Restriction map for
pF1KB9603
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII BalI BbrPI BbsI BbvCI
BciVI BclI BfrI BglII BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BsgI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DdeI DraI DraIII
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI MaeII MamI MfeI MluI
MluNI MroI MscI MseI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SalI ScaI SexAI SfuI SmlI
SnaBI SpeI SphI SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AflIII 392 ACATGT A^C[GA][CT]GT
AlwNI 360 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 759 GAGCTC G[AT]GC[AT]^C
AspI 630 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 843 CCTAGG C^CTAGG
BamHI 977 GGATCC G^GATCC
BcgI 526 GCAGGGGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 843 CCTAGG C^CTAGG
BpmI 903 CTCCAG CTGGAG(16/14)
BseRI 900 CTCCTC GAGGAG(10/8)
BsiHKAI 759 GAGCTC G[AT]GC[AT]^C
BsmBI 478 GAGACG CGTCTC(1/5)
BspLU11I 392 ACATGT A^CATGT
BspMI 1005 GCAGGT ACCTGC(4/8)
BsrBI 519 GAGCGG CCGCTC(-3/-3)
BssHII 700 GCGCGC G^CGCGC
BstYI 977 GGATCC [GA]^GATC[CT]
HinfI 3 GAGTC G^A[ACGT]TC
MflI 977 GGATCC [GA]^GATC[CT]
MslI 171 CATCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 606 GCCGGC GCC^GGC
NcoI 176 CCATGG C^CATGG
NgoMIV 606 GCCGGC G^CCGGC
PflFI 630 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 3 GAGTC GAGTC(4/5)
PpuMI 721 GGGTCCC [GA]G^G[AT]CC[CT]
SacI 759 GAGCTC GAGCT^C
SapI 756 GAAGAGC GCTCTTC(1/4)
SfiI 450 GGCCCCGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 605 CGCCGGCG C[GA]^CCGG[CT]G
TspRI 619 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 630 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 977 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 416 GGCGCC G[GA]^CG[CT]C
609 GGCGCC
AfaI 60 GTAC GT^AC
1021 GTAC
BbeI 416 GGCGCC GGCGC^C
609 GGCGCC
BfaI 112 CTAG C^TAG
844 CTAG
BglI 97 GCCCCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
451 GCCCCGGAGGC
BsaHI 416 GGCGCC G[GA]^CG[CT]C
609 GGCGCC
BsaI 72 GAGACC GGTCTC(1/5)
133 GAGACC
BstF5I 288 CATCC GGATG(2/0)
795 CATCC
DrdI 313 GACTGCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
943 GACCCGCATGTC
EcoT14I 176 CCATGG C^C[AT][AT]GG
843 CCTAGG
FokI 288 CATCC GGATG(9/13)
795 CATCC
Hin1I 416 GGCGCC G[GA]^CG[CT]C
609 GGCGCC
HphI 655 GGTGA GGTGA(8/7)
6 TCACC
KasI 416 GGCGCC G^GCGCC
609 GGCGCC
KspI 101 CCGCGG CCGC^GG
569 CCGCGG
MaeI 112 CTAG C^TAG
844 CTAG
NarI 416 GGCGCC GG^CGCC
609 GGCGCC
NspI 392 ACATGT [GA]CATG^[CT]
948 GCATGT
PstI 556 CTGCAG CTGCA^G
1003 CTGCAG
RsaI 60 GTAC GT^AC
1021 GTAC
SacII 101 CCGCGG CCGC^GG
569 CCGCGG
SbfI 555 CCTGCAGG CCTGCA^GG
1002 CCTGCAGG
SfoI 416 GGCGCC GGC^GCC
609 GGCGCC
SmaI 445 CCCGGG CCC^GGG
717 CCCGGG
Sse8387I 555 CCTGCAGG CCTGCA^GG
1002 CCTGCAGG
StyI 176 CCATGG C^C[AT][AT]GG
843 CCTAGG
TaqI 14 TCGA T^CGA
941 TCGA
TthHB8I 14 TCGA T^CGA
941 TCGA
XmaCI 445 CCCGGG C^CCGGG
717 CCCGGG
XmaI 445 CCCGGG C^CCGGG
717 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AluI AlwI ApaI
AvaI AvaII BanI BanII BbvI
BcnI BmyI BsaJI BsiEI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI HinP1I HpaII ItaI Ksp632I
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI SfcI TseI Tsp45I