Restriction map for pF1KB9678
##################################
# NO CUT ENZYMES (  194 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbsI        BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BlnI        
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FseI        FspI        HapII       
HgaI        Hin1I       HindIII     HinfI       HpaI        
HpaII       KasI        KpnI        KspI        MaeI        
MaeII       MaeIII      MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MspA1I      MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StyI        SwaI        
TaqI        TfiI        Tru9I       Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   38 enzymes) #
###################################

AciI           33  GCGG                 CCGC(-3/-1)
BanII         226  GGGCTC               G[GA]GC[CT]^C
BbvCI          72  GCTGAGG              CCTCAGC(-5/-2)
BglII          11  AGATCT               A^GATCT
BlpI          111  GCTGAGC              GC^T[ACGT]AGC
BmyI          226  GGGCTC               G[AGT]GC[ACT]^C
Bpu1102I      111  GCTGAGC              GC^T[ACGT]AGC
BsaJI          26  CCAGGG               C^C[ACGT][ACGT]GG
Bsp1286I      226  GGGCTC               G[AGT]GC[ACT]^C
BspMI         248  ACCTGC               ACCTGC(4/8)
BsrBI          31  GAGCGG               CCGCTC(-3/-3)
BsrDI         139  GCAATG               GCAATG(2/0)
BstAPI        180  GCAAAGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I         84  CATCC                GGATG(2/0)
BstYI          11  AGATCT               [GA]^GATC[CT]
Cac8I         185  GCCTGC               GC[ACGT]^[ACGT]GC
CelII         111  GCTGAGC              GC^T[ACGT]AGC
CfoI           59  GCGC                 GCG^C
DpnII          12  GATC                 ^GATC
DraII         240  AGGACCC              [GA]G^G[ACGT]CC[CT]
EarI          229  CTCTTC               CTCTTC(1/4)
EcoO109I      240  AGGACCC              [GA]G^G[ACGT]CC[CT]
FokI           84  CATCC                GGATG(9/13)
HaeII          58  AGCGCC               [GA]GCGC^[CT]
HaeIII        256  GGCC                 GG^CC
HhaI           59  GCGC                 GCG^C
HinP1I         59  GCGC                 G^CGC
HincII         52  GTCAAC               GT[CT]^[GA]AC
HindII         52  GTCAAC               GT[CT]^[GA]AC
Ksp632I       229  CTCTTC               CTCTTC(1/4)
MboI           12  GATC                 ^GATC
MflI           11  AGATCT               [GA]^GATC[CT]
NdeII          12  GATC                 ^GATC
PpuMI         240  AGGACCC              [GA]G^G[AT]CC[CT]
SapI          228  GCTCTTC              GCTCTTC(1/4)
Sau3AI         12  GATC                 ^GATC
StuI          255  AGGCCT               AGG^CCT
XhoII          11  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

Asp700I         9  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              124  GAAACGCTTC          
AvaII         241  GGACC                G^G[AT]CC
              304  GGTCC               
BbvI           42  GCAGC                GCAGC(8/12)
              205  GCTGC               
BpmI           98  CTCCAG               CTGGAG(16/14)
              259  CTGGAG              
Cfr13I        241  GGACC                G^G[ACGT]CC
              304  GGTCC               
Fnu4HI         42  GCAGC                GC^[ACGT]GC
              205  GCTGC               
HphI           93  TCACC                GGTGA(8/7)
              298  TCACC               
ItaI           42  GCAGC                GC^[ACGT]GC
              205  GCTGC               
NlaIV         241  GGACCC               GG[ACGT]^[ACGT]CC
              291  GGTTCC              
Sau96I        241  GGACC                G^G[ACGT]CC
              304  GGTCC               
SexAI         287  ACCAGGT              A^CC[AT]GGT
              300  ACCTGGT             
SmlI          236  CTCAAG               C^T[CT][GA]AG
              277  CTTGAG              
TseI           42  GCAGC                G^C[AT]GC
              205  GCTGC               
XmnI            9  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              124  GAAACGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

AluI        BsiYI       BslI        BssKI       BstNI       
DdeI        EcoRII      MboII       MnlI        MvaI        
MwoI        ScrFI       
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