Restriction map for
pF1KB8992
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BglI
BglII BlnI BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII Bst1107I BstBI BstF5I
BstUI BstXI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DrdI Eam1105I
EarI Eco47III Eco81I EcoRI EcoRV
EcoT22I FauI FbaI FokI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MamI MfeI MflI
MluI MluNI MroI MscI MunI
MvnI NarI NcoI NdeI NheI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tsp509I TspRI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AflII 585 CTTAAG C^TTAAG
AlwI 534 GATCC GGATC(4/5)
AspHI 85 GTGCTC G[AT]GC[AT]^C
BanI 123 GGTGCC G^G[CT][GA]CC
BcnI 450 CCCGG CC^[GC]GG
BfrI 585 CTTAAG C^TTAAG
BlpI 159 GCTGAGC GC^T[ACGT]AGC
BpmI 301 CTGGAG CTGGAG(16/14)
Bpu1102I 159 GCTGAGC GC^T[ACGT]AGC
BsaAI 271 TACGTG [CT]AC^GT[GA]
BsiHKAI 85 GTGCTC G[AT]GC[AT]^C
BspMI 389 ACCTGC ACCTGC(4/8)
BssSI 439 CACGAG CACGAG(-5/-1)
BstEII 330 GGTGACC G^GT[ACGT]ACC
BstPI 330 GGTGACC G^GT[ACGT]ACC
CelII 159 GCTGAGC GC^T[ACGT]AGC
DdeI 160 CTGAG C^T[ACGT]AG
DraIII 325 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 80 CCACGG C^C[GA][CT]GG
EcoNI 501 CCTCACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 330 GGTGACC G^GT[ACGT]ACC
EcoT14I 176 CCAAGG C^C[AT][AT]GG
HaeII 432 GGCGCT [GA]GCGC^[CT]
MseI 586 TTAA T^TAA
MslI 267 CATCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 450 CCCGG CC^[GC]GG
NlaIV 123 GGTGCC GG[ACGT]^[ACGT]CC
NotI 225 GCGGCCGC GC^GGCCGC
PpuMI 386 AGGACCT [GA]G^G[AT]CC[CT]
SfcI 243 CTACAG C^T[GA][CT]AG
StyI 176 CCAAGG C^C[AT][AT]GG
Tru9I 586 TTAA T^TAA
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AvaII 294 GGACC G^G[AT]CC
387 GGACC
BseRI 12 CTCCTC GAGGAG(10/8)
304 GAGGAG
BsiEI 68 CGGCCG CG[GA][CT]^CG
226 CGGCCG
BsrI 577 ACTGG ACTGG(1/-1)
33 CCAGT
BstAPI 182 GCATCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
384 GCAGGACCTGC
BstNI 213 CCAGG CC^[AT]GG
351 CCAGG
CfoI 433 GCGC GCG^C
482 GCGC
DpnII 153 GATC ^GATC
534 GATC
DraII 386 AGGACCT [GA]G^G[ACGT]CC[CT]
497 AGGGCCT
EagI 68 CGGCCG C^GGCCG
226 CGGCCG
EclXI 68 CGGCCG C^GGCCG
226 CGGCCG
Eco52I 68 CGGCCG C^GGCCG
226 CGGCCG
EcoO109I 386 AGGACCT [GA]G^G[ACGT]CC[CT]
497 AGGGCCT
EcoRII 213 CCAGG ^CC[AT]GG
351 CCAGG
HhaI 433 GCGC GCG^C
482 GCGC
HinP1I 433 GCGC G^CGC
482 GCGC
HinfI 64 GACTC G^A[ACGT]TC
280 GACTC
MaeIII 331 GTGAC ^GT[ACGT]AC
581 GTGAC
MboI 153 GATC ^GATC
534 GATC
MvaI 213 CCAGG CC^[AT]GG
351 CCAGG
NaeI 70 GCCGGC GCC^GGC
474 GCCGGC
NdeII 153 GATC ^GATC
534 GATC
NgoMIV 70 GCCGGC G^CCGGC
474 GCCGGC
NlaIII 51 CATG CATG^
513 CATG
PleI 64 GACTC GAGTC(4/5)
280 GACTC
PvuII 186 CAGCTG CAG^CTG
427 CAGCTG
Sau3AI 153 GATC ^GATC
534 GATC
SfaNI 182 GCATC GCATC(5/9)
263 GCATC
Tsp45I 331 GTGAC ^GT[GC]AC
581 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AluI AlwNI BbvI BmyI
BsaJI BsgI BsiYI BslI BsmFI
Bsp1286I BsrFI BssKI Cac8I Cfr10I
Cfr13I EaeI Fnu4HI HaeIII HapII
HpaII HphI ItaI MaeII MboII
MnlI MspA1I MspI MwoI Sau96I
ScrFI SmlI TseI