Restriction map for
pF1KB9691
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BbrPI
BciVI BclI BfaI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaI
BsaWI BseAI BsgI BsiHKAI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BsrI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HindIII HinfI
HpaI KpnI MaeI MaeII MfeI
MluI MluNI MroI MscI MslI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfuI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tth111I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Asp700I 438 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 337 GCTGAGG CCTCAGC(-5/-2)
BglII 524 AGATCT A^GATCT
BsaBI 519 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 146 CGACCG CG[GA][CT]^CG
BsmFI 49 GGGAC GGGAC(10/14)
BspMI 236 ACCTGC ACCTGC(4/8)
BsrFI 130 GCCGGC [GA]^CCGG[CT]
BssHII 320 GCGCGC G^CGCGC
BstYI 524 AGATCT [GA]^GATC[CT]
Cfr10I 130 GCCGGC [GA]^CCGG[CT]
DdeI 338 CTGAG C^T[ACGT]AG
DpnII 525 GATC ^GATC
DraII 378 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 635 CCGCGG C^C[GA][CT]GG
EarI 670 GAAGAG CTCTTC(1/4)
EcoO109I 378 AGGACCT [GA]G^G[ACGT]CC[CT]
HincII 169 GTCAAC GT[CT]^[GA]AC
HindII 169 GTCAAC GT[CT]^[GA]AC
HphI 506 TCACC GGTGA(8/7)
Ksp632I 670 GAAGAG CTCTTC(1/4)
KspI 635 CCGCGG CCGC^GG
MaeIII 281 GTGAC ^GT[ACGT]AC
MamI 519 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 525 GATC ^GATC
MflI 524 AGATCT [GA]^GATC[CT]
MseI 521 TTAA T^TAA
MspA1I 635 CCGCGG C[AC]G^C[GT]G
NaeI 130 GCCGGC GCC^GGC
NdeII 525 GATC ^GATC
NgoMIV 130 GCCGGC G^CCGGC
PflMI 420 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 378 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 571 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 276 CTGCAG CTGCA^G
SacII 635 CCGCGG CCGC^GG
Sau3AI 525 GATC ^GATC
SfcI 276 CTGCAG C^T[GA][CT]AG
SgrAI 129 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 268 CCCGGG CCC^GGG
TaqI 145 TCGA T^CGA
Tru9I 521 TTAA T^TAA
Tsp45I 281 GTGAC ^GT[GC]AC
Tsp509I 346 AATT ^AATT
TthHB8I 145 TCGA T^CGA
Van91I 420 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 524 AGATCT [GA]^GATC[CT]
XmaCI 268 CCCGGG C^CCGGG
XmaI 268 CCCGGG C^CCGGG
XmnI 438 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AluI 431 AGCT AG^CT
594 AGCT
AlwNI 377 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
421 CAGACGCTG
AvaI 268 CCCGGG C^[CT]CG[GA]G
598 CCCGAG
AvaII 379 GGACC G^G[AT]CC
577 GGTCC
BanII 189 GAGCCC G[GA]GC[CT]^C
271 GGGCTC
BbsI 676 GAAGAC GAAGAC(2/6)
372 GTCTTC
BcgI 278 GCAGTGACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
479 GCATTGAAATCG
BpmI 427 CTGGAG CTGGAG(16/14)
433 CTGGAG
BpuAI 676 GAAGAC GAAGAC(2/6)
372 GTCTTC
BseRI 652 GAGGAG GAGGAG(10/8)
682 GAGGAG
BsiYI 362 CCTGGATGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
420 CCAGACGCTGG
BslI 362 CCTGGATGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
420 CCAGACGCTGG
BsoBI 268 CCCGGG C^[CT]CG[GA]G
598 CCCGAG
BstF5I 264 CATCC GGATG(2/0)
365 GGATG
BstNI 213 CCTGG CC^[AT]GG
362 CCTGG
EcoRII 213 CCTGG ^CC[AT]GG
362 CCTGG
FokI 264 CATCC GGATG(9/13)
365 GGATG
MvaI 213 CCTGG CC^[AT]GG
362 CCTGG
NlaIII 328 CATG CATG^
546 CATG
SfaNI 366 GATGC GCATC(5/9)
353 GCATC
TspRI 279 CAGTG CA[GC]TG[ACGT][ACGT]^
627 CACTG
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AccII AciI AcyI BanI BbeI
BbvI BcnI BmyI BsaHI BsaJI
Bsp1286I BssKI BstUI Cac8I CfoI
Cfr13I FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII ItaI KasI MboII MnlI
MspI MvnI MwoI NarI NciI
NlaIV Sau96I ScrFI SfoI TseI