Restriction map for
pF1KB9731
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII BamHI
BbeI BbrPI BbsI BciVI BclI
BfrI BglI BglII BpmI BpuAI
BsaAI BsaBI BsaHI BsiWI BsmI
Bsp1407I BspHI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MseI MunI NarI
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
StuI SwaI TfiI Tru9I Tth111I
Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 22 GTCGAC GT^[AC][GT]AC
AccIII 251 TCCGGA T^CCGGA
AcsI 79 GAATTT [GA]^AATT[CT]
ApoI 79 GAATTT [GA]^AATT[CT]
Asp700I 354 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 222 CTCGAG C^[CT]CG[GA]G
AvrII 732 CCTAGG C^CTAGG
BalI 1088 TGGCCA TGG^CCA
BbvCI 1233 CCTCAGC CCTCAGC(-5/-2)
BcgI 39 CGACTACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 733 CTAG C^TAG
BlnI 732 CCTAGG C^CTAGG
BlpI 903 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 903 GCTGAGC GC^T[ACGT]AGC
BsaI 1291 GAGACC GGTCTC(1/5)
BseAI 251 TCCGGA T^CCGGA
BseRI 255 GAGGAG GAGGAG(10/8)
BsiEI 972 CGACCG CG[GA][CT]^CG
BsmBI 504 CGTCTC CGTCTC(1/5)
BsoBI 222 CTCGAG C^[CT]CG[GA]G
BspDI 841 ATCGAT AT^CGAT
BspEI 251 TCCGGA T^CCGGA
BspLU11I 275 ACATGT A^CATGT
BsrBI 397 GAGCGG CCGCTC(-3/-3)
BsrFI 296 GCCGGC [GA]^CCGG[CT]
BstXI 152 CCAACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 903 GCTGAGC GC^T[ACGT]AGC
Cfr10I 296 GCCGGC [GA]^CCGG[CT]
ClaI 841 ATCGAT AT^CGAT
DrdI 742 GACTACATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1078 CTCTTC CTCTTC(1/4)
HaeII 169 AGCGCC [GA]GCGC^[CT]
Ksp632I 1078 CTCTTC CTCTTC(1/4)
MaeI 733 CTAG C^TAG
MaeIII 230 GTGAC ^GT[ACGT]AC
MluNI 1088 TGGCCA TGG^CCA
MroI 251 TCCGGA T^CCGGA
MscI 1088 TGGCCA TGG^CCA
MspA1I 580 CAGCTG C[AC]G^C[GT]G
NaeI 296 GCCGGC GCC^GGC
NcoI 707 CCATGG C^CATGG
NdeI 1189 CATATG CA^TATG
NgoMIV 296 GCCGGC G^CCGGC
NspI 275 ACATGT [GA]CATG^[CT]
PaeR7I 222 CTCGAG C^TCGAG
PvuII 580 CAGCTG CAG^CTG
SacI 628 GAGCTC GAGCT^C
SalI 22 GTCGAC G^TCGAC
SapI 1077 GCTCTTC GCTCTTC(1/4)
SbfI 465 CCTGCAGG CCTGCA^GG
SexAI 644 ACCAGGT A^CC[AT]GGT
Sse8387I 465 CCTGCAGG CCTGCA^GG
Tsp45I 230 GTGAC ^GT[GC]AC
Tsp509I 80 AATT ^AATT
XcmI 698 CCAAGAGATCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 222 CTCGAG C^TCGAG
XmnI 354 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 147 CGCG CG^CG
989 CGCG
AfaI 279 GTAC GT^AC
609 GTAC
AflIII 116 ACACGT A^C[GA][CT]GT
275 ACATGT
AspHI 628 GAGCTC G[AT]GC[AT]^C
676 GAGCAC
BanI 133 GGCACC G^G[CT][GA]CC
217 GGTGCC
BsaWI 251 TCCGGA [AT]^CCGG[AT]
404 ACCGGA
BsiHKAI 628 GAGCTC G[AT]GC[AT]^C
676 GAGCAC
BsmAI 1291 GAGAC GTCTC(1/5)
505 GTCTC
BsrDI 110 GCAATG GCAATG(2/0)
737 GCAATG
BstUI 147 CGCG CG^CG
989 CGCG
BstYI 703 AGATCC [GA]^GATC[CT]
1061 AGATCC
DraII 476 AGGTCCT [GA]G^G[ACGT]CC[CT]
1129 GGGTCCC
DsaI 200 CCACGG C^C[GA][CT]GG
707 CCATGG
EcoNI 1104 CCTGTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1176 CCTGATGCAGG
EcoO109I 476 AGGTCCT [GA]G^G[ACGT]CC[CT]
1129 GGGTCCC
FauI 188 GCGGG CCCGC(4/6)
399 GCGGG
HincII 22 GTCGAC GT[CT]^[GA]AC
99 GTTGAC
HindII 22 GTCGAC GT[CT]^[GA]AC
99 GTTGAC
MflI 703 AGATCC [GA]^GATC[CT]
1061 AGATCC
MslI 27 CATGGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
211 CACTACGGTG
MvnI 147 CGCG CG^CG
989 CGCG
PpuMI 476 AGGTCCT [GA]G^G[AT]CC[CT]
1129 GGGTCCC
RsaI 279 GTAC GT^AC
609 GTAC
SmlI 222 CTCGAG C^T[CT][GA]AG
349 CTCAAG
XhoII 703 AGATCC [GA]^GATC[CT]
1061 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanII BbvI BcnI BmyI BsaJI
BsgI BsiYI BslI BsmFI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MboI MboII MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
TspRI TthHB8I