Restriction map for
pF1KB9554
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaII AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiWI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DpnII DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII HphI
KasI KpnI KspI MaeII MamI
MboI MfeI MflI MluI MroI
MseI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI Sau3AI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI StuI StyI SwaI TaqI
TfiI Tru9I Tsp509I Tth111I TthHB8I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AvaI 316 CCCGAG C^[CT]CG[GA]G
BalI 427 TGGCCA TGG^CCA
BanI 462 GGCACC G^G[CT][GA]CC
BbsI 406 GTCTTC GAAGAC(2/6)
BfaI 298 CTAG C^TAG
BpuAI 406 GTCTTC GAAGAC(2/6)
BsaI 239 GAGACC GGTCTC(1/5)
BsiEI 30 CGACCG CG[GA][CT]^CG
BsmAI 239 GAGAC GTCTC(1/5)
BsoBI 316 CCCGAG C^[CT]CG[GA]G
BspMI 242 ACCTGC ACCTGC(4/8)
BsrBI 33 CCGCTC CCGCTC(-3/-3)
BstXI 137 CCATCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 419 CCGTGG C^C[GA][CT]GG
EaeI 427 TGGCCA [CT]^GGCC[GA]
EarI 345 GAAGAG CTCTTC(1/4)
FauI 459 GCGGG CCCGC(4/6)
HinfI 497 GAGTC G^A[ACGT]TC
Ksp632I 345 GAAGAG CTCTTC(1/4)
MaeI 298 CTAG C^TAG
MaeIII 20 GTGAC ^GT[ACGT]AC
MluNI 427 TGGCCA TGG^CCA
MscI 427 TGGCCA TGG^CCA
MslI 258 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 131 CAGCTG C[AC]G^C[GT]G
PflMI 284 CCAGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 497 GAGTC GAGTC(4/5)
PvuII 131 CAGCTG CAG^CTG
SmlI 91 CTCAAG C^T[CT][GA]AG
Tsp45I 20 GTGAC ^GT[GC]AC
Van91I 284 CCAGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 111 GTAC GT^AC
414 GTAC
AflIII 116 ACATGT A^C[GA][CT]GT
227 ACATGT
AlwNI 247 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
439 CAGGAGCTG
AspHI 310 GAGCTC G[AT]GC[AT]^C
363 GAGCTC
BanII 310 GAGCTC G[GA]GC[CT]^C
363 GAGCTC
BpmI 328 CTGGAG CTGGAG(16/14)
340 CTGGAG
BseRI 268 GAGGAG GAGGAG(10/8)
271 GAGGAG
BsiHKAI 310 GAGCTC G[AT]GC[AT]^C
363 GAGCTC
BspLU11I 116 ACATGT A^CATGT
227 ACATGT
Cfr13I 164 GGCCC G^G[ACGT]CC
321 GGGCC
MboII 345 GAAGA GAAGA(8/7)
407 TCTTC
NlaIII 117 CATG CATG^
228 CATG
NlaIV 205 GGCTCC GG[ACGT]^[ACGT]CC
462 GGCACC
NspI 116 ACATGT [GA]CATG^[CT]
227 ACATGT
RsaI 111 GTAC GT^AC
414 GTAC
SacI 310 GAGCTC GAGCT^C
363 GAGCTC
Sau96I 164 GGCCC G^G[ACGT]CC
321 GGGCC
SbfI 167 CCTGCAGG CCTGCA^GG
243 CCTGCAGG
Sse8387I 167 CCTGCAGG CCTGCA^GG
243 CCTGCAGG
XcmI 192 CCAGTGCGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
469 CCACCTGGGGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AluI BbvI BlpI BmyI
Bpu1102I BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CelII DdeI EcoRII Fnu4HI FokI
HaeIII ItaI MnlI MvaI MwoI
PstI ScrFI SfaNI SfcI TseI
TspRI