Restriction map for
pF1KB9672
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AocI
Aor51HI ApaI AscI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbvCI
BcgI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmFI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV FauI FseI FspI HaeII
HapII HgaI Hin1I HincII HindII
HpaI HpaII KasI KpnI KspI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XbaI XcmI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AciI 992 GCGG CCGC(-3/-1)
AflIII 204 ACATGT A^C[GA][CT]GT
Alw44I 354 GTGCAC G^TGCAC
AlwNI 871 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 354 GTGCAC G^TGCAC
AseI 115 ATTAAT AT^TAAT
AsnI 115 ATTAAT AT^TAAT
AvaI 920 CTCGAG C^[CT]CG[GA]G
AvaII 1110 GGACC G^G[AT]CC
BanI 899 GGCACC G^G[CT][GA]CC
BciVI 653 GTATCC GTATCC(6/5)
BclI 764 TGATCA T^GATCA
BfaI 1241 CTAG C^TAG
BsgI 230 GTGCAG GTGCAG(16/14)
BsiYI 327 CCTTCAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 327 CCTTCAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 78 GAGAC GTCTC(1/5)
BsmI 756 GCATTC GAATGC(1/-1)
BsoBI 920 CTCGAG C^[CT]CG[GA]G
BspLU11I 204 ACATGT A^CATGT
BsrDI 548 GCAATG GCAATG(2/0)
BstF5I 1032 CATCC GGATG(2/0)
CfoI 951 GCGC GCG^C
DraI 634 TTTAAA TTT^AAA
DsaI 1212 CCATGG C^C[GA][CT]GG
EaeI 1138 CGGCCA [CT]^GGCC[GA]
EcoT14I 1212 CCATGG C^C[AT][AT]GG
EcoT22I 964 ATGCAT ATGCA^T
FbaI 764 TGATCA T^GATCA
FokI 1032 CATCC GGATG(9/13)
HhaI 951 GCGC GCG^C
HinP1I 951 GCGC G^CGC
HindIII 362 AAGCTT A^AGCTT
MaeI 1241 CTAG C^TAG
MspA1I 680 CAGCTG C[AC]G^C[GT]G
MwoI 983 GCTTACAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 1212 CCATGG C^CATGG
NlaIV 899 GGCACC GG[ACGT]^[ACGT]CC
NsiI 964 ATGCAT ATGCA^T
PaeR7I 920 CTCGAG C^TCGAG
PshAI 421 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 115 ATTAAT AT^TAAT
PvuII 680 CAGCTG CAG^CTG
SmlI 920 CTCGAG C^T[CT][GA]AG
StuI 1243 AGGCCT AGG^CCT
StyI 1212 CCATGG C^C[AT][AT]GG
Tsp45I 603 GTCAC ^GT[GC]AC
XhoI 920 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AlwI 439 GATCC GGATC(4/5)
1134 GGATC
AspHI 272 GAGCAC G[AT]GC[AT]^C
354 GTGCAC
BbsI 1080 GAAGAC GAAGAC(2/6)
432 GTCTTC
BpmI 269 CTGGAG CTGGAG(16/14)
877 CTGGAG
BpuAI 1080 GAAGAC GAAGAC(2/6)
432 GTCTTC
BsiHKAI 272 GAGCAC G[AT]GC[AT]^C
354 GTGCAC
Cac8I 157 GCTTGC GC[ACGT]^[ACGT]GC
730 GCAAGC
Cfr13I 1055 GGCCC G^G[ACGT]CC
1110 GGACC
DraIII 139 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
340 CACATTGTG
EarI 720 CTCTTC CTCTTC(1/4)
538 GAAGAG
Ksp632I 720 CTCTTC CTCTTC(1/4)
538 GAAGAG
NspI 204 ACATGT [GA]CATG^[CT]
734 GCATGT
PleI 10 GAGTC GAGTC(4/5)
573 GAGTC
Sau96I 1055 GGCCC G^G[ACGT]CC
1110 GGACC
TaqI 689 TCGA T^CGA
921 TCGA
TthHB8I 689 TCGA T^CGA
921 TCGA
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
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AccI AcsI AfaI AluI ApoI
BbvI BmyI BsaJI Bsp1286I BsrI
BssKI BstNI DdeI DpnII EcoRII
Fnu4HI HaeIII HinfI HphI ItaI
MaeIII MboI MboII MnlI MseI
MslI MvaI NdeII NlaIII RsaI
Sau3AI ScrFI SfcI TfiI Tru9I
TseI Tsp509I TspRI