Restriction map for
pF1KB9692
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvrII BalI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BseAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DdeI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
HaeII Hin1I HpaI HphI KasI
KpnI KspI MaeI MaeII MaeIII
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tsp45I Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 708 CGCG CG^CG
ApaI 680 GGGCCC GGGCC^C
Asp700I 486 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 3 GAGCTC G[AT]GC[AT]^C
AviII 294 TGCGCA TGC^GCA
BamHI 300 GGATCC G^GATCC
BglI 682 GCCCCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 587 ACCGGA [AT]^CCGG[AT]
BsgI 313 GTGCAG GTGCAG(16/14)
BsiEI 99 CGGCCG CG[GA][CT]^CG
BsiHKAI 3 GAGCTC G[AT]GC[AT]^C
BsmAI 561 GAGAC GTCTC(1/5)
BsrBI 676 GAGCGG CCGCTC(-3/-3)
BstAPI 635 GCAGCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 708 CGCG CG^CG
BstXI 344 CCAGCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 300 GGATCC [GA]^GATC[CT]
DpnII 301 GATC ^GATC
EaeI 99 CGGCCG [CT]^GGCC[GA]
EagI 99 CGGCCG C^GGCCG
EarI 400 CTCTTC CTCTTC(1/4)
EclXI 99 CGGCCG C^GGCCG
Eco52I 99 CGGCCG C^GGCCG
EcoNI 351 CCTGGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 294 TGCGCA TGC^GCA
HincII 16 GTCAAC GT[CT]^[GA]AC
HindII 16 GTCAAC GT[CT]^[GA]AC
HindIII 331 AAGCTT A^AGCTT
Ksp632I 400 CTCTTC CTCTTC(1/4)
MboI 301 GATC ^GATC
MboII 401 TCTTC GAAGA(8/7)
MflI 300 GGATCC [GA]^GATC[CT]
MseI 500 TTAA T^TAA
MslI 108 CACCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 708 CGCG CG^CG
NdeII 301 GATC ^GATC
SacI 3 GAGCTC GAGCT^C
SapI 399 GCTCTTC GCTCTTC(1/4)
Sau3AI 301 GATC ^GATC
SexAI 308 ACCTGGT A^CC[AT]GGT
SfaNI 411 GATGC GCATC(5/9)
SfcI 456 CTACAG C^T[GA][CT]AG
TaqI 530 TCGA T^CGA
TfiI 527 GAATC G^A[AT]TC
Tru9I 500 TTAA T^TAA
TspRI 23 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 530 TCGA T^CGA
XhoII 300 GGATCC [GA]^GATC[CT]
XmnI 486 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AfaI 117 GTAC GT^AC
318 GTAC
AlwI 301 GATCC GGATC(4/5)
300 GGATC
BcnI 44 CCGGG CC^[GC]GG
244 CCCGG
BseRI 358 GAGGAG GAGGAG(10/8)
652 GAGGAG
BstF5I 410 GGATG GGATG(2/0)
593 GGATG
FokI 410 GGATG GGATG(9/13)
593 GGATG
HgaI 434 GACGC GACGC(5/10)
706 GACGC
NciI 44 CCGGG CC^[GC]GG
244 CCCGG
PflMI 87 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
468 CCAGACCCTGG
PleI 513 GACTC GAGTC(4/5)
49 GAGTC
PpuMI 89 AGGACCT [GA]G^G[AT]CC[CT]
239 GGGACCC
RsaI 117 GTAC GT^AC
318 GTAC
Tsp509I 64 AATT ^AATT
250 AATT
Van91I 87 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
468 CCAGACCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI AlwNI AvaII BanII
BbvI BmyI BpmI BsaJI BsiYI
BslI BsmFI Bsp1286I BssKI BstNI
Cac8I CfoI Cfr13I DraII DsaI
EcoO109I EcoRII FauI Fnu4HI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MnlI MspA1I MspI MvaI
MwoI NlaIV Sau96I ScrFI TseI