Restriction map for
pF1KB9619
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI AlwI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BcgI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrGI BsrI BssSI Bst1107I BstBI
BstEII BstF5I BstPI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FokI
FseI FspI HindIII HpaI KpnI
MaeI MamI MfeI MflI MluI
MroI MseI MunI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshBI Psp1406I
PstI PvuI RsrII SalI SapI
SbfI SexAI SfcI SfiI SfuI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tru9I Tsp509I TspRI Tth111I Van91I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 514 GTCTAC GT^[AC][GT]AC
AflIII 467 ACGTGT A^C[GA][CT]GT
Alw44I 3 GTGCAC G^TGCAC
ApaLI 3 GTGCAC G^TGCAC
BalI 672 TGGCCA TGG^CCA
BbrPI 451 CACGTG CAC^GTG
BbvCI 552 CCTCAGC CCTCAGC(-5/-2)
BciVI 55 GGATAC GTATCC(6/5)
BclI 293 TGATCA T^GATCA
BglI 176 GCCCAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 451 CACGTG [CT]AC^GT[GA]
BsaWI 140 ACCGGA [AT]^CCGG[AT]
BsiEI 60 CGGCCG CG[GA][CT]^CG
BsmBI 233 CGTCTC CGTCTC(1/5)
BspHI 983 TCATGA T^CATGA
BspMI 957 GCAGGT ACCTGC(4/8)
BstAPI 729 GCAGACCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 779 CCATCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 553 CTCAG C^T[ACGT]AG
DraIII 846 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 409 GACGGCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 60 CGGCCG C^GGCCG
EarI 249 CTCTTC CTCTTC(1/4)
EclXI 60 CGGCCG C^GGCCG
Eco52I 60 CGGCCG C^GGCCG
FbaI 293 TGATCA T^GATCA
HincII 121 GTCAAC GT[CT]^[GA]AC
HindII 121 GTCAAC GT[CT]^[GA]AC
Ksp632I 249 CTCTTC CTCTTC(1/4)
MluNI 672 TGGCCA TGG^CCA
MscI 672 TGGCCA TGG^CCA
PmaCI 451 CACGTG CAC^GTG
PmlI 451 CACGTG CAC^GTG
PshAI 987 GACCCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 983 TCATGA T^CATGA
SacI 615 GAGCTC GAGCT^C
ScaI 323 AGTACT AGT^ACT
TaqI 931 TCGA T^CGA
TthHB8I 931 TCGA T^CGA
XcmI 464 CCAACGTGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AlwNI 569 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1009 CAGCTGCTG
AspHI 3 GTGCAC G[AT]GC[AT]^C
615 GAGCTC
AvaII 169 GGACC G^G[AT]CC
608 GGACC
BanI 589 GGCGCC G^G[CT][GA]CC
703 GGCGCC
BbeI 589 GGCGCC GGCGC^C
703 GGCGCC
BbsI 790 GAAGAC GAAGAC(2/6)
900 GAAGAC
BpmI 868 CTGGAG CTGGAG(16/14)
886 CTGGAG
BpuAI 790 GAAGAC GAAGAC(2/6)
900 GAAGAC
BseRI 946 CTCCTC GAGGAG(10/8)
388 GAGGAG
BsgI 436 CTGCAC GTGCAG(16/14)
359 GTGCAG
BsiHKAI 3 GTGCAC G[AT]GC[AT]^C
615 GAGCTC
BsmAI 146 GTCTC GTCTC(1/5)
234 GTCTC
BssHII 745 GCGCGC G^CGCGC
880 GCGCGC
DpnII 294 GATC ^GATC
433 GATC
DsaI 336 CCGCGG C^C[GA][CT]GG
699 CCGCGG
HinfI 37 GACTC G^A[ACGT]TC
144 GAGTC
HphI 897 GGTGA GGTGA(8/7)
383 TCACC
KasI 589 GGCGCC G^GCGCC
703 GGCGCC
KspI 336 CCGCGG CCGC^GG
699 CCGCGG
MaeIII 382 GTCAC ^GT[ACGT]AC
454 GTGAC
MboI 294 GATC ^GATC
433 GATC
NaeI 520 GCCGGC GCC^GGC
940 GCCGGC
NarI 589 GGCGCC GG^CGCC
703 GGCGCC
NdeII 294 GATC ^GATC
433 GATC
NgoMIV 520 GCCGGC G^CCGGC
940 GCCGGC
NlaIII 786 CATG CATG^
984 CATG
PleI 37 GACTC GAGTC(4/5)
144 GAGTC
PvuII 685 CAGCTG CAG^CTG
1009 CAGCTG
SacII 336 CCGCGG CCGC^GG
699 CCGCGG
Sau3AI 294 GATC ^GATC
433 GATC
SfaNI 803 GCATC GCATC(5/9)
875 GCATC
SfoI 589 GGCGCC GGC^GCC
703 GGCGCC
SmaI 315 CCCGGG CCC^GGG
505 CCCGGG
SmlI 238 CTCAAG C^T[CT][GA]AG
907 CTCAAG
Tsp45I 382 GTCAC ^GT[GC]AC
454 GTGAC
XmaCI 315 CCCGGG C^CCGGG
505 CCCGGG
XmaI 315 CCCGGG C^CCGGG
505 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
ApaI AvaI BanII BbvI BcnI
BmyI BsaHI BsaJI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrFI BssKI
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DraII EaeI EcoO109I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
ItaI MaeII MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIV RsaI Sau96I ScrFI
SgrAI TseI