Restriction map for
pF1KB9726
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbrPI BbsI BciVI BclI
BfrI BglI BlnI BpuAI BsaI
BseAI BsgI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII DrdI
Eam1105I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI MaeIII MfeI
MluI MroI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp45I Tth111I Van91I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AlwNI 163 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1252 CCTCAGG CC^T[ACGT]AGG
Aor51HI 143 AGCGCT AGC^GCT
Asp700I 612 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 202 GAGCTC G[AT]GC[AT]^C
BalI 662 TGGCCA TGG^CCA
BamHI 1077 GGATCC G^GATCC
BbvCI 678 CCTCAGC CCTCAGC(-5/-2)
BcgI 71 GCAATGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 299 CTAG C^TAG
BglII 698 AGATCT A^GATCT
BlpI 146 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 146 GCTCAGC GC^T[ACGT]AGC
BsaAI 1054 TACGTG [CT]AC^GT[GA]
BsaBI 693 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1047 TCCGGT [AT]^CCGG[AT]
BsiEI 815 CGGCCG CG[GA][CT]^CG
BsiHKAI 202 GAGCTC G[AT]GC[AT]^C
BsmI 897 GAATGC GAATGC(1/-1)
BspHI 824 TCATGA T^CATGA
BspMI 668 ACCTGC ACCTGC(4/8)
BsrBI 685 GAGCGG CCGCTC(-3/-3)
BsrDI 71 GCAATG GCAATG(2/0)
BssHII 590 GCGCGC G^CGCGC
BssSI 585 CACGAG CACGAG(-5/-1)
BstAPI 798 GCAGCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 395 GGATG GGATG(2/0)
Bsu36I 1252 CCTCAGG CC^T[ACGT]AGG
CelII 146 GCTCAGC GC^T[ACGT]AGC
DraII 770 GGGGCCC [GA]G^G[ACGT]CC[CT]
EagI 815 CGGCCG C^GGCCG
EarI 40 CTCTTC CTCTTC(1/4)
EclXI 815 CGGCCG C^GGCCG
Eco47III 143 AGCGCT AGC^GCT
Eco52I 815 CGGCCG C^GGCCG
Eco81I 1252 CCTCAGG CC^T[ACGT]AGG
EcoNI 931 CCTCTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 770 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 746 CCAAGG C^C[AT][AT]GG
FokI 395 GGATG GGATG(9/13)
Ksp632I 40 CTCTTC CTCTTC(1/4)
KspI 29 CCGCGG CCGC^GG
MaeI 299 CTAG C^TAG
MamI 693 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 41 TCTTC GAAGA(8/7)
MluNI 662 TGGCCA TGG^CCA
MscI 662 TGGCCA TGG^CCA
MslI 892 CACCAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 186 ACATGC [GA]CATG^[CT]
PleI 403 GAGTC GAGTC(4/5)
PstI 160 CTGCAG CTGCA^G
RcaI 824 TCATGA T^CATGA
SacI 202 GAGCTC GAGCT^C
SacII 29 CCGCGG CCGC^GG
SfcI 160 CTGCAG C^T[GA][CT]AG
SmaI 546 CCCGGG CCC^GGG
StyI 746 CCAAGG C^C[AT][AT]GG
TfiI 1262 GAATC G^A[AT]TC
Tsp509I 1280 AATT ^AATT
XmaCI 546 CCCGGG C^CCGGG
XmaI 546 CCCGGG C^CCGGG
XmnI 612 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AlwI 1078 GATCC GGATC(4/5)
1077 GGATC
ApaI 322 GGGCCC GGGCC^C
771 GGGCCC
BsrI 275 CCAGT ACTGG(1/-1)
290 CCAGT
BstXI 119 CCACGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
746 CCAAGGGATTGG
BstYI 698 AGATCT [GA]^GATC[CT]
1077 GGATCC
EaeI 662 TGGCCA [CT]^GGCC[GA]
815 CGGCCG
HgaI 1026 GACGC GACGC(5/10)
559 GCGTC
HinfI 403 GAGTC G^A[ACGT]TC
1262 GAATC
MflI 698 AGATCT [GA]^GATC[CT]
1077 GGATCC
MseI 626 TTAA T^TAA
1282 TTAA
PvuII 213 CAGCTG CAG^CTG
595 CAGCTG
TaqI 86 TCGA T^CGA
406 TCGA
Tru9I 626 TTAA T^TAA
1282 TTAA
TthHB8I 86 TCGA T^CGA
406 TCGA
XhoII 698 AGATCT [GA]^GATC[CT]
1077 GGATCC
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BmyI BpmI BsaHI
BsaJI BseRI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrFI BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII HphI ItaI
KasI MaeII MboI MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfoI TseI TspRI