Restriction map for
pF1KB9723
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaLI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbsI BbvCI BcgI BclI BfaI
BfrI BglII BlnI BpuAI BsaBI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I ClaI CpoI
DpnII DraI DrdI Eam1105I EarI
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI Ksp632I
MaeI MamI MboI MfeI MflI
MluI MseI MunI NdeI NdeII
NheI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SalI SapI Sau3AI SbfI
ScaI SexAI SfiI SfuI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tth111I
XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccIII 1008 TCCGGA T^CCGGA
AcsI 1084 GAATTT [GA]^AATT[CT]
ApoI 1084 GAATTT [GA]^AATT[CT]
AscI 628 GGCGCGCC GG^CGCGCC
Asp700I 216 GAACGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 521 TGGCCA TGG^CCA
BbrPI 880 CACGTG CAC^GTG
BpmI 541 CTCCAG CTGGAG(16/14)
BsaAI 880 CACGTG [CT]AC^GT[GA]
BsaI 765 GGTCTC GGTCTC(1/5)
BseAI 1008 TCCGGA T^CCGGA
BsmAI 766 GTCTC GTCTC(1/5)
BspEI 1008 TCCGGA T^CCGGA
BstAPI 378 GCAGCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 12 GGATG GGATG(2/0)
DraIII 1102 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1062 CCTCGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 12 GGATG GGATG(9/13)
MaeIII 29 GTGAC ^GT[ACGT]AC
MboII 707 TCTTC GAAGA(8/7)
MluNI 521 TGGCCA TGG^CCA
MroI 1008 TCCGGA T^CCGGA
MscI 521 TGGCCA TGG^CCA
MslI 123 CATGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 524 CCATGG C^CATGG
NspI 383 ACATGC [GA]CATG^[CT]
PflMI 618 CCAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 880 CACGTG CAC^GTG
PmlI 880 CACGTG CAC^GTG
PpuMI 837 GGGTCCC [GA]G^G[AT]CC[CT]
SacI 1057 GAGCTC GAGCT^C
SfcI 750 CTACAG C^T[GA][CT]AG
SgrAI 143 CGCCGGCG C[GA]^CCGG[CT]G
Tsp45I 29 GTGAC ^GT[GC]AC
Tsp509I 1085 AATT ^AATT
Van91I 618 CCAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 216 GAACGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 408 GTAC GT^AC
885 GTAC
AflIII 668 ACACGT A^C[GA][CT]GT
881 ACGTGT
AlwNI 619 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
1093 CAGTATCTG
AspHI 158 GAGCAC G[AT]GC[AT]^C
1057 GAGCTC
BbeI 141 GGCGCC GGCGC^C
861 GGCGCC
BciVI 19 GGATAC GTATCC(6/5)
200 GTATCC
BlpI 105 GCTGAGC GC^T[ACGT]AGC
478 GCTGAGC
Bpu1102I 105 GCTGAGC GC^T[ACGT]AGC
478 GCTGAGC
BsaWI 1008 TCCGGA [AT]^CCGG[AT]
1150 TCCGGT
BseRI 1060 CTCCTC GAGGAG(10/8)
355 GAGGAG
BsiHKAI 158 GAGCAC G[AT]GC[AT]^C
1057 GAGCTC
BsrBI 637 CCGCTC CCGCTC(-3/-3)
364 GAGCGG
BssHII 53 GCGCGC G^CGCGC
629 GCGCGC
CelII 105 GCTGAGC GC^T[ACGT]AGC
478 GCTGAGC
EcoT14I 185 CCAAGG C^C[AT][AT]GG
524 CCATGG
HgaI 327 GACGC GACGC(5/10)
1201 GACGC
KasI 141 GGCGCC G^GCGCC
861 GGCGCC
NarI 141 GGCGCC GG^CGCC
861 GGCGCC
NotI 490 GCGGCCGC GC^GGCCGC
512 GCGGCCGC
PshAI 992 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1039 GACCCCAGTC
RsaI 408 GTAC GT^AC
885 GTAC
SfoI 141 GGCGCC GGC^GCC
861 GGCGCC
StyI 185 CCAAGG C^C[AT][AT]GG
524 CCATGG
TaqI 641 TCGA T^CGA
844 TCGA
TspRI 27 CAGTG CA[GC]TG[ACGT][ACGT]^
1016 CACTG
TthHB8I 641 TCGA T^CGA
844 TCGA
XcmI 524 CCATGGACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1181 CCATTTCCTCGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AvaI AvaII BanI BanII BbvI
BcnI BglI BmyI BsaHI BsaJI
BsgI BsiEI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII DsaI
EaeI EagI EclXI Eco52I EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KspI MaeII
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NgoMIV NlaIII
NlaIV PleI SacII Sau96I ScrFI
SfaNI SmaI TfiI TseI XmaCI
XmaI