################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV FauI FseI FspI HaeII HgaI Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MroI MslI MspA1I MunI MvnI NaeI NarI NgoMIV NotI NruI NspI NspV PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AciI 1527 GCGG CCGC(-3/-1) AseI 1110 ATTAAT AT^TAAT AsnI 1110 ATTAAT AT^TAAT BalI 78 TGGCCA TGG^CCA BanII 131 GGGCTC G[GA]GC[CT]^C BbvI 1403 GCAGC GCAGC(8/12) BciVI 1563 GTATCC GTATCC(6/5) BclI 1443 TGATCA T^GATCA BcnI 129 CCGGG CC^[GC]GG BglII 227 AGATCT A^GATCT BmyI 131 GGGCTC G[AGT]GC[ACT]^C BsgI 928 GTGCAG GTGCAG(16/14) BsiEI 1043 CGGCCG CG[GA][CT]^CG Bsp1286I 131 GGGCTC G[AGT]GC[ACT]^C BspMI 1766 GCAGGT ACCTGC(4/8) BsrDI 1825 GCAATG GCAATG(2/0) BstAPI 688 GCACCTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CfoI 388 GCGC GCG^C DraI 1957 TTTAAA TTT^AAA DraII 663 AGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 689 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1039 GACTCGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 81 CCATGG C^C[GA][CT]GG EagI 1043 CGGCCG C^GGCCG EclXI 1043 CGGCCG C^GGCCG Eco52I 1043 CGGCCG C^GGCCG EcoO109I 663 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoT14I 81 CCATGG C^C[AT][AT]GG EcoT22I 1790 ATGCAT ATGCA^T FbaI 1443 TGATCA T^GATCA Fnu4HI 1403 GCAGC GC^[ACGT]GC HapII 129 CCGG C^CGG HhaI 388 GCGC GCG^C HinP1I 388 GCGC G^CGC HincII 1855 GTTGAC GT[CT]^[GA]AC HindII 1855 GTTGAC GT[CT]^[GA]AC HpaII 129 CCGG C^CGG ItaI 1403 GCAGC GC^[ACGT]GC MluNI 78 TGGCCA TGG^CCA MscI 78 TGGCCA TGG^CCA MspI 129 CCGG C^CGG NciI 129 CCGGG CC^[GC]GG NcoI 81 CCATGG C^CATGG NheI 697 GCTAGC G^CTAGC NsiI 1790 ATGCAT ATGCA^T PacI 301 TTAATTAA TTAAT^TAA PshBI 1110 ATTAAT AT^TAAT SapI 177 GCTCTTC GCTCTTC(1/4) SmlI 1375 CTTGAG C^T[CT][GA]AG StyI 81 CCATGG C^C[AT][AT]GG TaqI 1282 TCGA T^CGA TseI 1403 GCAGC G^C[AT]GC TthHB8I 1282 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwNI 662 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG 1259 CAGCTACTG BbsI 115 GAAGAC GAAGAC(2/6) 1695 GTCTTC BpmI 1029 CTCCAG CTGGAG(16/14) 1874 CTCCAG BpuAI 115 GAAGAC GAAGAC(2/6) 1695 GTCTTC BsmAI 616 GAGAC GTCTC(1/5) 1530 GAGAC BstXI 468 CCAAGATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1909 CCAATGACCTGG BstYI 227 AGATCT [GA]^GATC[CT] 1725 AGATCC Cac8I 697 GCTAGC GC[ACGT]^[ACGT]GC 1523 GCTTGC EaeI 78 TGGCCA [CT]^GGCC[GA] 1043 CGGCCG EcoNI 1354 CCTGATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1873 CCTCCAGTAGG HindIII 1521 AAGCTT A^AGCTT 1744 AAGCTT HphI 427 GGTGA GGTGA(8/7) 1312 GGTGA MflI 227 AGATCT [GA]^GATC[CT] 1725 AGATCC MwoI 688 GCACCTAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 930 GCAGAATCAGC NdeI 901 CATATG CA^TATG 1505 CATATG NlaIV 1065 GGTCCC GG[ACGT]^[ACGT]CC 1882 GGGACC ScaI 38 AGTACT AGT^ACT 1981 AGTACT SfaNI 1122 GATGC GCATC(5/9) 374 GCATC SfcI 26 CTACAG C^T[GA][CT]AG 255 CTATAG SspI 142 AATATT AAT^ATT 835 AATATT Tsp45I 360 GTCAC ^GT[GC]AC 428 GTGAC XhoII 227 AGATCT [GA]^GATC[CT] 1725 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI AvaII BfaI BsaJI BseRI BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EarI EcoRII FokI HaeIII HinfI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MvaI NdeII NlaIII PleI RsaI Sau3AI Sau96I ScrFI TfiI Tru9I Tsp509I TspRI