################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BamHI BanI BbeI BbrPI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BssSI BstBI BstXI CelII Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SphI Sse8387I SspI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccI 783 GTATAC GT^[AC][GT]AC AflIII 863 ACGTGT A^C[GA][CT]GT Alw44I 928 GTGCAC G^TGCAC AlwI 1063 GATCC GGATC(4/5) AlwNI 703 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 552 CCTGAGG CC^T[ACGT]AGG ApaLI 928 GTGCAC G^TGCAC AspHI 928 GTGCAC G[AT]GC[AT]^C BalI 1122 TGGCCA TGG^CCA BanII 823 GAGCCC G[GA]GC[CT]^C BbsI 742 GAAGAC GAAGAC(2/6) BfaI 1015 CTAG C^TAG BglI 438 GCCCGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 185 AGATCT A^GATCT BpuAI 742 GAAGAC GAAGAC(2/6) BsiHKAI 928 GTGCAC G[AT]GC[AT]^C Bsp1407I 899 TGTACA T^GTACA BspMI 908 ACCTGC ACCTGC(4/8) BsrDI 366 CATTGC GCAATG(2/0) BsrGI 899 TGTACA T^GTACA Bst1107I 783 GTATAC GTA^TAC BstAPI 3 GCAGGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 82 GGTTACC G^GT[ACGT]ACC BstPI 82 GGTTACC G^GT[ACGT]ACC BstZ17I 783 GTATAC GTA^TAC Bsu36I 552 CCTGAGG CC^T[ACGT]AGG DraI 508 TTTAAA TTT^AAA DraII 905 GGGACCT [GA]G^G[ACGT]CC[CT] DraIII 16 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 53 CCGTGG C^C[GA][CT]GG EarI 675 GAAGAG CTCTTC(1/4) Eco81I 552 CCTGAGG CC^T[ACGT]AGG EcoO109I 905 GGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 82 GGTTACC G^GT[ACGT]ACC EcoT14I 788 CCTTGG C^C[AT][AT]GG HaeII 49 AGCGCC [GA]GCGC^[CT] HgaI 851 GCGTC GACGC(5/10) Ksp632I 675 GAAGAG CTCTTC(1/4) MaeI 1015 CTAG C^TAG MluNI 1122 TGGCCA TGG^CCA MscI 1122 TGGCCA TGG^CCA MslI 654 CATGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspI 659 GCATGT [GA]CATG^[CT] PflMI 1002 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 905 GGGACCT [GA]G^G[AT]CC[CT] PstI 709 CTGCAG CTGCA^G PvuII 497 CAGCTG CAG^CTG SfcI 709 CTGCAG C^T[GA][CT]AG SnaBI 217 TACGTA TAC^GTA SpeI 1014 ACTAGT A^CTAGT SspBI 899 TGTACA T^GTACA StuI 5 AGGCCT AGG^CCT StyI 788 CCTTGG C^C[AT][AT]GG TaqI 717 TCGA T^CGA TfiI 995 GATTC G^A[AT]TC TthHB8I 717 TCGA T^CGA Van91I 1002 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccII 60 CGCG CG^CG 925 CGCG AcsI 316 AAATTT [GA]^AATT[CT] 681 GAATTT ApoI 316 AAATTT [GA]^AATT[CT] 681 GAATTT AspI 719 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 847 GACAGCGTC BbvCI 69 GCTGAGG CCTCAGC(-5/-2) 912 GCTGAGG BcnI 878 CCCGG CC^[GC]GG 966 CCGGG BmyI 823 GAGCCC G[AGT]GC[ACT]^C 928 GTGCAC BpmI 490 CTCCAG CTGGAG(16/14) 1094 CTCCAG BsaAI 217 TACGTA [CT]AC^GT[GA] 832 TACGTG Bsp1286I 823 GAGCCC G[AGT]GC[ACT]^C 928 GTGCAC BstUI 60 CGCG CG^CG 925 CGCG BstYI 185 AGATCT [GA]^GATC[CT] 1062 AGATCC DpnII 186 GATC ^GATC 1063 GATC EaeI 56 TGGCCG [CT]^GGCC[GA] 1122 TGGCCA FauI 952 CCCGC CCCGC(4/6) 1056 GCGGG HphI 90 GGTGA GGTGA(8/7) 859 GGTGA MboI 186 GATC ^GATC 1063 GATC MflI 185 AGATCT [GA]^GATC[CT] 1062 AGATCC MspA1I 497 CAGCTG C[AC]G^C[GT]G 1054 CAGCGG MvnI 60 CGCG CG^CG 925 CGCG NciI 878 CCCGG CC^[GC]GG 966 CCGGG NdeII 186 GATC ^GATC 1063 GATC PflFI 719 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 847 GACAGCGTC PleI 118 GACTC GAGTC(4/5) 279 GACTC Sau3AI 186 GATC ^GATC 1063 GATC SexAI 86 ACCAGGT A^CC[AT]GGT 720 ACCTGGT Tsp509I 317 AATT ^AATT 682 AATT Tth111I 719 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 847 GACAGCGTC XcmI 563 CCATCTTCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1105 CCAGCGCAGAGCTGG XhoII 185 AGATCT [GA]^GATC[CT] 1062 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AfaI AluI AvaII BbvI BsaJI BseRI BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeII MaeIII MboII MnlI MseI MspI MvaI MwoI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI Tru9I TseI Tsp45I TspRI