Restriction map for
pF1KB9621
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AccIII AclI AcsI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaLI
ApoI AscI AspI AviII AvrII
BalI BamHI BbeI BbrPI BcgI
BciVI BclI BfrI BlnI BsaAI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspLU11I BspMI BsrDI
BsrFI BssHII Bst1107I BstBI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
EaeI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI FspI HpaI
KasI Ksp632I KspI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NcoI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SapI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 1795 GGTACC G^GTACC
ApaI 890 GGGCCC GGGCC^C
AseI 1786 ATTAAT AT^TAAT
AsnI 1786 ATTAAT AT^TAAT
Asp700I 614 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1795 GGTACC G^GTACC
AspHI 1844 GTGCTC G[AT]GC[AT]^C
BanII 890 GGGCCC G[GA]GC[CT]^C
BglI 142 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 645 AGATCT A^GATCT
BsaBI 1032 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 1844 GTGCTC G[AT]GC[AT]^C
BsmI 1363 GAATGC GAATGC(1/-1)
Bsp1407I 920 TGTACA T^GTACA
BsrGI 920 TGTACA T^GTACA
BssSI 1847 CTCGTG CACGAG(-5/-1)
BstAPI 1478 GCATGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 910 GGTGACC G^GT[ACGT]ACC
BstPI 910 GGTGACC G^GT[ACGT]ACC
DrdI 1588 GACATCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 48 CCGTGG C^C[GA][CT]GG
EcoO65I 910 GGTGACC G^GT[ACGT]ACC
EcoT14I 1025 CCAAGG C^C[AT][AT]GG
HaeII 425 AGCGCC [GA]GCGC^[CT]
HapII 9 CCGG C^CGG
HindIII 559 AAGCTT A^AGCTT
HpaII 9 CCGG C^CGG
KpnI 1795 GGTACC GGTAC^C
MamI 1032 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 9 CCGG C^CGG
NheI 264 GCTAGC G^CTAGC
PpuMI 762 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 1786 ATTAAT AT^TAAT
SalI 376 GTCGAC G^TCGAC
SexAI 1872 ACCTGGT A^CC[AT]GGT
SmaI 8 CCCGGG CCC^GGG
SphI 1478 GCATGC GCATG^C
SspBI 920 TGTACA T^GTACA
SspI 1645 AATATT AAT^ATT
StuI 1806 AGGCCT AGG^CCT
StyI 1025 CCAAGG C^C[AT][AT]GG
Tsp509I 624 AATT ^AATT
XcmI 338 CCATGCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 8 CCCGGG C^CCGGG
XmaI 8 CCCGGG C^CCGGG
XmnI 614 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 35 enzymes) #
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AatII 16 GACGTC GACGT^C
497 GACGTC
AccI 376 GTCGAC GT^[AC][GT]AC
1606 GTCTAC
AhdI 352 GACGCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1957 GACTCTGAGTC
AspEI 352 GACGCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1957 GACTCTGAGTC
AvaI 8 CCCGGG C^[CT]CG[GA]G
1086 CTCGGG
AvaII 106 GGTCC G^G[AT]CC
763 GGTCC
BanI 933 GGTGCC G^G[CT][GA]CC
1795 GGTACC
BbvCI 1151 CCTCAGC CCTCAGC(-5/-2)
2017 CCTCAGC
BcnI 8 CCCGG CC^[GC]GG
9 CCGGG
BlpI 606 GCTCAGC GC^T[ACGT]AGC
1195 GCTCAGC
BmyI 890 GGGCCC G[AGT]GC[ACT]^C
1844 GTGCTC
BpmI 739 CTGGAG CTGGAG(16/14)
1340 CTGGAG
Bpu1102I 606 GCTCAGC GC^T[ACGT]AGC
1195 GCTCAGC
BsaI 1328 GAGACC GGTCTC(1/5)
1663 GAGACC
BsoBI 8 CCCGGG C^[CT]CG[GA]G
1086 CTCGGG
Bsp1286I 890 GGGCCC G[AGT]GC[ACT]^C
1844 GTGCTC
BspHI 1598 TCATGA T^CATGA
1689 TCATGA
BsrBI 706 GAGCGG CCGCTC(-3/-3)
757 GAGCGG
BstXI 197 CCATCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1025 CCAAGGAGATGG
BstYI 645 AGATCT [GA]^GATC[CT]
1059 AGATCC
CelII 606 GCTCAGC GC^T[ACGT]AGC
1195 GCTCAGC
DraIII 343 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
1907 CACGGAGTG
Eam1105I 352 GACGCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1957 GACTCTGAGTC
HincII 376 GTCGAC GT[CT]^[GA]AC
1465 GTCAAC
HindII 376 GTCGAC GT[CT]^[GA]AC
1465 GTCAAC
MflI 645 AGATCT [GA]^GATC[CT]
1059 AGATCC
MslI 198 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1281 CATGGATGTG
NciI 8 CCCGG CC^[GC]GG
9 CCGGG
PflMI 660 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1005 CCAGAGACTGG
RcaI 1598 TCATGA T^CATGA
1689 TCATGA
ScaI 866 AGTACT AGT^ACT
1921 AGTACT
SmlI 157 CTCAAG C^T[CT][GA]AG
529 CTCAAG
TfiI 1657 GATTC G^A[AT]TC
1943 GATTC
Van91I 660 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1005 CCAGAGACTGG
XhoII 645 AGATCT [GA]^GATC[CT]
1059 AGATCC
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI BbsI BbvI BfaI
BpuAI BsaHI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HgaI HhaI Hin1I HinP1I
HinfI HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MspA1I MvaI MvnI MwoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI Tru9I TseI
Tsp45I TspRI TthHB8I