Restriction map for
pF1KB9734
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AocI ApoI AscI
AseI AsnI Asp700I Asp718I AvrII
BamHI BbsI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsaWI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KpnI MamI MfeI MluI MroI
MseI MunI NcoI NdeI NheI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmeI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SalI SapI SbfI ScaI
SexAI SfiI SfuI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI TfiI Tru9I TthHB8I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 541 GACGTC GACGT^C
AfaI 917 GTAC GT^AC
AflIII 1322 ACGTGT A^C[GA][CT]GT
AhdI 724 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1375 GTGCAC G^TGCAC
AlwNI 508 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 641 GGGCCC GGGCC^C
ApaLI 1375 GTGCAC G^TGCAC
AspEI 724 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 586 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 816 CCCGGG C^[CT]CG[GA]G
BalI 911 TGGCCA TGG^CCA
BbrPI 1282 CACGTG CAC^GTG
BfaI 869 CTAG C^TAG
BsaAI 1282 CACGTG [CT]AC^GT[GA]
BsgI 1016 CTGCAC GTGCAG(16/14)
BsoBI 816 CCCGGG C^[CT]CG[GA]G
BspMI 1199 ACCTGC ACCTGC(4/8)
BsrBI 1405 GAGCGG CCGCTC(-3/-3)
BstAPI 1100 GCACGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eam1105I 724 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 301 CTCTTC CTCTTC(1/4)
EcoNI 130 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Ksp632I 301 CTCTTC CTCTTC(1/4)
MaeI 869 CTAG C^TAG
MluNI 911 TGGCCA TGG^CCA
MscI 911 TGGCCA TGG^CCA
MslI 1278 CACGCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 756 GCCGGC GCC^GGC
NgoMIV 756 GCCGGC G^CCGGC
NlaIII 312 CATG CATG^
PflFI 586 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1282 CACGTG CAC^GTG
PmlI 1282 CACGTG CAC^GTG
PpuMI 606 GGGTCCC [GA]G^G[AT]CC[CT]
RsaI 917 GTAC GT^AC
SacI 1186 GAGCTC GAGCT^C
SfcI 243 CTACAG C^T[GA][CT]AG
SgrAI 1296 CACCGGCG C[GA]^CCGG[CT]G
SmaI 816 CCCGGG CCC^GGG
Tsp509I 83 AATT ^AATT
Tth111I 586 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 816 CCCGGG C^CCGGG
XmaI 816 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AlwI 368 GATCC GGATC(4/5)
441 GGATC
Aor51HI 76 AGCGCT AGC^GCT
579 AGCGCT
AspHI 1186 GAGCTC G[AT]GC[AT]^C
1375 GTGCAC
AviII 1202 TGCGCA TGC^GCA
1286 TGCGCA
BbeI 561 GGCGCC GGCGC^C
1079 GGCGCC
BpmI 131 CTGGAG CTGGAG(16/14)
205 CTGGAG
BseRI 37 CTCCTC GAGGAG(10/8)
1402 GAGGAG
BsiHKAI 1186 GAGCTC G[AT]GC[AT]^C
1375 GTGCAC
BsmAI 668 GAGAC GTCTC(1/5)
592 GTCTC
BsrI 914 CCAGT ACTGG(1/-1)
1230 CCAGT
BstYI 367 AGATCC [GA]^GATC[CT]
441 GGATCT
DpnII 368 GATC ^GATC
442 GATC
Eco47III 76 AGCGCT AGC^GCT
579 AGCGCT
EcoT14I 1061 CCAAGG C^C[AT][AT]GG
1130 CCAAGG
FspI 1202 TGCGCA TGC^GCA
1286 TGCGCA
HinfI 193 GACTC G^A[ACGT]TC
776 GACTC
HphI 820 GGTGA GGTGA(8/7)
1274 TCACC
KasI 561 GGCGCC G^GCGCC
1079 GGCGCC
MaeIII 660 GTCAC ^GT[ACGT]AC
821 GTGAC
MboI 368 GATC ^GATC
442 GATC
MflI 367 AGATCC [GA]^GATC[CT]
441 GGATCT
NarI 561 GGCGCC GG^CGCC
1079 GGCGCC
NdeII 368 GATC ^GATC
442 GATC
NotI 1119 GCGGCCGC GC^GGCCGC
1328 GCGGCCGC
PleI 193 GACTC GAGTC(4/5)
776 GACTC
PvuII 165 CAGCTG CAG^CTG
738 CAGCTG
Sau3AI 368 GATC ^GATC
442 GATC
SfoI 561 GGCGCC GGC^GCC
1079 GGCGCC
StyI 1061 CCAAGG C^C[AT][AT]GG
1130 CCAAGG
Tsp45I 660 GTCAC ^GT[GC]AC
821 GTGAC
XcmI 365 CCAGATCCCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
463 CCAGAGGCGTTCTGG
XhoII 367 AGATCC [GA]^GATC[CT]
441 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AcyI AluI AvaII
BanI BanII BbvCI BbvI BcnI
BglI BmyI BsaHI BsaJI BsiEI
BsiYI BslI BsmFI Bsp1286I BsrFI
BssHII BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII DsaI EaeI EagI EclXI
Eco52I EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII ItaI
KspI MaeII MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIV SacII Sau96I ScrFI SfaNI
SmlI StuI TseI TspRI