Restriction map for
pF1KB9634
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AgeI Alw44I AlwI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI AspHI AspI AviII
AvrII BamHI BbeI BbrPI BbsI
BcgI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DpnII
DraI DraIII DrdI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI HphI
KasI Ksp632I KspI MaeII MamI
MboI MfeI MflI MluI MroI
MseI MunI NaeI NarI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 876 GGTACC G^GTACC
AccII 881 CGCG CG^CG
AflIII 847 ACATGT A^C[GA][CT]GT
AhdI 942 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 399 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 621 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 876 GGTACC G^GTACC
AspEI 942 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 110 TGGCCA TGG^CCA
BfaI 971 CTAG C^TAG
BsaI 949 GGTCTC GGTCTC(1/5)
BsmAI 950 GTCTC GTCTC(1/5)
BspLU11I 847 ACATGT A^CATGT
BsrFI 586 ACCGGC [GA]^CCGG[CT]
BstAPI 398 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 881 CGCG CG^CG
CfoI 882 GCGC GCG^C
Cfr10I 586 ACCGGC [GA]^CCGG[CT]
DsaI 101 CCATGG C^C[GA][CT]GG
Eam1105I 942 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HhaI 882 GCGC GCG^C
HinP1I 882 GCGC G^CGC
HinfI 39 GAGTC G^A[ACGT]TC
KpnI 876 GGTACC GGTAC^C
MaeI 971 CTAG C^TAG
MboII 869 TCTTC GAAGA(8/7)
MluNI 110 TGGCCA TGG^CCA
MscI 110 TGGCCA TGG^CCA
MvnI 881 CGCG CG^CG
NcoI 101 CCATGG C^CATGG
NspI 847 ACATGT [GA]CATG^[CT]
PflMI 645 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 39 GAGTC GAGTC(4/5)
SmlI 718 CTTGAG C^T[CT][GA]AG
StuI 504 AGGCCT AGG^CCT
TaqI 308 TCGA T^CGA
TthHB8I 308 TCGA T^CGA
Van91I 645 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 621 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 324 GTAC GT^AC
877 GTAC
ApaI 544 GGGCCC GGGCC^C
773 GGGCCC
AvaI 118 CCCGAG C^[CT]CG[GA]G
726 CTCGGG
BanI 699 GGCACC G^G[CT][GA]CC
876 GGTACC
BbvCI 236 CCTCAGC CCTCAGC(-5/-2)
272 CCTCAGC
BpmI 375 CTCCAG CTGGAG(16/14)
557 CTCCAG
BsoBI 118 CCCGAG C^[CT]CG[GA]G
726 CTCGGG
BsrBI 348 GAGCGG CCGCTC(-3/-3)
768 GAGCGG
BstXI 101 CCATGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
828 CCAGCATTATGG
EaeI 110 TGGCCA [CT]^GGCC[GA]
474 CGGCCA
EcoT14I 101 CCATGG C^C[AT][AT]GG
758 CCAAGG
MaeIII 53 GTGAC ^GT[ACGT]AC
748 GTGAC
MslI 162 CACCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
832 CATTATGGTG
PpuMI 45 GGGACCC [GA]G^G[AT]CC[CT]
940 GGGACCT
RsaI 324 GTAC GT^AC
877 GTAC
StyI 101 CCATGG C^C[AT][AT]GG
758 CCAAGG
Tsp45I 53 GTGAC ^GT[GC]AC
748 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BciVI BcnI BmyI BsaJI BseRI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DraII EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HpaII
ItaI MnlI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV PvuII
Sau96I ScrFI TseI TspRI XcmI