##################################
# NO CUT ENZYMES ( 184 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaII
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BbvI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsaJI BsaWI BseAI BsgI
BsiWI BsmBI BsmFI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DdeI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
Fnu4HI FseI FspI HapII HgaI
Hin1I HincII HindII HindIII HpaI
HpaII ItaI KasI KpnI KspI
MaeII MfeI MluI MluNI MroI
MscI MseI MspA1I MspI MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tru9I TseI Tsp45I
TspRI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 190 CGCG CG^CG
AfaI 177 GTAC GT^AC
Aor51HI 207 AGCGCT AGC^GCT
AspHI 364 GAGCTC G[AT]GC[AT]^C
AvaI 203 CCCGAG C^[CT]CG[GA]G
BanI 199 GGCACC G^G[CT][GA]CC
BbsI 346 GAAGAC GAAGAC(2/6)
BfaI 64 CTAG C^TAG
BpuAI 346 GAAGAC GAAGAC(2/6)
BsaBI 303 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 49 GAGGAG GAGGAG(10/8)
BsiEI 130 CGACCG CG[GA][CT]^CG
BsiHKAI 364 GAGCTC G[AT]GC[AT]^C
BsmAI 256 GAGAC GTCTC(1/5)
BsmI 78 GAATGC GAATGC(1/-1)
BsoBI 203 CCCGAG C^[CT]CG[GA]G
BspMI 326 ACCTGC ACCTGC(4/8)
BsrDI 273 CATTGC GCAATG(2/0)
BsrI 350 ACTGG ACTGG(1/-1)
BssKI 96 CCAGG ^CC[ACGT]GG
BstF5I 54 GGATG GGATG(2/0)
BstNI 96 CCAGG CC^[AT]GG
BstUI 190 CGCG CG^CG
CfoI 208 GCGC GCG^C
Cfr13I 294 GGCCC G^G[ACGT]CC
EarI 85 GAAGAG CTCTTC(1/4)
Eco47III 207 AGCGCT AGC^GCT
EcoRII 96 CCAGG ^CC[AT]GG
FauI 188 CCCGC CCCGC(4/6)
FokI 54 GGATG GGATG(9/13)
HaeII 207 AGCGCT [GA]GCGC^[CT]
HhaI 208 GCGC GCG^C
HinP1I 208 GCGC G^CGC
HinfI 152 GAATC G^A[ACGT]TC
Ksp632I 85 GAAGAG CTCTTC(1/4)
MaeI 64 CTAG C^TAG
MaeIII 323 GTTAC ^GT[ACGT]AC
MamI 303 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 273 CATTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 96 CCAGG CC^[AT]GG
MvnI 190 CGCG CG^CG
NlaIV 199 GGCACC GG[ACGT]^[ACGT]CC
RsaI 177 GTAC GT^AC
SacI 364 GAGCTC GAGCT^C
Sau96I 294 GGCCC G^G[ACGT]CC
ScrFI 96 CCAGG CC^[ACGT]GG
SmlI 289 CTCAAG C^T[CT][GA]AG
TaqI 107 TCGA T^CGA
TfiI 152 GAATC G^A[AT]TC
Tsp509I 19 AATT ^AATT
TthHB8I 107 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 7 enzymes) #
###################################
AciI 133 CCGC CCGC(-3/-1)
189 CCGC
BanII 185 GAGCCC G[GA]GC[CT]^C
364 GAGCTC
BpmI 211 CTCCAG CTGGAG(16/14)
247 CTGGAG
HphI 155 TCACC GGTGA(8/7)
374 TCACC
MboII 85 GAAGA GAAGA(8/7)
346 GAAGA
MwoI 191 GCGTGCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
220 GCGATGTGTGC
NlaIII 168 CATG CATG^
279 CATG
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AluI AlwI BmyI BsiYI BslI
Bsp1286I BstYI Cac8I DpnII HaeIII
MboI MflI MnlI NdeII Sau3AI
XhoII