Restriction map for
pF1KB9717
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI BsmFI BsoBI Bsp1407I BspDI
BspEI BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I Cfr13I ClaI CpoI DraII
DraIII EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HaeIII
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII HphI KasI
KpnI Ksp632I KspI MfeI MflI
MluI MluNI MroI MscI MspA1I
MspI MunI NaeI NarI NciI
NdeI NgoMIV NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI Sau96I SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccII 616 CGCG CG^CG
AcsI 998 AAATTC [GA]^AATT[CT]
ApoI 998 AAATTC [GA]^AATT[CT]
Asp700I 103 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 142 GAGCAC G[AT]GC[AT]^C
BbvCI 763 CCTCAGC CCTCAGC(-5/-2)
BclI 288 TGATCA T^GATCA
BmyI 142 GAGCAC G[AGT]GC[ACT]^C
BsaAI 651 CACGTA [CT]AC^GT[GA]
BsaBI 289 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 713 CTCCTC GAGGAG(10/8)
BsiHKAI 142 GAGCAC G[AT]GC[AT]^C
BsiYI 180 CCTTTATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 180 CCTTTATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 736 GAATGC GAATGC(1/-1)
Bsp1286I 142 GAGCAC G[AGT]GC[ACT]^C
BspHI 1125 TCATGA T^CATGA
BspLU11I 1065 ACATGT A^CATGT
BspMI 412 ACCTGC ACCTGC(4/8)
BsrDI 774 CATTGC GCAATG(2/0)
BsrI 643 ACTGG ACTGG(1/-1)
BssSI 635 CTCGTG CACGAG(-5/-1)
BstUI 616 CGCG CG^CG
Cac8I 721 GCTAGC GC[ACGT]^[ACGT]GC
DrdI 482 GACATACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 288 TGATCA T^GATCA
MaeIII 322 GTAAC ^GT[ACGT]AC
MamI 289 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 616 CGCG CG^CG
MwoI 863 GCTATTTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NheI 721 GCTAGC G^CTAGC
PleI 462 GACTC GAGTC(4/5)
PstI 605 CTGCAG CTGCA^G
RcaI 1125 TCATGA T^CATGA
SfcI 605 CTGCAG C^T[GA][CT]AG
SmlI 726 CTCAAG C^T[CT][GA]AG
XmnI 103 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 244 ACGTGT A^C[GA][CT]GT
1065 ACATGT
BbsI 103 GAAGAC GAAGAC(2/6)
214 GAAGAC
BbvI 515 GCAGC GCAGC(8/12)
571 GCAGC
BfaI 722 CTAG C^TAG
1006 CTAG
BpuAI 103 GAAGAC GAAGAC(2/6)
214 GAAGAC
CfoI 174 GCGC GCG^C
820 GCGC
DraI 225 TTTAAA TTT^AAA
252 TTTAAA
DsaI 147 CCATGG C^C[GA][CT]GG
417 CCATGG
EcoT14I 147 CCATGG C^C[AT][AT]GG
417 CCATGG
Fnu4HI 515 GCAGC GC^[ACGT]GC
571 GCAGC
HhaI 174 GCGC GCG^C
820 GCGC
HinP1I 174 GCGC G^CGC
820 GCGC
ItaI 515 GCAGC GC^[ACGT]GC
571 GCAGC
MaeI 722 CTAG C^TAG
1006 CTAG
NcoI 147 CCATGG C^CATGG
417 CCATGG
NspI 473 ACATGC [GA]CATG^[CT]
1065 ACATGT
ScaI 498 AGTACT AGT^ACT
890 AGTACT
SspI 281 AATATT AAT^ATT
541 AATATT
StyI 147 CCATGG C^C[AT][AT]GG
417 CCATGG
TaqI 159 TCGA T^CGA
492 TCGA
TseI 515 GCAGC G^C[AT]GC
571 GCAGC
TthHB8I 159 TCGA T^CGA
492 TCGA
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AfaI AluI AlwI BsaJI BssKI
BstF5I BstNI DdeI DpnII EcoRII
FokI HinfI MaeII MboI MboII
MnlI MseI MslI MvaI NdeII
NlaIII RsaI Sau3AI ScrFI SfaNI
TfiI Tru9I Tsp509I TspRI