################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII Acc65I AccI AclI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AviII BbeI BbvCI BciVI BclI BglII Bsp1407I BsrDI BsrGI Bst1107I BstBI BstZ17I Bsu36I CpoI DraIII Eam1105I Eco47III Eco81I EcoRV EcoT22I FbaI FseI FspI KasI KpnI KspI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PmeI Psp1406I PvuI RsrII SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccIII 2570 TCCGGA T^CCGGA AflII 1674 CTTAAG C^TTAAG AgeI 2915 ACCGGT A^CCGGT Asp700I 3033 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 55 CCTAGG C^CTAGG BalI 2721 TGGCCA TGG^CCA BamHI 485 GGATCC G^GATCC BcgI 2973 CGAGGTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 1674 CTTAAG C^TTAAG BlnI 55 CCTAGG C^CTAGG BsaBI 2574 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 2570 TCCGGA T^CCGGA BsiWI 3324 CGTACG C^GTACG BsmI 2683 GAATGC GAATGC(1/-1) BspDI 3356 ATCGAT AT^CGAT BspEI 2570 TCCGGA T^CCGGA BspLU11I 1577 ACATGT A^CATGT BsrBI 1662 GAGCGG CCGCTC(-3/-3) BssHII 1366 GCGCGC G^CGCGC BstAPI 3540 GCAGTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2997 GGTGACC G^GT[ACGT]ACC BstPI 2997 GGTGACC G^GT[ACGT]ACC ClaI 3356 ATCGAT AT^CGAT DraI 1761 TTTAAA TTT^AAA EagI 3110 CGGCCG C^GGCCG EarI 735 GAAGAG CTCTTC(1/4) EclXI 3110 CGGCCG C^GGCCG Eco52I 3110 CGGCCG C^GGCCG EcoO65I 2997 GGTGACC G^GT[ACGT]ACC HaeII 714 GGCGCT [GA]GCGC^[CT] HincII 1410 GTTAAC GT[CT]^[GA]AC HindII 1410 GTTAAC GT[CT]^[GA]AC HpaI 1410 GTTAAC GTT^AAC Ksp632I 735 GAAGAG CTCTTC(1/4) MamI 2574 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2721 TGGCCA TGG^CCA MroI 2570 TCCGGA T^CCGGA MscI 2721 TGGCCA TGG^CCA NaeI 2460 GCCGGC GCC^GGC NgoMIV 2460 GCCGGC G^CCGGC PaeR7I 2971 CTCGAG C^TCGAG PflMI 960 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 2915 ACCGGT A^CCGGT PshAI 2278 GACTGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SbfI 2489 CCTGCAGG CCTGCA^GG Sse8387I 2489 CCTGCAGG CCTGCA^GG SspI 1039 AATATT AAT^ATT StuI 1729 AGGCCT AGG^CCT Van91I 960 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 2971 CTCGAG C^TCGAG XmnI 3033 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AseI 1042 ATTAAT AT^TAAT 1846 ATTAAT AsnI 1042 ATTAAT AT^TAAT 1846 ATTAAT AspHI 85 GAGCTC G[AT]GC[AT]^C 1279 GAGCTC BanI 2148 GGCACC G^G[CT][GA]CC 3591 GGTGCC BbrPI 498 CACGTG CAC^GTG 2644 CACGTG BbsI 307 GAAGAC GAAGAC(2/6) 2545 GAAGAC BglI 1200 GCCTGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2943 GCCCTCACGGC BlpI 3 GCTGAGC GC^T[ACGT]AGC 2095 GCTGAGC Bpu1102I 3 GCTGAGC GC^T[ACGT]AGC 2095 GCTGAGC BpuAI 307 GAAGAC GAAGAC(2/6) 2545 GAAGAC BsaI 2741 GAGACC GGTCTC(1/5) 3461 GAGACC BsgI 2437 CTGCAC GTGCAG(16/14) 3533 CTGCAC BsiEI 3110 CGGCCG CG[GA][CT]^CG 3320 CGACCG BsiHKAI 85 GAGCTC G[AT]GC[AT]^C 1279 GAGCTC BsmBI 743 GAGACG CGTCTC(1/5) 2537 GAGACG BspHI 759 TCATGA T^CATGA 1821 TCATGA BstXI 17 CCAAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1375 CCATTTTCTTGG BstYI 485 GGATCC [GA]^GATC[CT] 1689 GGATCT CelII 3 GCTGAGC GC^T[ACGT]AGC 2095 GCTGAGC DrdI 3184 GACATTGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3321 GACCGTACGGTC EcoNI 378 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3028 CCTAAGAAAGG EcoRI 1023 GAATTC G^AATTC 3230 GAATTC HindIII 1390 AAGCTT A^AGCTT 3419 AAGCTT MflI 485 GGATCC [GA]^GATC[CT] 1689 GGATCT PmaCI 498 CACGTG CAC^GTG 2644 CACGTG PmlI 498 CACGTG CAC^GTG 2644 CACGTG PshBI 1042 ATTAAT AT^TAAT 1846 ATTAAT PvuII 73 CAGCTG CAG^CTG 915 CAGCTG RcaI 759 TCATGA T^CATGA 1821 TCATGA SacI 85 GAGCTC GAGCT^C 1279 GAGCTC XcmI 2091 CCAAGCTGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2774 CCAGACTCGCCATGG XhoII 485 GGATCC [GA]^GATC[CT] 1689 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AspI AvaI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaAI BsaHI BsaJI BsaWI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I