##################################
# NO CUT ENZYMES (  241 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssKI       BssSI       Bst1107I    
BstBI       BstEII      BstNI       BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DdeI        DpnII       DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRII      EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        Fnu4HI      FseI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HinfI       
HpaI        HpaII       ItaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MspA1I      MspI        MunI        
MvaI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NlaIII      NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      Sau96I      SbfI        ScaI        ScrFI       
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TfiI        
TseI        Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (    9 enzymes) #
###################################

AluI           52  AGCT                 AG^CT
BstAPI        307  GCATATCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        224  CATCC                GGATG(2/0)
FokI          224  CATCC                GGATG(9/13)
HphI          172  GGTGA                GGTGA(8/7)
MboII         361  GAAGA                GAAGA(8/7)
MnlI          265  CCTC                 CCTC(7/6)
MslI          166  CACACTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI          307  GCATATCTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC


###################################
# TWO CUT ENZYMES (    2 enzymes) #
###################################

BpmI          254  CTGGAG               CTGGAG(16/14)
              338  CTGGAG              
Tsp45I        164  GTCAC                ^GT[GC]AC
              368  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (    6 enzymes) #
#########################################

BsrI        MaeIII      MseI        Tru9I       Tsp509I     
TspRI