################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvrII BalI BamHI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FseI HgaI HincII HindII HindIII HinfI HpaI KpnI KspI MaeI MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NcoI NdeI NdeII NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcyI 141 GGCGCC G[GA]^CG[CT]C AfaI 402 GTAC GT^AC AviII 290 TGCGCA TGC^GCA BanI 141 GGCGCC G^G[CT][GA]CC BanII 327 GAGCCC G[GA]GC[CT]^C BbeI 141 GGCGCC GGCGC^C BbvCI 97 CCTCAGC CCTCAGC(-5/-2) BmyI 327 GAGCCC G[AGT]GC[ACT]^C BsaHI 141 GGCGCC G[GA]^CG[CT]C BseRI 95 CTCCTC GAGGAG(10/8) BsmFI 112 GTCCC GGGAC(10/14) Bsp1286I 327 GAGCCC G[AGT]GC[ACT]^C BspMI 159 ACCTGC ACCTGC(4/8) BstAPI 345 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 421 TTCGAA TT^CGAA DraII 254 AGGACCT [GA]G^G[ACGT]CC[CT] DsaI 145 CCACGG C^C[GA][CT]GG EarI 178 GAAGAG CTCTTC(1/4) EcoO109I 254 AGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 431 CCAAGG C^C[AT][AT]GG EcoT22I 222 ATGCAT ATGCA^T FauI 164 CCCGC CCCGC(4/6) FspI 290 TGCGCA TGC^GCA HaeII 141 GGCGCC [GA]GCGC^[CT] Hin1I 141 GGCGCC G[GA]^CG[CT]C HphI 157 TCACC GGTGA(8/7) KasI 141 GGCGCC G^GCGCC Ksp632I 178 GAAGAG CTCTTC(1/4) MaeII 55 ACGT A^CGT MaeIII 156 GTCAC ^GT[ACGT]AC MboII 178 GAAGA GAAGA(8/7) NaeI 138 GCCGGC GCC^GGC NarI 141 GGCGCC GG^CGCC NgoMIV 138 GCCGGC G^CCGGC NsiI 222 ATGCAT ATGCA^T NspI 416 GCATGT [GA]CATG^[CT] NspV 421 TTCGAA TT^CGAA PpuMI 254 AGGACCT [GA]G^G[AT]CC[CT] PstI 352 CTGCAG CTGCA^G PvuII 349 CAGCTG CAG^CTG RsaI 402 GTAC GT^AC SfcI 352 CTGCAG C^T[GA][CT]AG SfoI 141 GGCGCC GGC^GCC SfuI 421 TTCGAA TT^CGAA SgrAI 137 CGCCGGCG C[GA]^CCGG[CT]G StyI 431 CCAAGG C^C[AT][AT]GG TaqI 422 TCGA T^CGA Tsp45I 156 GTCAC ^GT[GC]AC TthHB8I 422 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AccII 166 CGCG CG^CG 168 CGCG BsaJI 145 CCACGG C^C[ACGT][ACGT]GG 431 CCAAGG BsrFI 108 GCCGGT [GA]^CCGG[CT] 138 GCCGGC BstF5I 318 CATCC GGATG(2/0) 39 GGATG BstNI 252 CCAGG CC^[AT]GG 282 CCAGG BstUI 166 CGCG CG^CG 168 CGCG Cfr10I 108 GCCGGT [GA]^CCGG[CT] 138 GCCGGC EcoRII 252 CCAGG ^CC[AT]GG 282 CCAGG FokI 318 CATCC GGATG(9/13) 39 GGATG HaeIII 3 GGCC GG^CC 149 GGCC MvaI 252 CCAGG CC^[AT]GG 282 CCAGG MvnI 166 CGCG CG^CG 168 CGCG NlaIV 111 GGTCCC GG[ACGT]^[ACGT]CC 141 GGCGCC SfaNI 288 GATGC GCATC(5/9) 317 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AciI AluI AlwNI AvaII BbvI BcnI BsiYI BslI BssKI Cac8I CfoI Cfr13I DdeI Fnu4HI HapII HhaI HinP1I HpaII ItaI MnlI MspA1I MspI MwoI NciI NlaIII Sau96I ScrFI TseI