Restriction map for
pF1KB9685
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# NO CUT ENZYMES ( 194 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspHI AspI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BbvI
BcgI BciVI BcnI BfaI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsiYI BslI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FauI Fnu4HI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII ItaI KasI KpnI
KspI MaeI MamI MfeI MflI
MluI MroI MslI MspA1I MspI
MunI MvnI MwoI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TseI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AfaI 373 GTAC GT^AC
AhdI 113 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 113 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 4 GGACC G^G[AT]CC
BalI 26 TGGCCA TGG^CCA
BbsI 121 GTCTTC GAAGAC(2/6)
BclI 155 TGATCA T^GATCA
BpuAI 121 GTCTTC GAAGAC(2/6)
BsaJI 126 CCTTGG C^C[ACGT][ACGT]GG
BseRI 46 GAGGAG GAGGAG(10/8)
BsgI 68 CTGCAC GTGCAG(16/14)
BsmFI 3 GGGAC GGGAC(10/14)
BsrDI 55 CATTGC GCAATG(2/0)
Cfr13I 4 GGACC G^G[ACGT]CC
DdeI 143 CTAAG C^T[ACGT]AG
DpnII 156 GATC ^GATC
EaeI 26 TGGCCA [CT]^GGCC[GA]
Eam1105I 113 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 195 GAAGAG CTCTTC(1/4)
EcoRI 34 GAATTC G^AATTC
EcoT14I 126 CCTTGG C^C[AT][AT]GG
FbaI 155 TGATCA T^GATCA
HaeIII 27 GGCC GG^CC
HindIII 539 AAGCTT A^AGCTT
HinfI 277 GATTC G^A[ACGT]TC
HphI 158 TCACC GGTGA(8/7)
Ksp632I 195 GAAGAG CTCTTC(1/4)
MaeII 315 ACGT A^CGT
MboI 156 GATC ^GATC
MluNI 26 TGGCCA TGG^CCA
MscI 26 TGGCCA TGG^CCA
NdeI 446 CATATG CA^TATG
NdeII 156 GATC ^GATC
NlaIII 202 CATG CATG^
NlaIV 3 GGGACC GG[ACGT]^[ACGT]CC
PshAI 250 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 373 GTAC GT^AC
Sau3AI 156 GATC ^GATC
Sau96I 4 GGACC G^G[ACGT]CC
SexAI 116 ACCTGGT A^CC[AT]GGT
SfcI 108 CTACAG C^T[GA][CT]AG
StyI 126 CCTTGG C^C[AT][AT]GG
TfiI 277 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AluI 414 AGCT AG^CT
540 AGCT
BpmI 43 CTGGAG CTGGAG(16/14)
166 CTGGAG
BsmAI 198 GAGAC GTCTC(1/5)
457 GTCTC
BssKI 60 CCTGG ^CC[ACGT]GG
117 CCTGG
BstF5I 90 GGATG GGATG(2/0)
360 GGATG
BstNI 60 CCTGG CC^[AT]GG
117 CCTGG
EcoRII 60 CCTGG ^CC[AT]GG
117 CCTGG
FokI 90 GGATG GGATG(9/13)
360 GGATG
MaeIII 139 GTCAC ^GT[ACGT]AC
298 GTGAC
MboII 195 GAAGA GAAGA(8/7)
122 TCTTC
MseI 337 TTAA T^TAA
398 TTAA
MvaI 60 CCTGG CC^[AT]GG
117 CCTGG
ScrFI 60 CCTGG CC^[ACGT]GG
117 CCTGG
Tru9I 337 TTAA T^TAA
398 TTAA
Tsp45I 139 GTCAC ^GT[GC]AC
298 GTGAC
TspRI 66 CACTG CA[GC]TG[ACGT][ACGT]^
189 CACTG
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# 3 OR MORE CUT ENZYMES ( 5 enzymes) #
#########################################
AcsI ApoI BsrI MnlI Tsp509I