################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BbvCI BciVI BclI BfrI BglI BlnI BsaBI BsaWI BseAI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tsp509I TspRI Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AflIII 818 ACACGT A^C[GA][CT]GT Alw44I 295 GTGCAC G^TGCAC AlwNI 381 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 295 GTGCAC G^TGCAC BamHI 893 GGATCC G^GATCC BbrPI 819 CACGTG CAC^GTG BcgI 321 GCAGCTAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 308 CCGGG CC^[GC]GG BglII 665 AGATCT A^GATCT BlpI 285 GCTGAGC GC^T[ACGT]AGC BpmI 696 CTCCAG CTGGAG(16/14) Bpu1102I 285 GCTGAGC GC^T[ACGT]AGC BsaAI 819 CACGTG [CT]AC^GT[GA] BsaI 55 GAGACC GGTCTC(1/5) BseRI 568 GAGGAG GAGGAG(10/8) BsgI 372 CTGCAC GTGCAG(16/14) BspHI 479 TCATGA T^CATGA BsrBI 634 CCGCTC CCGCTC(-3/-3) BssSI 298 CACGAG CACGAG(-5/-1) BstXI 401 CCATAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 285 GCTGAGC GC^T[ACGT]AGC CpoI 779 CGGTCCG CG^G[AT]CCG DraIII 214 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 242 CCATGG C^C[GA][CT]GG EarI 649 CTCTTC CTCTTC(1/4) EcoT14I 242 CCATGG C^C[AT][AT]GG HapII 308 CCGG C^CGG HinfI 748 GACTC G^A[ACGT]TC HpaII 308 CCGG C^CGG Ksp632I 649 CTCTTC CTCTTC(1/4) MseI 998 TTAA T^TAA MslI 870 CATTGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 308 CCGG C^CGG NciI 308 CCGGG CC^[GC]GG NcoI 242 CCATGG C^CATGG PleI 748 GACTC GAGTC(4/5) PmaCI 819 CACGTG CAC^GTG PmlI 819 CACGTG CAC^GTG RcaI 479 TCATGA T^CATGA RsrII 779 CGGTCCG CG^G[AT]CCG SacI 913 GAGCTC GAGCT^C SapI 648 GCTCTTC GCTCTTC(1/4) SexAI 230 ACCTGGT A^CC[AT]GGT StyI 242 CCATGG C^C[AT][AT]GG Tru9I 998 TTAA T^TAA XcmI 680 CCAAGTTCCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcyI 311 GGCGCC G[GA]^CG[CT]C 619 GGCGCC BanI 311 GGCGCC G^G[CT][GA]CC 619 GGCGCC BanII 693 GGGCTC G[GA]GC[CT]^C 913 GAGCTC BbeI 311 GGCGCC GGCGC^C 619 GGCGCC BbsI 702 GAAGAC GAAGAC(2/6) 112 GTCTTC BfaI 325 CTAG C^TAG 1061 CTAG BpuAI 702 GAAGAC GAAGAC(2/6) 112 GTCTTC BsaHI 311 GGCGCC G[GA]^CG[CT]C 619 GGCGCC BsmFI 815 GGGAC GGGAC(10/14) 923 GTCCC BsrI 419 ACTGG ACTGG(1/-1) 659 ACTGG BstYI 665 AGATCT [GA]^GATC[CT] 893 GGATCC DdeI 286 CTGAG C^T[ACGT]AG 557 CTGAG HgaI 467 GCGTC GACGC(5/10) 713 GCGTC Hin1I 311 GGCGCC G[GA]^CG[CT]C 619 GGCGCC KasI 311 GGCGCC G^GCGCC 619 GGCGCC MaeI 325 CTAG C^TAG 1061 CTAG MflI 665 AGATCT [GA]^GATC[CT] 893 GGATCC NarI 311 GGCGCC GG^CGCC 619 GGCGCC PflMI 225 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 380 CCAGCATCTGG SfoI 311 GGCGCC GGC^GCC 619 GGCGCC Van91I 225 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 380 CCAGCATCTGG XhoII 665 AGATCT [GA]^GATC[CT] 893 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI AvaII BbvI BmyI BsaJI BsiHKAI BsiYI BslI BsmAI BsmBI Bsp1286I BspMI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DpnII EaeI EcoRII FauI Fnu4HI FokI HaeII HaeIII HhaI HinP1I HphI ItaI MaeII MaeIII MboI MboII MnlI MspA1I MvaI MvnI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I