Restriction map for
pF1KB9649
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AviII AvrII BalI
BamHI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BlnI
BpuAI BsaBI BsaI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI MaeI MaeIII
MamI MfeI MluNI MscI MseI
MslI MunI NcoI NdeI NheI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SbfI
ScaI SfaNI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp45I TspRI
Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccIII 213 TCCGGA T^CCGGA
AfaI 351 GTAC GT^AC
AflIII 161 ACGCGT A^C[GA][CT]GT
AlwI 285 GATCC GGATC(4/5)
Aor51HI 254 AGCGCT AGC^GCT
Asp700I 159 GAACGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 129 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BglII 233 AGATCT A^GATCT
BsaAI 592 TACGTG [CT]AC^GT[GA]
BsaWI 213 TCCGGA [AT]^CCGG[AT]
BseAI 213 TCCGGA T^CCGGA
BsmBI 801 GAGACG CGTCTC(1/5)
BspEI 213 TCCGGA T^CCGGA
BspMI 38 ACCTGC ACCTGC(4/8)
BssHII 1115 GCGCGC G^CGCGC
BstXI 239 CCAAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 512 AGGCCCC [GA]G^G[ACGT]CC[CT]
EarI 417 GAAGAG CTCTTC(1/4)
Eco47III 254 AGCGCT AGC^GCT
EcoO109I 512 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 73 GAATTC G^AATTC
HphI 953 TCACC GGTGA(8/7)
Ksp632I 417 GAAGAG CTCTTC(1/4)
MluI 161 ACGCGT A^CGCGT
MroI 213 TCCGGA T^CCGGA
NotI 520 GCGGCCGC GC^GGCCGC
NspI 218 ACATGC [GA]CATG^[CT]
PflFI 129 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 856 GAGTC GAGTC(4/5)
RsaI 351 GTAC GT^AC
SapI 417 GAAGAGC GCTCTTC(1/4)
SexAI 1307 ACCTGGT A^CC[AT]GGT
SfiI 513 GGCCCCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 44 CCCGGG CCC^GGG
TfiI 1198 GATTC G^A[AT]TC
Tth111I 129 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 44 CCCGGG C^CCGGG
XmaI 44 CCCGGG C^CCGGG
XmnI 159 GAACGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 73 GAATTC [GA]^AATT[CT]
1101 GAATTT
ApoI 73 GAATTC [GA]^AATT[CT]
1101 GAATTT
AvaII 176 GGTCC G^G[AT]CC
378 GGACC
BbeI 529 GGCGCC GGCGC^C
910 GGCGCC
BglI 514 GCCCCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
771 GCCGCCGGGGC
BlpI 840 GCTGAGC GC^T[ACGT]AGC
1260 GCTGAGC
BpmI 486 CTCCAG CTGGAG(16/14)
1288 CTGGAG
Bpu1102I 840 GCTGAGC GC^T[ACGT]AGC
1260 GCTGAGC
BsgI 1248 CTGCAC GTGCAG(16/14)
4 GTGCAG
BsmAI 801 GAGAC GTCTC(1/5)
210 GTCTC
BstAPI 491 GCAAGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
759 GCAGCTCCTGC
BstF5I 294 CATCC GGATG(2/0)
666 GGATG
BstYI 233 AGATCT [GA]^GATC[CT]
284 AGATCC
CelII 840 GCTGAGC GC^T[ACGT]AGC
1260 GCTGAGC
DsaI 518 CCGCGG C^C[GA][CT]GG
524 CCGCGG
EcoT14I 479 CCAAGG C^C[AT][AT]GG
533 CCAAGG
FokI 294 CATCC GGATG(9/13)
666 GGATG
HinfI 856 GAGTC G^A[ACGT]TC
1198 GATTC
KasI 529 GGCGCC G^GCGCC
910 GGCGCC
KspI 518 CCGCGG CCGC^GG
524 CCGCGG
MaeII 593 ACGT A^CGT
812 ACGT
MflI 233 AGATCT [GA]^GATC[CT]
284 AGATCC
NaeI 427 GCCGGC GCC^GGC
895 GCCGGC
NarI 529 GGCGCC GG^CGCC
910 GGCGCC
NgoMIV 427 GCCGGC G^CCGGC
895 GCCGGC
SacII 518 CCGCGG CCGC^GG
524 CCGCGG
SfoI 529 GGCGCC GGC^GCC
910 GGCGCC
SmlI 319 CTCAAG C^T[CT][GA]AG
508 CTCAAG
StyI 479 CCAAGG C^C[AT][AT]GG
533 CCAAGG
Tsp509I 74 AATT ^AATT
1102 AATT
XhoII 233 AGATCT [GA]^GATC[CT]
284 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AvaI BanI BanII BbvI BcnI
BmyI BsaHI BsaJI BseRI BsiEI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrBI BsrFI BsrI BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII EaeI EagI EclXI
Eco52I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII ItaI MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI PvuII Sau3AI Sau96I ScrFI
SfcI TaqI TseI TthHB8I