################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI Alw44I AlwI ApaI ApaLI AscI AseI AsnI Asp718I AspI AvaI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Cfr10I ClaI CpoI DpnII DraIII DrdI DsaI EagI EclXI Eco52I EcoNI EcoO65I EcoRV EcoT14I FbaI FseI FspI HpaI KpnI KspI MamI MboI MflI MluI MroI MslI NaeI NcoI NdeII NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccII 126 CGCG CG^CG AflIII 562 ACACGT A^C[GA][CT]GT AhdI 886 GACTTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 1213 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 580 CCTGAGG CC^T[ACGT]AGG Aor51HI 71 AGCGCT AGC^GCT Asp700I 649 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 886 GACTTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 853 GAGCAC G[AT]GC[AT]^C BanI 993 GGCGCC G^G[CT][GA]CC BbeI 993 GGCGCC GGCGC^C BbsI 271 GTCTTC GAAGAC(2/6) BcnI 213 CCGGG CC^[GC]GG BlpI 168 GCTCAGC GC^T[ACGT]AGC BpmI 292 CTGGAG CTGGAG(16/14) Bpu1102I 168 GCTCAGC GC^T[ACGT]AGC BpuAI 271 GTCTTC GAAGAC(2/6) BsgI 1139 GTGCAG GTGCAG(16/14) BsiHKAI 853 GAGCAC G[AT]GC[AT]^C BspMI 389 ACCTGC ACCTGC(4/8) BstUI 126 CGCG CG^CG BstXI 426 CCATTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 580 CCTGAGG CC^T[ACGT]AGG CelII 168 GCTCAGC GC^T[ACGT]AGC DraI 453 TTTAAA TTT^AAA DraII 1257 AGGACCC [GA]G^G[ACGT]CC[CT] Eam1105I 886 GACTTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 71 AGCGCT AGC^GCT Eco81I 580 CCTGAGG CC^T[ACGT]AGG EcoO109I 1257 AGGACCC [GA]G^G[ACGT]CC[CT] EcoT22I 636 ATGCAT ATGCA^T FauI 799 GCGGG CCCGC(4/6) HapII 213 CCGG C^CGG HgaI 940 GACGC GACGC(5/10) HincII 16 GTTGAC GT[CT]^[GA]AC HindII 16 GTTGAC GT[CT]^[GA]AC HindIII 534 AAGCTT A^AGCTT HpaII 213 CCGG C^CGG KasI 993 GGCGCC G^GCGCC MfeI 1043 CAATTG C^AATTG MspA1I 842 CAGCGG C[AC]G^C[GT]G MspI 213 CCGG C^CGG MunI 1043 CAATTG C^AATTG MvnI 126 CGCG CG^CG NarI 993 GGCGCC GG^CGCC NciI 213 CCGGG CC^[GC]GG NdeI 551 CATATG CA^TATG NsiI 636 ATGCAT ATGCA^T NspI 195 ACATGC [GA]CATG^[CT] PflMI 1212 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1257 AGGACCC [GA]G^G[AT]CC[CT] SfcI 30 CTACAG C^T[GA][CT]AG SfoI 993 GGCGCC GGC^GCC SmlI 67 CTCAAG C^T[CT][GA]AG Tsp45I 559 GTGAC ^GT[GC]AC Van91I 1212 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 399 CCAAATGTTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 649 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcsI 739 GAATTC [GA]^AATT[CT] 1083 GAATTC AcyI 940 GACGCC G[GA]^CG[CT]C 993 GGCGCC AfaI 210 GTAC GT^AC 396 GTAC ApoI 739 GAATTC [GA]^AATT[CT] 1083 GAATTC AvaII 222 GGTCC G^G[AT]CC 1258 GGACC BalI 155 TGGCCA TGG^CCA 515 TGGCCA BanII 104 GGGCTC G[GA]GC[CT]^C 575 GAGCCC BbvI 1141 GCAGC GCAGC(8/12) 1238 GCAGC BfaI 721 CTAG C^TAG 904 CTAG BsaHI 940 GACGCC G[GA]^CG[CT]C 993 GGCGCC BseRI 1194 CTCCTC GAGGAG(10/8) 295 GAGGAG BsmAI 47 GAGAC GTCTC(1/5) 1163 GTCTC Cac8I 526 GCAGGC GC[ACGT]^[ACGT]GC 871 GCCAGC CfoI 72 GCGC GCG^C 994 GCGC EaeI 155 TGGCCA [CT]^GGCC[GA] 515 TGGCCA EarI 820 CTCTTC CTCTTC(1/4) 835 CTCTTC EcoRI 739 GAATTC G^AATTC 1083 GAATTC HaeII 71 AGCGCT [GA]GCGC^[CT] 993 GGCGCC HhaI 72 GCGC GCG^C 994 GCGC Hin1I 940 GACGCC G[GA]^CG[CT]C 993 GGCGCC HinP1I 72 GCGC G^CGC 994 GCGC HphI 558 GGTGA GGTGA(8/7) 806 TCACC Ksp632I 820 CTCTTC CTCTTC(1/4) 835 CTCTTC MaeI 721 CTAG C^TAG 904 CTAG MaeIII 464 GTAAC ^GT[ACGT]AC 559 GTGAC MluNI 155 TGGCCA TGG^CCA 515 TGGCCA MscI 155 TGGCCA TGG^CCA 515 TGGCCA NlaIV 993 GGCGCC GG[ACGT]^[ACGT]CC 1258 GGACCC RsaI 210 GTAC GT^AC 396 GTAC TseI 1141 GCAGC G^C[AT]GC 1238 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AciI AluI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BspHI BsrI BssKI BstF5I BstNI Cfr13I DdeI EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeII MboII MnlI MseI MvaI MwoI NlaIII PleI RcaI Sau96I ScrFI SfaNI TfiI Tru9I Tsp509I TspRI