################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII AccI AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BclI BfrI BglII BlnI BsaAI BsaI BseRI BsgI BsiEI BsiWI BsmI BspHI BspMI BsrBI BsrDI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Cfr10I CpoI DraI DraII DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KspI MfeI MluI MluNI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tsp509I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 393 GGTACC G^GTACC AccIII 1019 TCCGGA T^CCGGA AflIII 845 ACATGT A^C[GA][CT]GT AocI 357 CCTCAGG CC^T[ACGT]AGG Asp718I 393 GGTACC G^GTACC AspHI 891 GTGCTC G[AT]GC[AT]^C AvaI 547 CCCGAG C^[CT]CG[GA]G BanII 343 GAGCCC G[GA]GC[CT]^C BbsI 969 GAAGAC GAAGAC(2/6) BbvI 597 GCTGC GCAGC(8/12) BcgI 20 GCAAGACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 238 GGATAC GTATCC(6/5) BglI 1135 GCCAACACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 969 GAAGAC GAAGAC(2/6) BsaBI 1011 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1019 TCCGGA [AT]^CCGG[AT] BseAI 1019 TCCGGA T^CCGGA BsiHKAI 891 GTGCTC G[AT]GC[AT]^C BsmBI 602 GAGACG CGTCTC(1/5) BsoBI 547 CCCGAG C^[CT]CG[GA]G Bsp1407I 128 TGTACA T^GTACA BspDI 583 ATCGAT AT^CGAT BspEI 1019 TCCGGA T^CCGGA BspLU11I 845 ACATGT A^CATGT BsrGI 128 TGTACA T^GTACA BssSI 1113 CACGAG CACGAG(-5/-1) BstAPI 591 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 563 CCATCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1091 AGATCC [GA]^GATC[CT] Bsu36I 357 CCTCAGG CC^T[ACGT]AGG ClaI 583 ATCGAT AT^CGAT DrdI 775 GACCAGCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 746 CCACGG C^C[GA][CT]GG Eco81I 357 CCTCAGG CC^T[ACGT]AGG EcoNI 353 CCTACCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG Fnu4HI 597 GCTGC GC^[ACGT]GC HaeII 12 GGCGCT [GA]GCGC^[CT] ItaI 597 GCTGC GC^[ACGT]GC KpnI 393 GGTACC GGTAC^C MaeII 694 ACGT A^CGT MamI 1011 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1091 AGATCC [GA]^GATC[CT] MroI 1019 TCCGGA T^CCGGA MseI 271 TTAA T^TAA NspI 845 ACATGT [GA]CATG^[CT] SspBI 128 TGTACA T^GTACA TfiI 294 GATTC G^A[AT]TC Tru9I 271 TTAA T^TAA TseI 597 GCTGC G^C[AT]GC XhoII 1091 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccII 781 CGCG CG^CG 1096 CGCG AlwNI 592 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1070 CAGACGCTG Asp700I 289 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 812 GAAACATTTC BanI 393 GGTACC G^G[CT][GA]CC 1046 GGTGCC BcnI 945 CCGGG CC^[GC]GG 1146 CCCGG BfaI 160 CTAG C^TAG 250 CTAG BlpI 15 GCTGAGC GC^T[ACGT]AGC 954 GCTGAGC Bpu1102I 15 GCTGAGC GC^T[ACGT]AGC 954 GCTGAGC BstUI 781 CGCG CG^CG 1096 CGCG Cac8I 702 GCCAGC GC[ACGT]^[ACGT]GC 959 GCTGGC CelII 15 GCTGAGC GC^T[ACGT]AGC 954 GCTGAGC CfoI 13 GCGC GCG^C 780 GCGC FauI 687 CCCGC CCCGC(4/6) 911 GCGGG HhaI 13 GCGC GCG^C 780 GCGC HinP1I 13 GCGC G^CGC 780 GCGC MaeI 160 CTAG C^TAG 250 CTAG MvnI 781 CGCG CG^CG 1096 CGCG NciI 945 CCGGG CC^[GC]GG 1146 CCCGG TaqI 584 TCGA T^CGA 734 TCGA TthHB8I 584 TCGA T^CGA 734 TCGA XmnI 289 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 812 GAAACATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AvaII BmyI BpmI BsaHI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EarI EcoRII FokI HaeIII HapII HgaI Hin1I HinfI HpaII HphI Ksp632I MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI Tsp45I TspRI