################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeII MamI MfeI MluI MunI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccIII 237 TCCGGA T^CCGGA AlwNI 1072 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 154 GAGCTC G[AT]GC[AT]^C AvaI 674 CCCGGG C^[CT]CG[GA]G BalI 825 TGGCCA TGG^CCA BbeI 926 GGCGCC GGCGC^C BbvCI 429 CCTCAGC CCTCAGC(-5/-2) BcgI 176 CGACACTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpmI 376 CTGGAG CTGGAG(16/14) BsaWI 237 TCCGGA [AT]^CCGG[AT] BseAI 237 TCCGGA T^CCGGA BsgI 1069 GTGCAG GTGCAG(16/14) BsiEI 766 CGATCG CG[GA][CT]^CG BsiHKAI 154 GAGCTC G[AT]GC[AT]^C BsmBI 85 GAGACG CGTCTC(1/5) BsmI 17 GAATGC GAATGC(1/-1) BsoBI 674 CCCGGG C^[CT]CG[GA]G BspEI 237 TCCGGA T^CCGGA BspMI 489 GCAGGT ACCTGC(4/8) BsrBI 135 CCGCTC CCGCTC(-3/-3) BssSI 603 CACGAG CACGAG(-5/-1) BstXI 297 CCACAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 619 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1011 CCATGG C^C[GA][CT]GG EcoT14I 1011 CCATGG C^C[AT][AT]GG KasI 926 GGCGCC G^GCGCC MluNI 825 TGGCCA TGG^CCA MroI 237 TCCGGA T^CCGGA MscI 825 TGGCCA TGG^CCA MseI 425 TTAA T^TAA MspA1I 895 CAGCGG C[AC]G^C[GT]G NaeI 923 GCCGGC GCC^GGC NarI 926 GGCGCC GG^CGCC NcoI 1011 CCATGG C^CATGG NgoMIV 923 GCCGGC G^CCGGC NheI 699 GCTAGC G^CTAGC PflMI 688 CCAAACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1074 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 766 CGATCG CGAT^CG SacI 154 GAGCTC GAGCT^C SfoI 926 GGCGCC GGC^GCC SmaI 674 CCCGGG CCC^GGG StuI 998 AGGCCT AGG^CCT StyI 1011 CCATGG C^C[AT][AT]GG TaqI 170 TCGA T^CGA Tru9I 425 TTAA T^TAA TthHB8I 170 TCGA T^CGA Van91I 688 CCAAACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1145 CCATGCTGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 674 CCCGGG C^CCGGG XmaI 674 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccII 60 CGCG CG^CG 123 CGCG AfaI 1152 GTAC GT^AC 1177 GTAC AlwI 294 GATCC GGATC(4/5) 252 GGATC ApaI 501 GGGCCC GGGCC^C 794 GGGCCC BciVI 315 GTATCC GTATCC(6/5) 1100 GTATCC BglI 331 GCCAGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 827 GCCATGATGGC BsmAI 85 GAGAC GTCTC(1/5) 606 GAGAC BsrFI 260 ACCGGC [GA]^CCGG[CT] 923 GCCGGC BstEII 10 GGTAACC G^GT[ACGT]ACC 816 GGTCACC BstPI 10 GGTAACC G^GT[ACGT]ACC 816 GGTCACC BstUI 60 CGCG CG^CG 123 CGCG BstYI 252 GGATCT [GA]^GATC[CT] 293 AGATCC Cfr10I 260 ACCGGC [GA]^CCGG[CT] 923 GCCGGC DraII 500 AGGGCCC [GA]G^G[ACGT]CC[CT] 501 GGGCCCT EarI 478 CTCTTC CTCTTC(1/4) 286 GAAGAG EcoO109I 500 AGGGCCC [GA]G^G[ACGT]CC[CT] 501 GGGCCCT EcoO65I 10 GGTAACC G^GT[ACGT]ACC 816 GGTCACC HaeII 323 AGCGCC [GA]GCGC^[CT] 926 GGCGCC HgaI 131 GACGC GACGC(5/10) 857 GACGC Ksp632I 478 CTCTTC CTCTTC(1/4) 286 GAAGAG MflI 252 GGATCT [GA]^GATC[CT] 293 AGATCC MvnI 60 CGCG CG^CG 123 CGCG NspI 245 ACATGC [GA]CATG^[CT] 750 GCATGT PleI 714 GACTC GAGTC(4/5) 729 GACTC PstI 184 CTGCAG CTGCA^G 464 CTGCAG RsaI 1152 GTAC GT^AC 1177 GTAC SapI 286 GAAGAGC GCTCTTC(1/4) 477 GCTCTTC SfaNI 437 GCATC GCATC(5/9) 885 GCATC SfcI 184 CTGCAG C^T[GA][CT]AG 464 CTGCAG SfiI 330 GGCCAGCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 826 GGCCATGATGGCC SmlI 310 CTTGAG C^T[CT][GA]AG 776 CTCAAG TfiI 3 GATTC G^A[AT]TC 111 GATTC XhoII 252 GGATCT [GA]^GATC[CT] 293 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AcyI AluI AvaII BanI BanII BbvI BcnI BfaI BmyI BsaHI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI Tsp45I Tsp509I TspRI