Restriction map for
pF1KB9641
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiWI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrGI BsrI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI DraI DraIII
DrdI Eam1105I EarI Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
Hin1I HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MroI MseI MunI NarI
NcoI NdeI NheI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AlwI 471 GGATC GGATC(4/5)
AlwNI 175 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 355 GAGCAC G[AT]GC[AT]^C
AviII 503 TGCGCA TGC^GCA
BalI 605 TGGCCA TGG^CCA
BsaI 913 GAGACC GGTCTC(1/5)
BsiHKAI 355 GAGCAC G[AT]GC[AT]^C
BsmBI 169 GAGACG CGTCTC(1/5)
BsrDI 561 CATTGC GCAATG(2/0)
BsrFI 642 GCCGGC [GA]^CCGG[CT]
BssSI 166 CACGAG CACGAG(-5/-1)
BstYI 471 GGATCT [GA]^GATC[CT]
Cfr10I 642 GCCGGC [GA]^CCGG[CT]
CpoI 5 CGGACCG CG^G[AT]CCG
DdeI 81 CTCAG C^T[ACGT]AG
DsaI 320 CCGTGG C^C[GA][CT]GG
EcoNI 393 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 242 CCAAGG C^C[AT][AT]GG
FspI 503 TGCGCA TGC^GCA
HincII 520 GTCAAC GT[CT]^[GA]AC
HindII 520 GTCAAC GT[CT]^[GA]AC
HinfI 840 GATTC G^A[ACGT]TC
MaeII 227 ACGT A^CGT
MaeIII 596 GTGAC ^GT[ACGT]AC
MflI 471 GGATCT [GA]^GATC[CT]
MluNI 605 TGGCCA TGG^CCA
MscI 605 TGGCCA TGG^CCA
NaeI 642 GCCGGC GCC^GGC
NgoMIV 642 GCCGGC G^CCGGC
NotI 491 GCGGCCGC GC^GGCCGC
PvuII 367 CAGCTG CAG^CTG
RsrII 5 CGGACCG CG^G[AT]CCG
SfaNI 506 GCATC GCATC(5/9)
SmlI 475 CTCAAG C^T[CT][GA]AG
StyI 242 CCAAGG C^C[AT][AT]GG
TfiI 840 GATTC G^A[AT]TC
Tsp45I 596 GTGAC ^GT[GC]AC
XhoII 471 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
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Aor51HI 212 AGCGCT AGC^GCT
686 AGCGCT
ApaI 146 GGGCCC GGGCC^C
372 GGGCCC
AvaI 75 CTCGGG C^[CT]CG[GA]G
655 CCCGAG
AvaII 6 GGACC G^G[AT]CC
390 GGACC
BanI 65 GGCACC G^G[CT][GA]CC
661 GGCACC
BsiEI 492 CGGCCG CG[GA][CT]^CG
644 CGGCCG
BsoBI 75 CTCGGG C^[CT]CG[GA]G
655 CCCGAG
BstF5I 201 CATCC GGATG(2/0)
507 CATCC
BstXI 188 CCAAGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
308 CCATGTCAGTGG
DpnII 261 GATC ^GATC
472 GATC
EagI 492 CGGCCG C^GGCCG
644 CGGCCG
EclXI 492 CGGCCG C^GGCCG
644 CGGCCG
Eco47III 212 AGCGCT AGC^GCT
686 AGCGCT
Eco52I 492 CGGCCG C^GGCCG
644 CGGCCG
FokI 201 CATCC GGATG(9/13)
507 CATCC
HgaI 171 GACGC GACGC(5/10)
598 GACGC
MboI 261 GATC ^GATC
472 GATC
MslI 309 CATGTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
768 CATCACCGTG
NdeII 261 GATC ^GATC
472 GATC
NlaIII 309 CATG CATG^
468 CATG
PstI 160 CTGCAG CTGCA^G
937 CTGCAG
Sau3AI 261 GATC ^GATC
472 GATC
ScaI 461 AGTACT AGT^ACT
818 AGTACT
SfcI 160 CTGCAG C^T[GA][CT]AG
937 CTGCAG
TspRI 126 CAGTG CA[GC]TG[ACGT][ACGT]^
314 CAGTG
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AccII AciI AfaI AluI BanII
BbvI BcnI BmyI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DraII EaeI EcoO109I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIV RsaI Sau96I
ScrFI TaqI TseI TthHB8I