Restriction map for
pF1KB9673
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspHI AvaI AviII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvI BcgI BciVI BcnI
BfrI BglI BglII BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssKI BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstNI
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRII EcoRV FokI
FseI FspI HaeII HaeIII HapII
HgaI Hin1I HincII HindII HpaI
HpaII HphI KasI KpnI Ksp632I
MamI MfeI MflI MluI MluNI
MroI MscI MspI MunI MvaI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI RcaI RsrII SacI SalI
SapI SbfI ScrFI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TaqI TseI TthHB8I
Van91I XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 164 AAATTT [GA]^AATT[CT]
ApoI 164 AAATTT [GA]^AATT[CT]
AspI 504 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 337 CCTAGG C^CTAGG
BbvCI 617 CCTCAGC CCTCAGC(-5/-2)
BlnI 337 CCTAGG C^CTAGG
BpmI 587 CTCCAG CTGGAG(16/14)
BseRI 254 CTCCTC GAGGAG(10/8)
BsmAI 585 GTCTC GTCTC(1/5)
BsrI 225 CCAGT ACTGG(1/-1)
Cac8I 32 GCATGC GC[ACGT]^[ACGT]GC
CfoI 233 GCGC GCG^C
DraII 333 AGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 531 GACTCCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 436 CCGCGG C^C[GA][CT]GG
EcoO109I 333 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 337 CCTAGG C^C[AT][AT]GG
EcoT22I 725 ATGCAT ATGCA^T
Fnu4HI 230 GCCGC GC^[ACGT]GC
HhaI 233 GCGC GCG^C
HinP1I 233 GCGC G^CGC
HindIII 599 AAGCTT A^AGCTT
ItaI 230 GCCGC GC^[ACGT]GC
KspI 436 CCGCGG CCGC^GG
MaeII 741 ACGT A^CGT
MslI 681 CACTAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIV 658 GGGTCC GG[ACGT]^[ACGT]CC
NsiI 725 ATGCAT ATGCA^T
NspI 32 GCATGC [GA]CATG^[CT]
PflFI 504 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 333 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 590 CAGCTG CAG^CTG
SacII 436 CCGCGG CCGC^GG
ScaI 472 AGTACT AGT^ACT
SfcI 378 CTATAG C^T[GA][CT]AG
SphI 32 GCATGC GCATG^C
StyI 337 CCTAGG C^C[AT][AT]GG
TfiI 715 GAATC G^A[AT]TC
Tsp45I 139 GTGAC ^GT[GC]AC
TspRI 226 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 504 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 150 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 232 CGCG CG^CG
437 CGCG
AfaI 473 GTAC GT^AC
565 GTAC
AseI 640 ATTAAT AT^TAAT
651 ATTAAT
AsnI 640 ATTAAT AT^TAAT
651 ATTAAT
AvaII 334 GGTCC G^G[AT]CC
659 GGTCC
BclI 237 TGATCA T^GATCA
317 TGATCA
BsaJI 337 CCTAGG C^C[ACGT][ACGT]GG
436 CCGCGG
BsiYI 325 CCATATTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
478 CCCACACTAGG
BslI 325 CCATATTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
478 CCCACACTAGG
BstUI 232 CGCG CG^CG
437 CGCG
Cfr13I 334 GGTCC G^G[ACGT]CC
659 GGTCC
DpnII 238 GATC ^GATC
318 GATC
FauI 435 CCCGC CCCGC(4/6)
418 GCGGG
FbaI 237 TGATCA T^GATCA
317 TGATCA
MaeIII 139 GTGAC ^GT[ACGT]AC
457 GTTAC
MboI 238 GATC ^GATC
318 GATC
MboII 422 GAAGA GAAGA(8/7)
745 GAAGA
MspA1I 436 CCGCGG C[AC]G^C[GT]G
590 CAGCTG
MvnI 232 CGCG CG^CG
437 CGCG
NdeII 238 GATC ^GATC
318 GATC
PleI 531 GACTC GAGTC(4/5)
4 GAGTC
PshBI 640 ATTAAT AT^TAAT
651 ATTAAT
RsaI 473 GTAC GT^AC
565 GTAC
Sau3AI 238 GATC ^GATC
318 GATC
Sau96I 334 GGTCC G^G[ACGT]CC
659 GGTCC
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AciI AluI BfaI BsmFI DdeI
HinfI MaeI MnlI MseI MwoI
NlaIII SfaNI Tru9I Tsp509I