################################## # NO CUT ENZYMES ( 194 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HapII HgaI Hin1I HindIII HinfI HpaI HpaII KasI KpnI KspI MaeI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MseI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SbfI ScaI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AciI 33 GCGG CCGC(-3/-1) BanII 226 GGGCTC G[GA]GC[CT]^C BbvCI 72 GCTGAGG CCTCAGC(-5/-2) BglII 11 AGATCT A^GATCT BlpI 111 GCTGAGC GC^T[ACGT]AGC BmyI 226 GGGCTC G[AGT]GC[ACT]^C Bpu1102I 111 GCTGAGC GC^T[ACGT]AGC BsaJI 26 CCAGGG C^C[ACGT][ACGT]GG Bsp1286I 226 GGGCTC G[AGT]GC[ACT]^C BspMI 248 ACCTGC ACCTGC(4/8) BsrBI 31 GAGCGG CCGCTC(-3/-3) BsrDI 139 GCAATG GCAATG(2/0) BstAPI 180 GCAAAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 84 CATCC GGATG(2/0) BstYI 11 AGATCT [GA]^GATC[CT] Cac8I 185 GCCTGC GC[ACGT]^[ACGT]GC CelII 111 GCTGAGC GC^T[ACGT]AGC CfoI 59 GCGC GCG^C DpnII 12 GATC ^GATC DraII 240 AGGACCC [GA]G^G[ACGT]CC[CT] EarI 229 CTCTTC CTCTTC(1/4) EcoO109I 240 AGGACCC [GA]G^G[ACGT]CC[CT] FokI 84 CATCC GGATG(9/13) HaeII 58 AGCGCC [GA]GCGC^[CT] HaeIII 256 GGCC GG^CC HhaI 59 GCGC GCG^C HinP1I 59 GCGC G^CGC HincII 52 GTCAAC GT[CT]^[GA]AC HindII 52 GTCAAC GT[CT]^[GA]AC Ksp632I 229 CTCTTC CTCTTC(1/4) MboI 12 GATC ^GATC MflI 11 AGATCT [GA]^GATC[CT] NdeII 12 GATC ^GATC PpuMI 240 AGGACCC [GA]G^G[AT]CC[CT] SapI 228 GCTCTTC GCTCTTC(1/4) Sau3AI 12 GATC ^GATC StuI 255 AGGCCT AGG^CCT XhoII 11 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### Asp700I 9 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 124 GAAACGCTTC AvaII 241 GGACC G^G[AT]CC 304 GGTCC BbvI 42 GCAGC GCAGC(8/12) 205 GCTGC BpmI 98 CTCCAG CTGGAG(16/14) 259 CTGGAG Cfr13I 241 GGACC G^G[ACGT]CC 304 GGTCC Fnu4HI 42 GCAGC GC^[ACGT]GC 205 GCTGC HphI 93 TCACC GGTGA(8/7) 298 TCACC ItaI 42 GCAGC GC^[ACGT]GC 205 GCTGC NlaIV 241 GGACCC GG[ACGT]^[ACGT]CC 291 GGTTCC Sau96I 241 GGACC G^G[ACGT]CC 304 GGTCC SexAI 287 ACCAGGT A^CC[AT]GGT 300 ACCTGGT SmlI 236 CTCAAG C^T[CT][GA]AG 277 CTTGAG TseI 42 GCAGC G^C[AT]GC 205 GCTGC XmnI 9 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 124 GAAACGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### AluI BsiYI BslI BssKI BstNI DdeI EcoRII MboII MnlI MvaI MwoI ScrFI