Restriction map for
pF1KB9720
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwI
AocI ApaI ApaLI ApoI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BcgI BciVI
BfaI BfrI BglII BlnI BsaAI
BsaBI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstBI BstXI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DraII DraIII DrdI Eam1105I
Eco81I EcoNI EcoO109I EcoRI EcoRV
EcoT22I FseI FspI HindIII HpaI
KpnI KspI MaeI MamI MfeI
MflI MluI MroI MseI MunI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SfiI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 889 GTCGAC GT^[AC][GT]AC
AflIII 602 ACGTGT A^C[GA][CT]GT
AlwNI 514 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1043 AGCGCT AGC^GCT
AscI 648 GGCGCGCC GG^CGCGCC
Asp700I 342 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 721 GAGCTC G[AT]GC[AT]^C
BalI 2 TGGCCA TGG^CCA
BbvCI 261 CCTCAGC CCTCAGC(-5/-2)
BclI 1154 TGATCA T^GATCA
BlpI 526 GCTGAGC GC^T[ACGT]AGC
BpmI 714 CTGGAG CTGGAG(16/14)
Bpu1102I 526 GCTGAGC GC^T[ACGT]AGC
BsaI 473 GAGACC GGTCTC(1/5)
BsaWI 311 ACCGGA [AT]^CCGG[AT]
BsiHKAI 721 GAGCTC G[AT]GC[AT]^C
BsmBI 334 CGTCTC CGTCTC(1/5)
BstEII 9 GGTGACC G^GT[ACGT]ACC
BstPI 9 GGTGACC G^GT[ACGT]ACC
CelII 526 GCTGAGC GC^T[ACGT]AGC
Eco47III 1043 AGCGCT AGC^GCT
EcoO65I 9 GGTGACC G^GT[ACGT]ACC
EcoT14I 5 CCATGG C^C[AT][AT]GG
FbaI 1154 TGATCA T^GATCA
HincII 889 GTCGAC GT[CT]^[GA]AC
HindII 889 GTCGAC GT[CT]^[GA]AC
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MslI 199 CATTACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 787 GCCGGC GCC^GGC
NcoI 5 CCATGG C^CATGG
NgoMIV 787 GCCGGC G^CCGGC
NotI 383 GCGGCCGC GC^GGCCGC
NspI 1163 ACATGC [GA]CATG^[CT]
SacI 721 GAGCTC GAGCT^C
SalI 889 GTCGAC G^TCGAC
ScaI 326 AGTACT AGT^ACT
StyI 5 CCATGG C^C[AT][AT]GG
XmnI 342 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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BbeI 768 GGCGCC GGCGC^C
1052 GGCGCC
BbsI 1134 GAAGAC GAAGAC(2/6)
208 GTCTTC
BglI 29 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
434 GCCCCCCGGGC
BpuAI 1134 GAAGAC GAAGAC(2/6)
208 GTCTTC
BseRI 423 CTCCTC GAGGAG(10/8)
139 GAGGAG
BsgI 760 CTGCAC GTGCAG(16/14)
376 GTGCAG
BstAPI 396 GCACTCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
513 GCAGCTGCTGC
BstF5I 249 CATCC GGATG(2/0)
390 CATCC
FokI 249 CATCC GGATG(9/13)
390 CATCC
HinfI 91 GACTC G^A[ACGT]TC
892 GACTC
KasI 768 GGCGCC G^GCGCC
1052 GGCGCC
MaeII 602 ACGT A^CGT
968 ACGT
MaeIII 10 GTGAC ^GT[ACGT]AC
287 GTGAC
MspA1I 514 CAGCTG C[AC]G^C[GT]G
1108 CAGCTG
NarI 768 GGCGCC GG^CGCC
1052 GGCGCC
PleI 91 GACTC GAGTC(4/5)
892 GACTC
PvuII 514 CAGCTG CAG^CTG
1108 CAGCTG
SbfI 882 CCTGCAGG CCTGCA^GG
978 CCTGCAGG
SexAI 689 ACCAGGT A^CC[AT]GGT
839 ACCAGGT
SfoI 768 GGCGCC GGC^GCC
1052 GGCGCC
SmaI 438 CCCGGG CCC^GGG
483 CCCGGG
SmlI 337 CTCAAG C^T[CT][GA]AG
913 CTCAAG
Sse8387I 882 CCTGCAGG CCTGCA^GG
978 CCTGCAGG
Tsp45I 10 GTGAC ^GT[GC]AC
287 GTGAC
XmaCI 438 CCCGGG C^CCGGG
483 CCCGGG
XmaI 438 CCCGGG C^CCGGG
483 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI AvaII BanI BanII BbvI
BcnI BmyI BsaHI BsaJI BsiEI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssHII BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EaeI EagI EarI EclXI
Eco52I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI Ksp632I
MboI MboII MnlI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PstI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TseI TspRI TthHB8I