Restriction map for
pF1KB9690
##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AflIII AhdI Alw44I AlwI AlwNI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BamHI BbrPI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BsaAI BsaBI BsaI BsiHKAI BsiWI
BsmAI BsmBI BsmI BspHI BspLU11I
BspMI BsrDI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
CpoI DraI DraII DrdI Eam1105I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HinfI HpaI KpnI
MaeI MaeIII MamI MfeI MflI
MluI MseI MslI MunI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfuI SmlI SnaBI SpeI SphI
Sse8387I SspI SwaI TfiI Tru9I
Tsp45I Tth111I XbaI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AatII 73 GACGTC GACGT^C
AccIII 503 TCCGGA T^CCGGA
AgeI 295 ACCGGT A^CCGGT
AocI 274 CCTCAGG CC^T[ACGT]AGG
ApaI 106 GGGCCC GGGCC^C
Asp700I 552 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 608 TGGCCA TGG^CCA
BbeI 84 GGCGCC GGCGC^C
BbsI 169 GAAGAC GAAGAC(2/6)
BcgI 304 CGAGAGCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 348 GCTGAGC GC^T[ACGT]AGC
BpmI 58 CTGGAG CTGGAG(16/14)
Bpu1102I 348 GCTGAGC GC^T[ACGT]AGC
BpuAI 169 GAAGAC GAAGAC(2/6)
BseAI 503 TCCGGA T^CCGGA
BseRI 196 GAGGAG GAGGAG(10/8)
BsgI 312 CTGCAC GTGCAG(16/14)
BsiEI 26 CGGCCG CG[GA][CT]^CG
Bsp1407I 659 TGTACA T^GTACA
BspDI 43 ATCGAT AT^CGAT
BspEI 503 TCCGGA T^CCGGA
BsrGI 659 TGTACA T^GTACA
BstF5I 218 GGATG GGATG(2/0)
BstXI 599 CCAGTTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 274 CCTCAGG CC^T[ACGT]AGG
CelII 348 GCTGAGC GC^T[ACGT]AGC
ClaI 43 ATCGAT AT^CGAT
DpnII 42 GATC ^GATC
DraIII 385 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 26 CGGCCG C^GGCCG
EclXI 26 CGGCCG C^GGCCG
Eco52I 26 CGGCCG C^GGCCG
Eco81I 274 CCTCAGG CC^T[ACGT]AGG
EcoT14I 633 CCTTGG C^C[AT][AT]GG
FokI 218 GGATG GGATG(9/13)
HphI 283 TCACC GGTGA(8/7)
KasI 84 GGCGCC G^GCGCC
MaeII 74 ACGT A^CGT
MboI 42 GATC ^GATC
MluNI 608 TGGCCA TGG^CCA
MroI 503 TCCGGA T^CCGGA
MscI 608 TGGCCA TGG^CCA
NarI 84 GGCGCC GG^CGCC
NdeII 42 GATC ^GATC
NlaIII 483 CATG CATG^
PflMI 384 CCACGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 295 ACCGGT A^CCGGT
Sau3AI 42 GATC ^GATC
SfaNI 219 GATGC GCATC(5/9)
SfcI 645 CTACAG C^T[GA][CT]AG
SfoI 84 GGCGCC GGC^GCC
SgrAI 119 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 659 TGTACA T^GTACA
StuI 278 AGGCCT AGG^CCT
StyI 633 CCTTGG C^C[AT][AT]GG
Tsp509I 556 AATT ^AATT
TspRI 442 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 384 CCACGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 449 CCAACTGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 552 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 73 GACGTC G[GA]^CG[CT]C
84 GGCGCC
AfaI 576 GTAC GT^AC
660 GTAC
Aor51HI 308 AGCGCT AGC^GCT
416 AGCGCT
BanI 84 GGCGCC G^G[CT][GA]CC
292 GGCACC
BanII 106 GGGCCC G[GA]GC[CT]^C
593 GAGCCC
BmyI 106 GGGCCC G[AGT]GC[ACT]^C
593 GAGCCC
BsaHI 73 GACGTC G[GA]^CG[CT]C
84 GGCGCC
BsaWI 295 ACCGGT [AT]^CCGG[AT]
503 TCCGGA
Bsp1286I 106 GGGCCC G[AGT]GC[ACT]^C
593 GAGCCC
BsrBI 265 CCGCTC CCGCTC(-3/-3)
469 GAGCGG
BssHII 368 GCGCGC G^CGCGC
370 GCGCGC
BstNI 57 CCTGG CC^[AT]GG
492 CCAGG
DdeI 275 CTCAG C^T[ACGT]AG
349 CTGAG
EarI 67 GAAGAG CTCTTC(1/4)
181 GAAGAG
Eco47III 308 AGCGCT AGC^GCT
416 AGCGCT
EcoRII 57 CCTGG ^CC[AT]GG
492 CCAGG
Hin1I 73 GACGTC G[GA]^CG[CT]C
84 GGCGCC
Ksp632I 67 GAAGAG CTCTTC(1/4)
181 GAAGAG
MvaI 57 CCTGG CC^[AT]GG
492 CCAGG
NaeI 120 GCCGGC GCC^GGC
289 GCCGGC
NgoMIV 120 GCCGGC G^CCGGC
289 GCCGGC
RsaI 576 GTAC GT^AC
660 GTAC
SmaI 103 CCCGGG CCC^GGG
214 CCCGGG
XmaCI 103 CCCGGG C^CCGGG
214 CCCGGG
XmaI 103 CCCGGG C^CCGGG
214 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccII AciI AluI AvaI AvaII
BbvI BcnI BglI BsaJI BsiYI
BslI BsmFI BsoBI BsrFI BsrI
BssKI BstUI Cac8I CfoI Cfr10I
Cfr13I DsaI EaeI FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
HinP1I HpaII ItaI KspI MboII
MnlI MspA1I MspI MvnI MwoI
NciI NlaIV SacII Sau96I ScrFI
TaqI TseI TthHB8I