Restriction map for
pF1KB9510
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AvaI AviII
BalI BamHI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI
BglII BmyI BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstF5I BstPI BstUI BstZ17I
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FokI FseI
FspI HaeII HgaI Hin1I HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Van91I XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AflII 805 CTTAAG C^TTAAG
AocI 451 CCTAAGG CC^T[ACGT]AGG
AspI 931 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 133 CCTAGG C^CTAGG
BanI 1039 GGTGCC G^G[CT][GA]CC
BbrPI 108 CACGTG CAC^GTG
BcnI 627 CCGGG CC^[GC]GG
BfrI 805 CTTAAG C^TTAAG
BlnI 133 CCTAGG C^CTAGG
BspMI 393 ACCTGC ACCTGC(4/8)
BsrDI 775 GCAATG GCAATG(2/0)
BstAPI 964 GCAGCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 172 CCAGCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 194 GGATCT [GA]^GATC[CT]
Bsu36I 451 CCTAAGG CC^T[ACGT]AGG
CfoI 290 GCGC GCG^C
DraII 356 AGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 545 CTCTTC CTCTTC(1/4)
Eco81I 451 CCTAAGG CC^T[ACGT]AGG
EcoNI 78 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 356 AGGTCCT [GA]G^G[ACGT]CC[CT]
HaeIII 1113 GGCC GG^CC
HapII 627 CCGG C^CGG
HhaI 290 GCGC GCG^C
HinP1I 290 GCGC G^CGC
HindIII 1089 AAGCTT A^AGCTT
HpaII 627 CCGG C^CGG
Ksp632I 545 CTCTTC CTCTTC(1/4)
MflI 194 GGATCT [GA]^GATC[CT]
MspI 627 CCGG C^CGG
NciI 627 CCGGG CC^[GC]GG
PflFI 931 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 108 CACGTG CAC^GTG
PmlI 108 CACGTG CAC^GTG
PpuMI 356 AGGTCCT [GA]G^G[AT]CC[CT]
ScaI 1015 AGTACT AGT^ACT
SexAI 44 ACCAGGT A^CC[AT]GGT
SfaNI 538 GATGC GCATC(5/9)
SmlI 805 CTTAAG C^T[CT][GA]AG
TaqI 514 TCGA T^CGA
Tth111I 931 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 514 TCGA T^CGA
XbaI 492 TCTAGA T^CTAGA
XcmI 263 CCAAAATCAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 194 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwNI 398 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
827 CAGAACCTG
BfaI 134 CTAG C^TAG
493 CTAG
BlpI 66 GCTAAGC GC^T[ACGT]AGC
340 GCTCAGC
BpmI 680 CTCCAG CTGGAG(16/14)
1098 CTCCAG
Bpu1102I 66 GCTAAGC GC^T[ACGT]AGC
340 GCTCAGC
BsaAI 108 CACGTG [CT]AC^GT[GA]
757 CACGTA
BsiYI 78 CCTGCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
611 CCAGTGTTGGG
BslI 78 CCTGCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
611 CCAGTGTTGGG
CelII 66 GCTAAGC GC^T[ACGT]AGC
340 GCTCAGC
EcoT14I 133 CCTAGG C^C[AT][AT]GG
482 CCTTGG
HincII 3 GTCAAC GT[CT]^[GA]AC
477 GTTGAC
HindII 3 GTCAAC GT[CT]^[GA]AC
477 GTTGAC
HinfI 168 GAGTC G^A[ACGT]TC
516 GAGTC
MaeI 134 CTAG C^TAG
493 CTAG
MaeII 109 ACGT A^CGT
758 ACGT
NlaIV 41 GGAACC GG[ACGT]^[ACGT]CC
1039 GGTGCC
PleI 168 GAGTC GAGTC(4/5)
516 GAGTC
StyI 133 CCTAGG C^C[AT][AT]GG
482 CCTTGG
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BsaJI BsmFI BsrI BssKI
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI HphI ItaI
MaeIII MboI MboII MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfcI Tru9I TseI
Tsp45I Tsp509I TspRI