaAI 28 TACGTG [CT]AC^GT[GA]
BseRI 987 GAGGAG GAGGAG(10/8)
BsgI 12 GTGCAG GTGCAG(16/14)
BsiHKAI 750 GTGCTC G[AT]GC[AT]^C
BsmAI 950 GAGAC GTCTC(1/5)
BspHI 728 TCATGA T^CATGA
BsrI 165 ACTGG ACTGG(1/-1)
BstAPI 500 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 335 CCATGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 360 GCTGAGC GC^T[ACGT]AGC
DrdI 862 GACCCCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 445 CTCTTC CTCTTC(1/4)
Eco47III 347 AGCGCT AGC^GCT
EcoNI 980 CCTCCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 47 GGCCGGCC GGCCGG^CC
KasI 541 GGCGCC G^GCGCC
Ksp632I 445 CTCTTC CTCTTC(1/4)
NarI 541 GGCGCC GG^CGCC
NcoI 335 CCATGG C^CATGG
PshAI 732 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 728 TCATGA T^CATGA
ScaI 488 AGTACT AGT^ACT
SfoI 541 GGCGCC GGC^GCC
SmlI 907 CTCAAG C^T[CT][GA]AG
SphI 518 GCATGC GCATG^C
TfiI 7 GATTC G^A[AT]TC
XcmI 173 CCACCGACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AcyI 312 GGCGTC G[GA]^CG[CT]C
541 GGCGCC
AfaI 489 GTAC GT^AC
639 GTAC
AlwI 714 GATCC GGATC(4/5)
768 GATCC
AvaI 254 CCCGAG C^[CT]CG[GA]G
934 CTCGGG
BanI 187 GGCACC G^G[CT][GA]CC
541 GGCGCC
BbsI 871 GTCTTC GAAGAC(2/6)
895 GTCTTC
BglI 406 GCCCGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
656 GCCACCAGGGC
BpuAI 871 GTCTTC GAAGAC(2/6)
895 GTCTTC
BsaHI 312 GGCGTC G[GA]^CG[CT]C
541 GGCGCC
BsaWI 160 ACCGGA [AT]^CCGG[AT]
688 ACCGGA
BsmFI 818 GGGAC GGGAC(10/14)
992 GGGAC
BsoBI 254 CCCGAG C^[CT]CG[GA]G
934 CTCGGG
BspMI 179 ACCTGC ACCTGC(4/8)
1065 GCAGGT
BstEII 156 GGTCACC G^GT[ACGT]ACC
615 GGTCACC
BstPI 156 GGTCACC G^GT[ACGT]ACC
615 GGTCACC
DpnII 714 GATC ^GATC
768 GATC
EagI 206 CGGCCG C^GGCCG
679 CGGCCG
EclXI 206 CGGCCG C^GGCCG
679 CGGCCG
Eco52I 206 CGGCCG C^GGCCG
679 CGGCCG
EcoO65I 156 GGTCACC G^GT[ACGT]ACC
615 GGTCACC
EcoT14I 335 CCATGG C^C[AT][AT]GG
997 CCAAGG
Hin1I 312 GGCGTC G[GA]^CG[CT]C
541 GGCGCC
MaeII 29 ACGT A^CGT
1117 ACGT
MaeIII 157 GTCAC ^GT[ACGT]AC
616 GTCAC
MboI 714 GATC ^GATC
768 GATC
NaeI 48 GCCGGC GCC^GGC
565 GCCGGC
NdeII 714 GATC ^GATC
768 GATC
NgoMIV 48 GCCGGC G^CCGGC
565 GCCGGC
NspI 518 GCATGC [GA]CATG^[CT]
635 GCATGT
PleI 905 GACTC GAGTC(4/5)
952 GACTC
PpuMI 818 GGGACCT [GA]G^G[AT]CC[CT]
992 GGGACCC
PvuII 385 CAGCTG CAG^CTG
501 CAGCTG
RsaI 489 GTAC GT^AC
639 GTAC
Sau3AI 714 GATC ^GATC
768 GATC
SfaNI 277 GATGC GCATC(5/9)
107 GCATC
StyI 335 CCATGG C^C[AT][AT]GG
997 CCAAGG
Tsp45I 157 GTCAC ^GT[GC]AC
616 GTCAC
TspRI 734 CAGTG CA[GC]TG[ACGT][ACGT]^
807 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AluI ApaI AvaII
BanII BbvI BcnI BmyI BsaJI
BsiEI BsiYI BslI Bsp1286I BsrFI
BssHII BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV Sau96I
ScrFI TseI