################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BcgI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaHI BsaI BsiWI BsoBI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MfeI MluI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tsp509I Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccIII 584 TCCGGA T^CCGGA Aor51HI 1010 AGCGCT AGC^GCT ApaI 103 GGGCCC GGGCC^C Asp700I 1075 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 564 GAGCTC G[AT]GC[AT]^C BalI 209 TGGCCA TGG^CCA BbsI 241 GTCTTC GAAGAC(2/6) BbvCI 234 CCTCAGC CCTCAGC(-5/-2) BciVI 876 GGATAC GTATCC(6/5) BglII 278 AGATCT A^GATCT BpmI 903 CTCCAG CTGGAG(16/14) BpuAI 241 GTCTTC GAAGAC(2/6) BsaAI 994 TACGTG [CT]AC^GT[GA] BsaBI 732 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 584 TCCGGA [AT]^CCGG[AT] BseAI 584 TCCGGA T^CCGGA BseRI 795 GAGGAG GAGGAG(10/8) BsiEI 459 CGACCG CG[GA][CT]^CG BsiHKAI 564 GAGCTC G[AT]GC[AT]^C BsmAI 379 GAGAC GTCTC(1/5) BsmBI 379 GAGACG CGTCTC(1/5) BsmFI 118 GGGAC GGGAC(10/14) BsmI 399 GAATGC GAATGC(1/-1) Bsp1407I 422 TGTACA T^GTACA BspDI 928 ATCGAT AT^CGAT BspEI 584 TCCGGA T^CCGGA BsrGI 422 TGTACA T^GTACA ClaI 928 ATCGAT AT^CGAT Eco47III 1010 AGCGCT AGC^GCT EcoRV 18 GATATC GAT^ATC FauI 323 GCGGG CCCGC(4/6) MamI 732 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 209 TGGCCA TGG^CCA MroI 584 TCCGGA T^CCGGA MscI 209 TGGCCA TGG^CCA MseI 113 TTAA T^TAA PleI 46 GACTC GAGTC(4/5) PpuMI 118 GGGACCT [GA]G^G[AT]CC[CT] SacI 564 GAGCTC GAGCT^C SspBI 422 TGTACA T^GTACA TfiI 1137 GATTC G^A[AT]TC Tru9I 113 TTAA T^TAA XcmI 646 CCATCCCTCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1075 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccII 391 CGCG CG^CG 505 CGCG AlwI 312 GATCC GGATC(4/5) 783 GATCC AlwNI 429 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG 745 CAGGTGCTG BanII 103 GGGCCC G[GA]GC[CT]^C 564 GAGCTC BfaI 217 CTAG C^TAG 811 CTAG BspMI 744 GCAGGT ACCTGC(4/8) 1067 GCAGGT BstUI 391 CGCG CG^CG 505 CGCG BstYI 278 AGATCT [GA]^GATC[CT] 782 AGATCC DraII 102 AGGGCCC [GA]G^G[ACGT]CC[CT] 118 GGGACCT EaeI 209 TGGCCA [CT]^GGCC[GA] 296 TGGCCG EcoO109I 102 AGGGCCC [GA]G^G[ACGT]CC[CT] 118 GGGACCT EcoT14I 491 CCATGG C^C[AT][AT]GG 1097 CCATGG HaeII 73 AGCGCC [GA]GCGC^[CT] 1010 AGCGCT HapII 498 CCGG C^CGG 585 CCGG HgaI 381 GACGC GACGC(5/10) 239 GCGTC HinfI 46 GACTC G^A[ACGT]TC 1137 GATTC HpaII 498 CCGG C^CGG 585 CCGG MaeI 217 CTAG C^TAG 811 CTAG MaeII 620 ACGT A^CGT 995 ACGT MaeIII 91 GTCAC ^GT[ACGT]AC 622 GTCAC MboII 15 GAAGA GAAGA(8/7) 242 TCTTC MflI 278 AGATCT [GA]^GATC[CT] 782 AGATCC MspI 498 CCGG C^CGG 585 CCGG MvnI 391 CGCG CG^CG 505 CGCG NcoI 491 CCATGG C^CATGG 1097 CCATGG NspI 80 ACATGC [GA]CATG^[CT] 674 ACATGC PstI 1065 CTGCAG CTGCA^G 1147 CTGCAG StyI 491 CCATGG C^C[AT][AT]GG 1097 CCATGG Tsp45I 91 GTCAC ^GT[GC]AC 622 GTCAC TspRI 986 CACTG CA[GC]TG[ACGT][ACGT]^ 1106 CACTG XhoII 278 AGATCT [GA]^GATC[CT] 782 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AfaI AluI AvaII BbvI BmyI BsaJI BsgI BsiYI BslI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DsaI EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HphI ItaI MboI MnlI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI TthHB8I