Restriction map for
pF1KB9564
##################################
# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HincII
HindII HpaI KpnI KspI MaeII
MaeIII MamI MfeI MluI MroI
MseI MunI MvnI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfcI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp45I
Tsp509I Tth111I Van91I XbaI XcmI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 421 GTCTAC GT^[AC][GT]AC
AcyI 683 GGCGCC G[GA]^CG[CT]C
BalI 375 TGGCCA TGG^CCA
BamHI 473 GGATCC G^GATCC
BbeI 683 GGCGCC GGCGC^C
BbsI 237 GTCTTC GAAGAC(2/6)
BpmI 9 CTCCAG CTGGAG(16/14)
BpuAI 237 GTCTTC GAAGAC(2/6)
BsaHI 683 GGCGCC G[GA]^CG[CT]C
BsaI 214 GAGACC GGTCTC(1/5)
BsmI 321 GAATGC GAATGC(1/-1)
BsrBI 695 GAGCGG CCGCTC(-3/-3)
BstF5I 195 GGATG GGATG(2/0)
BstYI 473 GGATCC [GA]^GATC[CT]
DraIII 223 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 25 CCATGG C^C[GA][CT]GG
EarI 135 GAAGAG CTCTTC(1/4)
EcoNI 348 CCTCGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 710 CCCGC CCCGC(4/6)
FokI 195 GGATG GGATG(9/13)
HaeII 683 GGCGCC [GA]GCGC^[CT]
Hin1I 683 GGCGCC G[GA]^CG[CT]C
HindIII 490 AAGCTT A^AGCTT
HinfI 235 GAGTC G^A[ACGT]TC
KasI 683 GGCGCC G^GCGCC
Ksp632I 135 GAAGAG CTCTTC(1/4)
MflI 473 GGATCC [GA]^GATC[CT]
MluNI 375 TGGCCA TGG^CCA
MscI 375 TGGCCA TGG^CCA
MspA1I 313 CAGCTG C[AC]G^C[GT]G
NarI 683 GGCGCC GG^CGCC
NcoI 25 CCATGG C^CATGG
NspI 36 GCATGT [GA]CATG^[CT]
PaeR7I 634 CTCGAG C^TCGAG
PleI 235 GAGTC GAGTC(4/5)
PvuII 313 CAGCTG CAG^CTG
SexAI 224 ACCTGGT A^CC[AT]GGT
SfiI 699 GGCCAAGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 683 GGCGCC GGC^GCC
SmaI 551 CCCGGG CCC^GGG
SmlI 634 CTCGAG C^T[CT][GA]AG
XhoI 634 CTCGAG C^TCGAG
XhoII 473 GGATCC [GA]^GATC[CT]
XmaCI 551 CCCGGG C^CCGGG
XmaI 551 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 313 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
355 CAGGGCCTG
AvaII 60 GGTCC G^G[AT]CC
381 GGTCC
BanI 534 GGTGCC G^G[CT][GA]CC
683 GGCGCC
BcnI 551 CCCGG CC^[GC]GG
552 CCGGG
BfaI 64 CTAG C^TAG
334 CTAG
BglI 619 GCCTCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
700 GCCAAGGGGGC
BlpI 366 GCTCAGC GC^T[ACGT]AGC
594 GCTGAGC
Bpu1102I 366 GCTCAGC GC^T[ACGT]AGC
594 GCTGAGC
BsrFI 508 GCCGGC [GA]^CCGG[CT]
599 GCCGGC
CelII 366 GCTCAGC GC^T[ACGT]AGC
594 GCTGAGC
CfoI 678 GCGC GCG^C
684 GCGC
Cfr10I 508 GCCGGC [GA]^CCGG[CT]
599 GCCGGC
HhaI 678 GCGC GCG^C
684 GCGC
HinP1I 678 GCGC G^CGC
684 GCGC
HphI 340 GGTGA GGTGA(8/7)
22 TCACC
MaeI 64 CTAG C^TAG
334 CTAG
MslI 51 CATTCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
273 CATTTCTGTG
NaeI 508 GCCGGC GCC^GGC
599 GCCGGC
NciI 551 CCCGG CC^[GC]GG
552 CCGGG
NgoMIV 508 GCCGGC G^CCGGC
599 GCCGGC
SfaNI 196 GATGC GCATC(5/9)
14 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AlwI ApaI AvaI
BanII BbvI BmyI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstNI BstXI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
HaeIII HapII HpaII ItaI MboI
MboII MnlI MspI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI StyI TaqI TseI TspRI
TthHB8I