Restriction map for
pF1KB9702
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AocI
ApaI ApoI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BalI BamHI BbrPI BbsI BbvCI
BciVI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII Eam1105I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
MaeII MamI MfeI MluI MluNI
MroI MscI MseI MslI MunI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I TspRI Tth111I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 674 GGTACC G^GTACC
Alw44I 332 GTGCAC G^TGCAC
AlwI 23 GGATC GGATC(4/5)
Aor51HI 753 AGCGCT AGC^GCT
ApaLI 332 GTGCAC G^TGCAC
Asp718I 674 GGTACC G^GTACC
BanII 628 GAGCTC G[GA]GC[CT]^C
BbeI 913 GGCGCC GGCGC^C
BclI 290 TGATCA T^GATCA
BfaI 344 CTAG C^TAG
BglII 488 AGATCT A^GATCT
BsaI 873 GAGACC GGTCTC(1/5)
BsgI 613 GTGCAG GTGCAG(16/14)
BsmBI 178 GAGACG CGTCTC(1/5)
BsrBI 592 GAGCGG CCGCTC(-3/-3)
BsrI 261 CCAGT ACTGG(1/-1)
BstAPI 134 GCAGCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 488 AGATCT [GA]^GATC[CT]
DrdI 655 GACACGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 710 CGGCCG C^GGCCG
EarI 144 CTCTTC CTCTTC(1/4)
EclXI 710 CGGCCG C^GGCCG
Eco47III 753 AGCGCT AGC^GCT
Eco52I 710 CGGCCG C^GGCCG
EcoNI 63 CCTTCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 290 TGATCA T^GATCA
HgaI 953 GCGTC GACGC(5/10)
KasI 913 GGCGCC G^GCGCC
KpnI 674 GGTACC GGTAC^C
Ksp632I 144 CTCTTC CTCTTC(1/4)
MaeI 344 CTAG C^TAG
MaeIII 274 GTCAC ^GT[ACGT]AC
MboII 145 TCTTC GAAGA(8/7)
MflI 488 AGATCT [GA]^GATC[CT]
NarI 913 GGCGCC GG^CGCC
NlaIII 624 CATG CATG^
PshAI 708 GACGGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 232 CTGCAG CTGCA^G
SacI 628 GAGCTC GAGCT^C
SapI 143 GCTCTTC GCTCTTC(1/4)
SexAI 670 ACCTGGT A^CC[AT]GGT
SfcI 232 CTGCAG C^T[GA][CT]AG
SfoI 913 GGCGCC GGC^GCC
SmaI 238 CCCGGG CCC^GGG
Tsp45I 274 GTCAC ^GT[GC]AC
Tsp509I 745 AATT ^AATT
XhoII 488 AGATCT [GA]^GATC[CT]
XmaCI 238 CCCGGG C^CCGGG
XmaI 238 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 913 GGCGCC G[GA]^CG[CT]C
952 GGCGTC
AspHI 332 GTGCAC G[AT]GC[AT]^C
628 GAGCTC
AvaI 85 CTCGGG C^[CT]CG[GA]G
238 CCCGGG
BcgI 314 GCAAACCACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
623 GCATGGAGCTCG
BsaHI 913 GGCGCC G[GA]^CG[CT]C
952 GGCGTC
BseRI 279 CTCCTC GAGGAG(10/8)
472 GAGGAG
BsiEI 480 CGACCG CG[GA][CT]^CG
710 CGGCCG
BsiHKAI 332 GTGCAC G[AT]GC[AT]^C
628 GAGCTC
BsoBI 85 CTCGGG C^[CT]CG[GA]G
238 CCCGGG
BspMI 230 ACCTGC ACCTGC(4/8)
660 GCAGGT
BsrFI 357 GCCGGC [GA]^CCGG[CT]
949 GCCGGC
BssHII 541 GCGCGC G^CGCGC
851 GCGCGC
Cfr10I 357 GCCGGC [GA]^CCGG[CT]
949 GCCGGC
DraII 74 GGGACCC [GA]G^G[ACGT]CC[CT]
184 GGGACCC
EcoO109I 74 GGGACCC [GA]G^G[ACGT]CC[CT]
184 GGGACCC
EcoT14I 68 CCAAGG C^C[AT][AT]GG
392 CCAAGG
Hin1I 913 GGCGCC G[GA]^CG[CT]C
952 GGCGTC
KspI 398 CCGCGG CCGC^GG
840 CCGCGG
NaeI 357 GCCGGC GCC^GGC
949 GCCGGC
NgoMIV 357 GCCGGC G^CCGGC
949 GCCGGC
PflMI 190 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
561 CCATCAGCTGG
PpuMI 74 GGGACCC [GA]G^G[AT]CC[CT]
184 GGGACCC
PvuII 565 CAGCTG CAG^CTG
574 CAGCTG
SacII 398 CCGCGG CCGC^GG
840 CCGCGG
SfaNI 205 GCATC GCATC(5/9)
890 GCATC
StyI 68 CCAAGG C^C[AT][AT]GG
392 CCAAGG
TaqI 26 TCGA T^CGA
590 TCGA
TthHB8I 26 TCGA T^CGA
590 TCGA
Van91I 190 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
561 CCATCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AvaII BanI BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MboI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI TseI