Restriction map for
pF1KB9521
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AvrII
BbrPI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiWI BsmI
Bsp1407I BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I CelII Cfr10I
CpoI DraI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
MamI MfeI MluI MroI MseI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SspBI SspI SwaI
TfiI Tru9I Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AspHI 933 GTGCTC G[AT]GC[AT]^C
AviII 1001 TGCGCA TGC^GCA
BamHI 1720 GGATCC G^GATCC
BbeI 900 GGCGCC GGCGC^C
BbsI 274 GTCTTC GAAGAC(2/6)
BfaI 163 CTAG C^TAG
BglI 1164 GCCTGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 142 CTGGAG CTGGAG(16/14)
BpuAI 274 GTCTTC GAAGAC(2/6)
BsiEI 320 CGGTCG CG[GA][CT]^CG
BsiHKAI 933 GTGCTC G[AT]GC[AT]^C
BsmBI 1671 GAGACG CGTCTC(1/5)
BspDI 1525 ATCGAT AT^CGAT
BsrDI 1017 GCAATG GCAATG(2/0)
BstEII 804 GGTCACC G^GT[ACGT]ACC
BstPI 804 GGTCACC G^GT[ACGT]ACC
BstYI 1720 GGATCC [GA]^GATC[CT]
ClaI 1525 ATCGAT AT^CGAT
DraIII 1005 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1312 GACATCCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 804 GGTCACC G^GT[ACGT]ACC
FspI 1001 TGCGCA TGC^GCA
HincII 367 GTCAAC GT[CT]^[GA]AC
HindII 367 GTCAAC GT[CT]^[GA]AC
KasI 900 GGCGCC G^GCGCC
KspI 495 CCGCGG CCGC^GG
MaeI 163 CTAG C^TAG
MaeII 998 ACGT A^CGT
MflI 1720 GGATCC [GA]^GATC[CT]
NarI 900 GGCGCC GG^CGCC
NcoI 938 CCATGG C^CATGG
PstI 799 CTGCAG CTGCA^G
SacII 495 CCGCGG CCGC^GG
SapI 1179 GCTCTTC GCTCTTC(1/4)
SbfI 798 CCTGCAGG CCTGCA^GG
SfcI 799 CTGCAG C^T[GA][CT]AG
SfoI 900 GGCGCC GGC^GCC
SphI 405 GCATGC GCATG^C
Sse8387I 798 CCTGCAGG CCTGCA^GG
StuI 29 AGGCCT AGG^CCT
TaqI 1526 TCGA T^CGA
TthHB8I 1526 TCGA T^CGA
XcmI 203 CCACCTTTGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1720 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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Acc65I 700 GGTACC G^GTACC
720 GGTACC
AccII 496 CGCG CG^CG
991 CGCG
AcyI 900 GGCGCC G[GA]^CG[CT]C
1229 GGCGTC
Aor51HI 482 AGCGCT AGC^GCT
983 AGCGCT
Asp718I 700 GGTACC G^GTACC
720 GGTACC
BbvCI 105 CCTCAGC CCTCAGC(-5/-2)
378 CCTCAGC
BsaHI 900 GGCGCC G[GA]^CG[CT]C
1229 GGCGTC
BsgI 1288 CTGCAC GTGCAG(16/14)
217 GTGCAG
BspMI 310 ACCTGC ACCTGC(4/8)
801 GCAGGT
BstAPI 396 GCAGGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
400 GCACTGCATGC
BstUI 496 CGCG CG^CG
991 CGCG
BstXI 728 CCATTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
860 CCATCTTGGTGG
EarI 1180 CTCTTC CTCTTC(1/4)
1405 GAAGAG
Eco47III 482 AGCGCT AGC^GCT
983 AGCGCT
Hin1I 900 GGCGCC G[GA]^CG[CT]C
1229 GGCGTC
KpnI 700 GGTACC GGTAC^C
720 GGTACC
Ksp632I 1180 CTCTTC CTCTTC(1/4)
1405 GAAGAG
MaeIII 805 GTCAC ^GT[ACGT]AC
1067 GTGAC
MvnI 496 CGCG CG^CG
991 CGCG
SmlI 886 CTCAAG C^T[CT][GA]AG
1192 CTTGAG
Tsp45I 805 GTCAC ^GT[GC]AC
1067 GTGAC
Tsp509I 570 AATT ^AATT
1442 AATT
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AvaI AvaII BalI BanI
BanII BbvI BcgI BcnI BmyI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TseI TspRI Van91I