Restriction map for
pF1KB9737
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbsI
BclI BfrI BglII BlnI BpuAI
BsaAI BsaI BsaWI BseAI BsiWI
BsmBI BsmI BspEI BspLU11I BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Cfr10I CpoI DraI DraIII
DsaI Eam1105I Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MaeII MfeI MluI MroI
MseI MslI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tru9I
Tth111I XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccII 942 CGCG CG^CG
AcsI 599 AAATTT [GA]^AATT[CT]
AlwI 1074 GGATC GGATC(4/5)
AocI 697 CCTGAGG CC^T[ACGT]AGG
ApoI 599 AAATTT [GA]^AATT[CT]
AvaI 72 CTCGGG C^[CT]CG[GA]G
BalI 1486 TGGCCA TGG^CCA
BbeI 190 GGCGCC GGCGC^C
BcgI 228 CGAGGACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1447 GGATAC GTATCC(6/5)
BfaI 1078 CTAG C^TAG
BsaBI 840 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1205 CGGCCG CG[GA][CT]^CG
BsmAI 637 GTCTC GTCTC(1/5)
BsoBI 72 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1064 TGTACA T^GTACA
BspDI 79 ATCGAT AT^CGAT
BspHI 479 TCATGA T^CATGA
BsrBI 870 CCGCTC CCGCTC(-3/-3)
BsrDI 324 CATTGC GCAATG(2/0)
BsrGI 1064 TGTACA T^GTACA
BstUI 942 CGCG CG^CG
BstXI 158 CCAACGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1074 GGATCT [GA]^GATC[CT]
Bsu36I 697 CCTGAGG CC^T[ACGT]AGG
ClaI 79 ATCGAT AT^CGAT
DrdI 51 GACTGAGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1205 CGGCCG C^GGCCG
EarI 1270 CTCTTC CTCTTC(1/4)
EclXI 1205 CGGCCG C^GGCCG
Eco52I 1205 CGGCCG C^GGCCG
Eco81I 697 CCTGAGG CC^T[ACGT]AGG
EcoNI 1298 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HphI 440 TCACC GGTGA(8/7)
KasI 190 GGCGCC G^GCGCC
Ksp632I 1270 CTCTTC CTCTTC(1/4)
MaeI 1078 CTAG C^TAG
MamI 840 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1074 GGATCT [GA]^GATC[CT]
MluNI 1486 TGGCCA TGG^CCA
MscI 1486 TGGCCA TGG^CCA
MvnI 942 CGCG CG^CG
NarI 190 GGCGCC GG^CGCC
PflMI 1292 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1100 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 479 TCATGA T^CATGA
SacI 955 GAGCTC GAGCT^C
SfiI 1206 GGCCGCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 190 GGCGCC GGC^GCC
SspBI 1064 TGTACA T^GTACA
TfiI 1057 GATTC G^A[AT]TC
Tsp509I 600 AATT ^AATT
Van91I 1292 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1077 TCTAGA T^CTAGA
XhoII 1074 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 118 GACGCC G[GA]^CG[CT]C
190 GGCGCC
AlwNI 1090 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1099 CAGGACCTG
AspHI 928 GAGCAC G[AT]GC[AT]^C
955 GAGCTC
AvaII 810 GGACC G^G[AT]CC
1101 GGACC
BbvCI 87 CCTCAGC CCTCAGC(-5/-2)
904 GCTGAGG
BglI 101 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1207 GCCGCCAAGGC
BlpI 655 GCTGAGC GC^T[ACGT]AGC
1134 GCTGAGC
Bpu1102I 655 GCTGAGC GC^T[ACGT]AGC
1134 GCTGAGC
BsaHI 118 GACGCC G[GA]^CG[CT]C
190 GGCGCC
BsgI 1108 CTGCAC GTGCAG(16/14)
1219 GTGCAG
BsiHKAI 928 GAGCAC G[AT]GC[AT]^C
955 GAGCTC
BssSI 1111 CACGAG CACGAG(-5/-1)
310 CTCGTG
CelII 655 GCTGAGC GC^T[ACGT]AGC
1134 GCTGAGC
Hin1I 118 GACGCC G[GA]^CG[CT]C
190 GGCGCC
MaeIII 731 GTGAC ^GT[ACGT]AC
1024 GTGAC
MboII 377 TCTTC GAAGA(8/7)
1271 TCTTC
NspI 278 ACATGC [GA]CATG^[CT]
1380 GCATGT
Tsp45I 731 GTGAC ^GT[GC]AC
1024 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI BanI BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII ItaI MboI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TseI TspRI TthHB8I XcmI