Restriction map for
pF1KB9684
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MslI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AfaI 111 GTAC GT^AC
AlwI 371 GGATC GGATC(4/5)
Aor51HI 92 AGCGCT AGC^GCT
AspHI 274 GAGCAC G[AT]GC[AT]^C
AvaII 385 GGTCC G^G[AT]CC
BbsI 316 GAAGAC GAAGAC(2/6)
BbvCI 400 GCTGAGG CCTCAGC(-5/-2)
BmyI 274 GAGCAC G[AGT]GC[ACT]^C
BpuAI 316 GAAGAC GAAGAC(2/6)
BsaI 259 GAGACC GGTCTC(1/5)
BsiHKAI 274 GAGCAC G[AT]GC[AT]^C
BsiYI 263 CCTGGGTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 263 CCTGGGTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 412 GGGAC GGGAC(10/14)
Bsp1286I 274 GAGCAC G[AGT]GC[ACT]^C
BspHI 132 TCATGA T^CATGA
BspMI 351 GCAGGT ACCTGC(4/8)
BsrI 108 CCAGT ACTGG(1/-1)
BstF5I 32 CATCC GGATG(2/0)
BstYI 371 GGATCT [GA]^GATC[CT]
DpnII 372 GATC ^GATC
Eco47III 92 AGCGCT AGC^GCT
EcoNI 263 CCTGGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 32 CATCC GGATG(9/13)
HaeII 92 AGCGCT [GA]GCGC^[CT]
HphI 267 GGTGA GGTGA(8/7)
MaeIII 306 GTCAC ^GT[ACGT]AC
MboI 372 GATC ^GATC
MflI 371 GGATCT [GA]^GATC[CT]
MseI 143 TTAA T^TAA
NdeII 372 GATC ^GATC
NlaIII 133 CATG CATG^
PleI 467 GAGTC GAGTC(4/5)
RcaI 132 TCATGA T^CATGA
RsaI 111 GTAC GT^AC
Sau3AI 372 GATC ^GATC
TfiI 39 GAATC G^A[AT]TC
Tru9I 143 TTAA T^TAA
Tsp45I 306 GTCAC ^GT[GC]AC
Tsp509I 230 AATT ^AATT
XhoII 371 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BfaI 217 CTAG C^TAG
416 CTAG
BpmI 462 CTCCAG CTGGAG(16/14)
208 CTGGAG
BsmAI 259 GAGAC GTCTC(1/5)
431 GTCTC
CfoI 93 GCGC GCG^C
189 GCGC
DdeI 194 CTAAG C^T[ACGT]AG
401 CTGAG
EarI 472 CTCTTC CTCTTC(1/4)
484 CTCTTC
HaeIII 9 GGCC GG^CC
128 GGCC
HhaI 93 GCGC GCG^C
189 GCGC
HinP1I 93 GCGC G^CGC
189 GCGC
HinfI 39 GAATC G^A[ACGT]TC
467 GAGTC
Ksp632I 472 CTCTTC CTCTTC(1/4)
484 CTCTTC
MaeI 217 CTAG C^TAG
416 CTAG
MspA1I 122 CAGCTG C[AC]G^C[GT]G
407 CAGCTG
PvuII 122 CAGCTG CAG^CTG
407 CAGCTG
SfaNI 186 GATGC GCATC(5/9)
31 GCATC
SmlI 334 CTTGAG C^T[CT][GA]AG
452 CTCAAG
TspRI 277 CACTG CA[GC]TG[ACGT][ACGT]^
308 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AluI BbvI BsaJI BssKI BstNI
Cfr13I EcoRII Fnu4HI ItaI MboII
MnlI MvaI MwoI Sau96I ScrFI
TseI