##################################
# NO CUT ENZYMES (  169 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsiEI       BsiWI       BsmAI       
BsmBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrGI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DdeI        DpnII       DraI        DraIII      
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FokI        FseI        
FspI        HgaI        Hin1I       HincII      HindII      
HindIII     HinfI       HpaI        KasI        KpnI        
Ksp632I     MaeI        MaeIII      MamI        MboI        
MboII       MfeI        MflI        MluI        MroI        
MunI        NarI        NcoI        NdeII       NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsaI        RsrII       SacI        
SalI        SapI        Sau3AI      SbfI        ScaI        
SexAI       SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tsp45I      
Tsp509I     Tth111I     XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AspHI         160  GAGCAC               G[AT]GC[AT]^C
BanII         102  GAGCCC               G[GA]GC[CT]^C
BsgI           58  CTGCAC               GTGCAG(16/14)
BsiHKAI       160  GAGCAC               G[AT]GC[AT]^C
BsmI          189  GAATGC               GAATGC(1/-1)
BsrBI         198  GAGCGG               CCGCTC(-3/-3)
BsrDI         631  GCAATG               GCAATG(2/0)
BsrFI         330  GCCGGC               [GA]^CCGG[CT]
BssHII        254  GCGCGC               G^CGCGC
BssSI         195  CACGAG               CACGAG(-5/-1)
Cfr10I        330  GCCGGC               [GA]^CCGG[CT]
DraII         297  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoNI         418  CCTGCTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      297  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoT14I       689  CCTTGG               C^C[AT][AT]GG
HaeII          32  GGCGCT               [GA]GCGC^[CT]
KspI          266  CCGCGG               CCGC^GG
MaeII         620  ACGT                 A^CGT
MseI          278  TTAA                 T^TAA
MslI           81  CATGGGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          330  GCCGGC               GCC^GGC
NdeI           10  CATATG               CA^TATG
NgoMIV        330  GCCGGC               G^CCGGC
PpuMI         297  GGGTCCC              [GA]G^G[AT]CC[CT]
PvuII         343  CAGCTG               CAG^CTG
SacII         266  CCGCGG               CCGC^GG
SfiI          261  GGCCCCCGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StyI          689  CCTTGG               C^C[AT][AT]GG
TaqI          668  TCGA                 T^CGA
Tru9I         278  TTAA                 T^TAA
TspRI         657  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       668  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AvaII         298  GGTCC                G^G[AT]CC
              711  GGACC               
BalI           17  TGGCCA               TGG^CCA
              308  TGGCCA              
BanI           46  GGCACC               G^G[CT][GA]CC
              242  GGCACC              
BsrI          288  CCAGT                ACTGG(1/-1)
              363  CCAGT               
BstNI         272  CCAGG                CC^[AT]GG
              315  CCAGG               
EcoRII        272  CCAGG                ^CC[AT]GG
              315  CCAGG               
MluNI          17  TGGCCA               TGG^CCA
              308  TGGCCA              
MscI           17  TGGCCA               TGG^CCA
              308  TGGCCA              
MvaI          272  CCAGG                CC^[AT]GG
              315  CCAGG               
PstI          346  CTGCAG               CTGCA^G
              422  CTGCAG              
SfaNI         203  GCATC                GCATC(5/9)
              716  GCATC               
SfcI          346  CTGCAG               C^T[GA][CT]AG
              422  CTGCAG              
SphI          210  GCATGC               GCATG^C
              338  GCATGC              


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AccII       AciI        AluI        AvaI        BbvI        
BcnI        BglI        BmyI        BsaJI       BsiYI       
BslI        BsmFI       BsoBI       Bsp1286I    BssKI       
BstUI       Cac8I       CfoI        Cfr13I      DsaI        
EaeI        FauI        Fnu4HI      HaeIII      HapII       
HhaI        HinP1I      HpaII       HphI        ItaI        
MnlI        MspA1I      MspI        MvnI        MwoI        
NciI        NlaIII      NlaIV       NspI        PflMI       
Sau96I      ScrFI       TseI        Van91I