################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI AlwNI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiWI BsmBI BspDI BspEI BspHI BsrBI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoT22I FbaI FseI HaeII Hin1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MroI MseI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tru9I Van91I XcmI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Alw44I 831 GTGCAC G^TGCAC AlwI 432 GATCC GGATC(4/5) ApaLI 831 GTGCAC G^TGCAC AspI 1347 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1320 TGCGCA TGC^GCA BfaI 55 CTAG C^TAG BsaI 844 GAGACC GGTCTC(1/5) BseRI 234 CTCCTC GAGGAG(10/8) BsiEI 417 CGACCG CG[GA][CT]^CG BsmI 524 GAATGC GAATGC(1/-1) Bsp1407I 1212 TGTACA T^GTACA BspMI 1232 ACCTGC ACCTGC(4/8) BsrDI 920 CATTGC GCAATG(2/0) BsrFI 46 GCCGGC [GA]^CCGG[CT] BsrGI 1212 TGTACA T^GTACA BssSI 1019 CTCGTG CACGAG(-5/-1) BstAPI 1323 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 767 CCAAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 46 GCCGGC [GA]^CCGG[CT] EcoT14I 872 CCAAGG C^C[AT][AT]GG FspI 1320 TGCGCA TGC^GCA HgaI 756 GACGC GACGC(5/10) HindIII 141 AAGCTT A^AGCTT MaeI 55 CTAG C^TAG MluI 350 ACGCGT A^CGCGT NaeI 46 GCCGGC GCC^GGC NgoMIV 46 GCCGGC G^CCGGC PaeR7I 40 CTCGAG C^TCGAG PflFI 1347 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC SacI 856 GAGCTC GAGCT^C SmaI 674 CCCGGG CCC^GGG SphI 1317 GCATGC GCATG^C SspBI 1212 TGTACA T^GTACA StyI 872 CCAAGG C^C[AT][AT]GG Tth111I 1347 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 54 TCTAGA T^CTAGA XhoI 40 CTCGAG C^TCGAG XmaCI 674 CCCGGG C^CCGGG XmaI 674 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 351 CGCG CG^CG 507 CGCG AfaI 1213 GTAC GT^AC 1401 GTAC AocI 326 CCTCAGG CC^T[ACGT]AGG 1038 CCTAAGG Asp700I 645 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1044 GAAACCGTTC AvaI 40 CTCGAG C^[CT]CG[GA]G 674 CCCGGG BsgI 1366 CTGCAC GTGCAG(16/14) 1330 GTGCAG BsoBI 40 CTCGAG C^[CT]CG[GA]G 674 CCCGGG BspLU11I 1215 ACATGT A^CATGT 1397 ACATGT BstUI 351 CGCG CG^CG 507 CGCG Bsu36I 326 CCTCAGG CC^T[ACGT]AGG 1038 CCTAAGG Cac8I 46 GCCGGC GC[ACGT]^[ACGT]GC 1317 GCATGC CfoI 1321 GCGC GCG^C 1363 GCGC EarI 1264 CTCTTC CTCTTC(1/4) 73 GAAGAG Eco81I 326 CCTCAGG CC^T[ACGT]AGG 1038 CCTAAGG EcoRI 865 GAATTC G^AATTC 1024 GAATTC FauI 1371 CCCGC CCCGC(4/6) 314 GCGGG HhaI 1321 GCGC GCG^C 1363 GCGC HinP1I 1321 GCGC G^CGC 1363 GCGC Ksp632I 1264 CTCTTC CTCTTC(1/4) 73 GAAGAG MaeII 835 ACGT A^CGT 1178 ACGT MslI 349 CACGCGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 834 CACGTCAGTG MvnI 351 CGCG CG^CG 507 CGCG NlaIV 255 GGTCCC GG[ACGT]^[ACGT]CC 1065 GGAACC RsaI 1213 GTAC GT^AC 1401 GTAC SmlI 40 CTCGAG C^T[CT][GA]AG 394 CTCAAG StuI 323 AGGCCT AGG^CCT 635 AGGCCT XmnI 645 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1044 GAAACCGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AcsI AflIII AluI ApoI AspHI AvaII BalI BanII BbsI BbvI BcnI BglII BmyI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EaeI EcoRII EcoRV Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NspI PleI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII