################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BbeI BbrPI BbsI BbvI BclI BfaI BfrI BglI BglII BlnI BpuAI BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I ClaI CpoI DraI DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SbfI ScaI SfcI SfiI SfoI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tru9I TseI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 22 enzymes) # ################################### AflIII 1256 ACGTGT A^C[GA][CT]GT BamHI 899 GGATCC G^GATCC BbvCI 300 CCTCAGC CCTCAGC(-5/-2) BcgI 1296 GCACCTGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 233 GTATCC GTATCC(6/5) BlpI 623 GCTCAGC GC^T[ACGT]AGC Bpu1102I 623 GCTCAGC GC^T[ACGT]AGC BsaAI 1255 TACGTG [CT]AC^GT[GA] BseRI 1044 CTCCTC GAGGAG(10/8) BsmI 682 GAATGC GAATGC(1/-1) BstXI 1014 CCAGAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 623 GCTCAGC GC^T[ACGT]AGC DraIII 472 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRI 731 GAATTC G^AATTC Fnu4HI 1271 GCGGC GC^[ACGT]GC ItaI 1271 GCGGC GC^[ACGT]GC MaeII 1256 ACGT A^CGT MspA1I 895 CAGCGG C[AC]G^C[GT]G NspI 305 GCATGT [GA]CATG^[CT] SexAI 473 ACCTGGT A^CC[AT]GGT SgrAI 1074 CACCGGTG C[GA]^CCGG[CT]G SmlI 210 CTCAAG C^T[CT][GA]AG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 195 GAATTT [GA]^AATT[CT] 731 GAATTC AgeI 1075 ACCGGT A^CCGGT 1205 ACCGGT ApoI 195 GAATTT [GA]^AATT[CT] 731 GAATTC BanI 554 GGCACC G^G[CT][GA]CC 1202 GGCACC BpmI 286 CTCCAG CTGGAG(16/14) 708 CTCCAG BsaI 31 GAGACC GGTCTC(1/5) 747 GAGACC BsgI 350 GTGCAG GTGCAG(16/14) 675 GTGCAG BsmAI 31 GAGAC GTCTC(1/5) 747 GAGAC BsmFI 95 GGGAC GGGAC(10/14) 368 GGGAC BssSI 724 CACGAG CACGAG(-5/-1) 838 CACGAG BstAPI 677 GCAGGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1262 GCAATCTGTGC CfoI 1065 GCGC GCG^C 1132 GCGC EarI 268 CTCTTC CTCTTC(1/4) 328 GAAGAG HhaI 1065 GCGC GCG^C 1132 GCGC HinP1I 1065 GCGC G^CGC 1132 GCGC Ksp632I 268 CTCTTC CTCTTC(1/4) 328 GAAGAG MslI 920 CATACAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1072 CACACCGGTG NlaIII 306 CATG CATG^ 341 CATG PinAI 1075 ACCGGT A^CCGGT 1205 ACCGGT PleI 370 GACTC GAGTC(4/5) 58 GAGTC SapI 328 GAAGAGC GCTCTTC(1/4) 267 GCTCTTC TaqI 426 TCGA T^CGA 1220 TCGA Tsp509I 196 AATT ^AATT 732 AATT TthHB8I 426 TCGA T^CGA 1220 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AluI AlwI Asp700I AspHI AvaII BanII BcnI BmyI BsaJI BsaWI BsiHKAI BsiYI BslI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr10I Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI FokI HaeIII HapII HinfI HpaII HphI MaeIII MboI MboII MflI MnlI MspI MvaI MwoI NciI NdeII NlaIV PpuMI SacI Sau3AI Sau96I ScrFI SfaNI StuI TfiI Tsp45I TspRI XhoII XmnI